DNAC_ECOLI
ID DNAC_ECOLI Reviewed; 245 AA.
AC P0AEF0; P07905; Q2M5V5;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=DNA replication protein DnaC;
GN Name=dnaC; Synonyms=dnaD; OrderedLocusNames=b4361, JW4325;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=3301836; DOI=10.1016/s0021-9258(18)60985-8;
RA Nakayama N., Bond M.W., Miyajima A., Kobori J., Arai K.;
RT "Structure of Escherichia coli dnaC. Identification of a cysteine residue
RT possibly involved in association with dnaB protein.";
RL J. Biol. Chem. 262:10475-10480(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2844800; DOI=10.1016/s0021-9258(18)68149-9;
RA Masai H., Arai K.;
RT "Operon structure of dnaT and dnaC genes essential for normal and stable
RT DNA replication of Escherichia coli chromosome.";
RL J. Biol. Chem. 263:15083-15093(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-14.
RC STRAIN=K12;
RX PubMed=3006041; DOI=10.1073/pnas.83.5.1256;
RA Masai H., Bond M.W., Arai K.;
RT "Cloning of the Escherichia coli gene for primosomal protein I: the
RT relationship to dnaT, essential for chromosomal DNA replication.";
RL Proc. Natl. Acad. Sci. U.S.A. 83:1256-1260(1986).
RN [7]
RP IDENTIFICATION BY 2D-GEL.
RX PubMed=9298644; DOI=10.1002/elps.1150180805;
RA VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT "Escherichia coli proteome analysis using the gene-protein database.";
RL Electrophoresis 18:1243-1251(1997).
RN [8]
RP INDUCTION BY HYDROXYUREA.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=20005847; DOI=10.1016/j.molcel.2009.11.024;
RA Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J.,
RA Walker G.C.;
RT "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia
RT coli.";
RL Mol. Cell 36:845-860(2009).
CC -!- FUNCTION: This protein is required for chromosomal replication. It
CC forms, in concert with DnaB protein and other prepriming proteins DnaT,
CC N, N', N'' a prepriming protein complex on the specific site of the
CC template DNA recognized by protein N'.
CC -!- INTERACTION:
CC P0AEF0; P0ACB0: dnaB; NbExp=12; IntAct=EBI-549012, EBI-548978;
CC -!- INDUCTION: Induced by hydroxyurea. {ECO:0000269|PubMed:20005847}.
CC -!- SIMILARITY: Belongs to the DnaC family. {ECO:0000305}.
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DR EMBL; J04030; AAA23700.1; -; Genomic_DNA.
DR EMBL; U14003; AAA97260.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77317.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78351.1; -; Genomic_DNA.
DR PIR; A28484; XMECNC.
DR RefSeq; NP_418781.1; NC_000913.3.
DR RefSeq; WP_000799911.1; NZ_STEB01000025.1.
DR PDB; 6KZA; X-ray; 3.10 A; C/D=1-56.
DR PDB; 6QEL; EM; 3.90 A; G/H/I/J/K/L=1-245.
DR PDB; 6QEM; EM; 3.40 A; G/H/I/J/K/L=1-245.
DR PDBsum; 6KZA; -.
DR PDBsum; 6QEL; -.
DR PDBsum; 6QEM; -.
DR AlphaFoldDB; P0AEF0; -.
DR SMR; P0AEF0; -.
DR BioGRID; 4262755; 8.
DR BioGRID; 853143; 1.
DR ComplexPortal; CPX-1934; dnaB-dnaC complex.
DR ComplexPortal; CPX-1950; dnaA-dnaB-dnaC loader complex.
DR ComplexPortal; CPX-1951; Replication restart pre-primosome complex, priAB variant.
DR ComplexPortal; CPX-1952; Replication restart pre-primosome complex, priAC variant.
DR ComplexPortal; CPX-1953; Replication restart pre-primosome complex priC-rep variant.
DR ComplexPortal; CPX-5909; Replication restart primosome complex, priAC variant.
DR ComplexPortal; CPX-5910; Replication restart primosome complex, priAB variant.
DR ComplexPortal; CPX-5911; Replication restart primosome complex, priC-rep variant.
DR DIP; DIP-9457N; -.
DR IntAct; P0AEF0; 13.
DR STRING; 511145.b4361; -.
DR jPOST; P0AEF0; -.
DR PaxDb; P0AEF0; -.
DR PRIDE; P0AEF0; -.
DR EnsemblBacteria; AAC77317; AAC77317; b4361.
DR EnsemblBacteria; BAE78351; BAE78351; BAE78351.
DR GeneID; 66671753; -.
DR GeneID; 948864; -.
DR KEGG; ecj:JW4325; -.
DR KEGG; eco:b4361; -.
DR PATRIC; fig|1411691.4.peg.2325; -.
DR EchoBASE; EB0233; -.
DR eggNOG; COG1484; Bacteria.
DR HOGENOM; CLU_062999_3_1_6; -.
DR InParanoid; P0AEF0; -.
DR OMA; VGDLMKR; -.
DR PhylomeDB; P0AEF0; -.
DR BioCyc; EcoCyc:EG10237-MON; -.
DR PRO; PR:P0AEF0; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:1990100; C:DnaB-DnaC complex; IPI:ComplexPortal.
DR GO; GO:1990158; C:DnaB-DnaC-DnaT-PriA-PriB complex; IC:ComplexPortal.
DR GO; GO:1990159; C:DnaB-DnaC-DnaT-PriA-PriC complex; IC:ComplexPortal.
DR GO; GO:1990160; C:DnaB-DnaC-Rep-PriC complex; IC:ComplexPortal.
DR GO; GO:1990077; C:primosome complex; IC:ComplexPortal.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR GO; GO:0006270; P:DNA replication initiation; IDA:ComplexPortal.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IC:ComplexPortal.
DR GO; GO:0006271; P:DNA strand elongation involved in DNA replication; IBA:GO_Central.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IC:ComplexPortal.
DR GO; GO:0031297; P:replication fork processing; IC:ComplexPortal.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR028350; DNAC/ATP-binding.
DR InterPro; IPR002611; IstB_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01695; IstB_IS21; 1.
DR PIRSF; PIRSF003073; DNAC_TnpB_IstB; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; DNA replication; Nucleotide-binding; Primosome;
KW Reference proteome.
FT CHAIN 1..245
FT /note="DNA replication protein DnaC"
FT /id="PRO_0000079954"
FT SITE 69
FT /note="Probably involved in the interaction with the DnaB
FT protein"
FT HELIX 5..13
FT /evidence="ECO:0007829|PDB:6KZA"
FT HELIX 26..49
FT /evidence="ECO:0007829|PDB:6KZA"
FT STRAND 57..59
FT /evidence="ECO:0007829|PDB:6QEM"
FT HELIX 64..66
FT /evidence="ECO:0007829|PDB:6QEM"
FT HELIX 79..93
FT /evidence="ECO:0007829|PDB:6QEM"
FT STRAND 102..106
FT /evidence="ECO:0007829|PDB:6QEM"
FT STRAND 108..111
FT /evidence="ECO:0007829|PDB:6QEM"
FT HELIX 112..126
FT /evidence="ECO:0007829|PDB:6QEM"
FT STRAND 130..134
FT /evidence="ECO:0007829|PDB:6QEM"
FT HELIX 135..147
FT /evidence="ECO:0007829|PDB:6QEM"
FT HELIX 153..161
FT /evidence="ECO:0007829|PDB:6QEM"
FT STRAND 163..170
FT /evidence="ECO:0007829|PDB:6QEM"
FT HELIX 178..192
FT /evidence="ECO:0007829|PDB:6QEM"
FT TURN 193..195
FT /evidence="ECO:0007829|PDB:6QEM"
FT STRAND 199..201
FT /evidence="ECO:0007829|PDB:6QEM"
FT HELIX 206..210
FT /evidence="ECO:0007829|PDB:6QEM"
FT HELIX 215..221
FT /evidence="ECO:0007829|PDB:6QEM"
FT STRAND 228..230
FT /evidence="ECO:0007829|PDB:6QEM"
FT HELIX 236..238
FT /evidence="ECO:0007829|PDB:6QEM"
SQ SEQUENCE 245 AA; 27935 MW; C5CC7232E21F11F1 CRC64;
MKNVGDLMQR LQKMMPAHIK PAFKTGEELL AWQKEQGAIR SAALERENRA MKMQRTFNRS
GIRPLHQNCS FENYRVECEG QMNALSKARQ YVEEFDGNIA SFIFSGKPGT GKNHLAAAIC
NELLLRGKSV LIITVADIMS AMKDTFRNSG TSEEQLLNDL SNVDLLVIDE IGVQTESKYE
KVIINQIVDR RSSSKRPTGM LTNSNMEEMT KLLGERVMDR MRLGNSLWVI FNWDSYRSRV
TGKEY
