ID   DNAE2_ALKEH             Reviewed;        1025 AA.
AC   Q0A9G3;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=Mlg_1175;
OS   Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales;
OC   Ectothiorhodospiraceae; Alkalilimnicola.
OX   NCBI_TaxID=187272;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1101 / DSM 17681 / MLHE-1;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S.,
RA   Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B.,
RA   Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P.;
RT   "Complete sequence of Alkalilimnicola ehrilichei MLHE-1.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; CP000453; ABI56524.1; -; Genomic_DNA.
DR   RefSeq; WP_011628919.1; NC_008340.1.
DR   AlphaFoldDB; Q0A9G3; -.
DR   SMR; Q0A9G3; -.
DR   EnsemblBacteria; ABI56524; ABI56524; Mlg_1175.
DR   KEGG; aeh:Mlg_1175; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_6; -.
DR   OMA; NSWPMGF; -.
DR   OrthoDB; 561611at2; -.
DR   Proteomes; UP000001962; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1025
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_1000070587"
SQ   SEQUENCE   1025 AA;  114101 MW;  D87F812D48EFE07A CRC64;
     MPIDYAELHC LSCFSFLRGA SQPAELVQRA AELGYRALAL TDACSVAGAV RAHQAAKETD
     LHLIHGSEIR IHQGPLLVLL APCRRAWAEL CALISLGRSQ ARKGDYRLER EQLEGTLPHC
     LALWVPDDAP HDAEQGRWFA RHFGDRGHVA VALHHGPDDE ARLQRLLALA DRFRLPAVAA
     GGVLMHRRGR RALQDTLSAL RHRRTLAAMG TALECSGERH LRSLHSLARL YPPALLRRSV
     HLADQCRFSL DKLRYEYPAE LVPAGETPAS YLRRLTLEGA RRHWPQGMPD KVAHQVDHEL
     ALIAEMGYEP FFLTVHDVVA FARRRGILCQ GRGSSANSAV CFCLGITAVD PARQSLLFER
     FISKERGEPP DIDVDFEHER REEVIQYIYR KYGRHRAALA ATVIRYRPRS ALRDAGRALG
     LDAATIDRLA GSIQWWDGKR VDPERLREAG LNPDDPRLAR TVAIAGQLLG LPRHLSQHVG
     GFVISEGPIS ELVPTENAAM AGRTIIQWDK DDLEALGLLK VDVLALGMLS CIRRAFDLLA
     GFRGRRLTLA DVPAEDPAVY RMISDADTMG VFQIESRAQM AMLPRLRPQT FYDLVIEVAI
     VRPGPIQGDM VHPYLRRREG LEPVDYPSEA VRGVLARTLG VPIFQEQVMQ LAVVAAGFTP
     GEADALRRAM AAWKRKGGLG PFRDKLLKGM RRNGYCEDYA ERLFRQIQGF GEYGFPESHA
     ASFALLVYVS AWLKCHEPAL FTCALLNSQP MGFYAPAQLL RDAERHGVEI RPVDVRHSDW
     DCSPEHRGDG EPALRLGLRL VRGLNRRAAD RLIAARGRRP FRDVQEMARR AALHRRDLET
     LAHAGALRGL AGHRRAAWWQ VLGAEAGLPV FEDLHIEEAA PALDAPAEGE DLVADYTSLG
     FTLGRHPLAL LRPQLRRRRL LTAADLASTG HGRLVRTAGL VINRQRPGSA GGVTFLTLED
     ETGQINLVVW KATAEAQRRT LLAARLLMVS GIWERKGAVT HLVAGRLEDW SDWLGALDVR
     SRDFH