ID   DNAE2_ANAD2             Reviewed;        1142 AA.
AC   B8JAF5;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=A2cp1_2336;
OS   Anaeromyxobacter dehalogenans (strain 2CP-1 / ATCC BAA-258).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Anaeromyxobacteraceae; Anaeromyxobacter.
OX   NCBI_TaxID=455488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2CP-1 / ATCC BAA-258;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C.,
RA   Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA   Beliaev A.S., Richardson P.;
RT   "Complete sequence of Anaeromyxobacter dehalogenans 2CP-1.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; CP001359; ACL65674.1; -; Genomic_DNA.
DR   RefSeq; WP_012633501.1; NC_011891.1.
DR   AlphaFoldDB; B8JAF5; -.
DR   SMR; B8JAF5; -.
DR   EnsemblBacteria; ACL65674; ACL65674; A2cp1_2336.
DR   KEGG; acp:A2cp1_2336; -.
DR   HOGENOM; CLU_001600_4_0_7; -.
DR   OMA; NSWPMGF; -.
DR   Proteomes; UP000007089; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1600; -; 1.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 2.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 2.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1142
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_1000188730"
FT   REGION          291..361
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1142 AA;  121997 MW;  7D40F902D5217524 CRC64;
     MSHAPRYAEL RCKSCFSFLE GASHPEELVG RAAELGLSAL ALADVNGLYG IVRAHAEAKR
     QGLPLIVGAE LVVAGLAPGR PARLVLLAQD REGYAGLCRL VTRAHCGEGW TGAPERRERD
     AVAVPFEAVA AGARGLFALY PGADGDAVAR LKDAFGRRAA LAVTRHRVAG EEARVLAARS
     AGRRLGVPVA VTNDVHTHAR ARQVLQDVLT CVRHGTTVDR AGRRLFPNAE RTLKGPEELA
     RLWSDFPEGL AAAADIADQC RFRMEEIRGE HPLPPVVVER GALAGGVEVA TSSPAQAARE
     GARTATPSLS LRASLPAERP AAPEPEGPAA SAPEGPASSE PGEPGLAGAG GGTGAAAGTD
     RDGALAGMSL LRELVREGAR WRYGGEPPED VARQLARELD LVESLGYASY FLTVWDVVRF
     ARSRGILCQG RGSAANSAVC YVLGITSIDP VRMGLLFERF ISAERGEPPD IDVDFEHERR
     EEVLQYVYQR YGRDRAGMVC EVITYRGKSA LRDVGKALGL SLGQVDRLAK LIGTYEDLGQ
     VGPELLAQAG LDAADSERVR MTLALARELQ GFPRHLSIHV GGFVITRRPL CETVPIEPAA
     MPGRTIVQWD KDDLSELDLL KVDLLGLGML TALSRALALL ARHRPAPASP TAVPHPDALA
     TIPAEDPEVY EMLGRADSIG VFQVESRAQM SLAPRLRPRN FYDLVISVAI IRPGPIQGGM
     IHPYLRRRDG KEQVRYPYAP LEPVLARTLG VPLFQEQAMR LAVIAAGFTP GEADELRRVM
     THRRSHEKLA AMKARLVAGM AERGISGADA EEIFKQLLGF AGYGFPESHA ASFALLVYAS
     AWLKRYHPAA FACALLNSQP MGFYAPHTLV EDAKRHGVEV RGVDVGCSGW ESSLEGAAPG
     RPAAPGETAV LRVGLHAVRG LPRAVGEAIL EARAAGPFGS VAELVRRARL SRAWLVRLAE
     AGALGALAPD RRDAVWRSLA VEADGGDLFA GLAPPEPEVA LPAASAADEV SADFATTGLS
     VRGHPMALVR PGLGGDRIRT ARELGRLPDR APVEVAGLVI VRQRPETARG IVFVSLEDET
     GIANLVVMPD VYERFRPVVR GAPFLLARGR VERSGKVVNV RVDSVAPLAL APSMGARARD
     FH