ID   DNAE2_AROAE             Reviewed;        1070 AA.
AC   Q5P4A4;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   OrderedLocusNames=AZOSEA17340; ORFNames=ebA3077;
OS   Aromatoleum aromaticum (strain EbN1) (Azoarcus sp. (strain EbN1)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales;
OC   Rhodocyclaceae; Aromatoleum.
OX   NCBI_TaxID=76114;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EbN1;
RX   PubMed=15551059; DOI=10.1007/s00203-004-0742-9;
RA   Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F.,
RA   Reinhardt R.;
RT   "The genome sequence of an anaerobic aromatic-degrading denitrifying
RT   bacterium, strain EbN1.";
RL   Arch. Microbiol. 183:27-36(2005).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; CR555306; CAI07859.1; -; Genomic_DNA.
DR   RefSeq; WP_011237573.1; NC_006513.1.
DR   AlphaFoldDB; Q5P4A4; -.
DR   SMR; Q5P4A4; -.
DR   STRING; 76114.ebA3077; -.
DR   PRIDE; Q5P4A4; -.
DR   EnsemblBacteria; CAI07859; CAI07859; ebA3077.
DR   KEGG; eba:ebA3077; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_4; -.
DR   OMA; NSWPMGF; -.
DR   OrthoDB; 561611at2; -.
DR   Proteomes; UP000006552; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1070
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103365"
SQ   SEQUENCE   1070 AA;  117515 MW;  659B91336FE4881E CRC64;
     MDSAPPSPSP SPAPAYAELH CLTNFSFQRG ASHPEELVAR AAEAGYTALA ITDECSLAGV
     VRAHQQLRRL ARPLKLLIGS ELRLVDGLRV VLLATDRAAY GRLARLITIG RRAATKGRYQ
     LTCADLDRGI PGCLALVVPP DDRILDAAAL VADARWVGER FPDAAWLAVE LACGPDDGRR
     LEALLAIASA AGLPPVAATG ALMHDPSRRA LADVLAALRL RVTIAEAGRA LAPNAERALH
     ERAVLARRYP AELLAETLRI VARCHFRLDE LRYEYPAELV PDGETPASWL RRLVEDGLRW
     RHGRPQDDLA PPKVRAQVEH ELALIAELGF EAYFLTVEDL VRFARGRGIL CQGRGSAANS
     VICWALGITE VDPELGIMLV ERFISKERDE PPDIDVDFEH ERREEVIQYV YRKYGRERAA
     LAATVIRYRA RSALRDVGRA LGLPPGQLER LARDRFWFDA GRIRPERLRE AGFDPASPGV
     QRLATLTEAL VGFPRHLSQH VGGFVIARGR LDELVPVENA AMPERTVIQW DKDDLDALGL
     LKIDVLALGM LSALRRSLAL VSTWRGRPFT LADIPREAPE VYRMLSEADS IGVFQIESRA
     QMTMLPRLKP ARFYDLVVQV AIVRPGPIQG GMVHPYLQAR ARIERDEPVS YPQPHCPDAD
     ADGTCVPRPD DVRRVLERTL GVPIFQEQVM QLAVVAAGFT PGEADQLRRA MGAFRRHGEL
     ERYRAKLLAG MAARGYRAEF AERLCDQIEG FGSYGFPESH AASFALLVYC SAWLKCCEPA
     AFLCGLLNSQ PMGFYAPSQL IQDARRHGVV VLGAEVTASD WDCTLEALPD ASPGAAPAVR
     LGLQLVKGFG REAAMRIVAA RTERPFPNVD ELALRARLDA AALKRLAEAG ALEPLAGHRR
     QALWQAAAGH APEGVLRGAR IAEPRAELAA PSEAQELVAD YARLGFTLGR HPLALLRAKL
     TTLRFVTAAE IRDYPDRKLA RIAGLVTCRQ RPGTAKGTLF LTVEDETGLA NVIVQAELVE
     RQRREVLGAR LLGVFGQIRS KGQVVHLLAQ RLVDHSALLG ALEARSRDFQ