ID   DNAE2_AZOSB             Reviewed;        1064 AA.
AC   A1KCL7;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=azo3957;
OS   Azoarcus sp. (strain BH72).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC   Azoarcus.
OX   NCBI_TaxID=418699;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BH72;
RX   PubMed=17057704; DOI=10.1038/nbt1243;
RA   Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J.,
RA   Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C.,
RA   Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J.,
RA   Weidner S., Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.;
RT   "Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp.
RT   strain BH72.";
RL   Nat. Biotechnol. 24:1385-1391(2006).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; AM406670; CAL96573.1; -; Genomic_DNA.
DR   RefSeq; WP_011767679.1; NC_008702.1.
DR   AlphaFoldDB; A1KCL7; -.
DR   SMR; A1KCL7; -.
DR   STRING; 62928.azo3957; -.
DR   EnsemblBacteria; CAL96573; CAL96573; azo3957.
DR   KEGG; azo:azo3957; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_4; -.
DR   OMA; NSWPMGF; -.
DR   OrthoDB; 561611at2; -.
DR   Proteomes; UP000002588; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1064
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_1000070588"
SQ   SEQUENCE   1064 AA;  116192 MW;  1131DA75605D8C75 CRC64;
     MATPPYAELH CLSNYSFLRG ASHPEELVAR AWALGYQGLA ITDECSLAGA VRAHTGLAEL
     RRDLRAAADA PEASAQDRLL AERAEQFRLV FGAEFRLACG LKLVLLAKNR AGYGNLSALI
     TLARQRSEKG SYRLVRADLD AISPAGAVPD CYALWLPDAA SSAEDAHWFA RRFGERGWLA
     VELHSGADDA AWLGRTAELA AAAGLPRLAA GDVHMHLRGR RPLQDTLTAI RLGTTVFEAG
     TALHPNGERH LRHPLRLARL YPAELLEAAA RLALACDFQL DTLRYEYPQE IVPAGETPAS
     YLRRETQAGL ARRYPQGVPP AIADNVEQEL ALIIELGYEP FFLTVYDIVC FARDEGILCQ
     GRGSAANSIV CYALGITEVD PARASLLFGR FISRERDEPP DIDVDFEHDR RETVIQYIYG
     KYGRDRAALA ATVIRYRTRG ALRDAGRALG FGQPQIDALA RSLAWWDKRD QLPARLIELG
     LDPASPRVAK WLDLTGMLIG FPRHLSQHVG GFVISRGPLG RLVPIENAAM PERSVIQWDK
     EDLEALGLLK VDVLALGMLS VIRRSLELVG RRRGAPFALP HIPPKDAATF DMLCAADSVG
     VFQVESRAQM AMLPRLRPRQ FYDLVVQVAI VRPGPIQGDM VHPYLTNRAD PAASARTLAR
     LPADVRAVLE RTLGVPIFQE QVMKLAEVAA GFTPGEADQL RRAMASWRQK GHIERFKQKL
     RDGMKARGHD SDFADALCRQ IEGFGEYGFP ESHAASFALL AYASAWLKRH EPEAFLCGLL
     NSQPMGFYAP AQLLQDARRH GVEVRPVDVT ASAWDATLED LPADPDAGRR PAVRLGLREI
     SGFPEAAALR VAEARAAAPF ADTNDLARRA ALQRRELDLL AAAGALQALA GHRRQAAWQV
     AGVCLQGDLF DAAPPPEAAV ALAAPQEAEE LLADYAATGF SLGRHPLALL RARLARGRFL
     QAAALARTPD RALVRVAGIV TGRQRPGTAQ GVIFVTLEDE TGSANVVVYA GLAERQRREL
     LGARLLGVFG QLQREGEVVH LLAKRLVDLS PWVGGLAARS RDFH