ID   DNAE2_BORBR             Reviewed;        1052 AA.
AC   Q7WHW8;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=BB3088;
OS   Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50)
OS   (Alcaligenes bronchisepticus).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Bordetella.
OX   NCBI_TaxID=257310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-588 / NCTC 13252 / RB50;
RX   PubMed=12910271; DOI=10.1038/ng1227;
RA   Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R.,
RA   Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L.,
RA   Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A.,
RA   Achtman M., Atkin R., Baker S., Basham D., Bason N., Cherevach I.,
RA   Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T.,
RA   Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S.,
RA   Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E.,
RA   Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M.,
RA   Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S.,
RA   Barrell B.G., Maskell D.J.;
RT   "Comparative analysis of the genome sequences of Bordetella pertussis,
RT   Bordetella parapertussis and Bordetella bronchiseptica.";
RL   Nat. Genet. 35:32-40(2003).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; BX640446; CAE33580.1; -; Genomic_DNA.
DR   RefSeq; WP_010926731.1; NC_002927.3.
DR   AlphaFoldDB; Q7WHW8; -.
DR   SMR; Q7WHW8; -.
DR   STRING; 257310.BB3088; -.
DR   EnsemblBacteria; CAE33580; CAE33580; BB3088.
DR   GeneID; 66439193; -.
DR   KEGG; bbr:BB3088; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_4; -.
DR   OMA; HVEAREQ; -.
DR   OrthoDB; 561611at2; -.
DR   Proteomes; UP000001027; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1052
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103367"
SQ   SEQUENCE   1052 AA;  115105 MW;  D95FADC2CA5D656F CRC64;
     MSAILPGYAE LHCQSNFSFL QGASHPEELV TRAGELGYAA LALTDECSLA GVVRAHVEAR
     EQKLPLIIGS SFTLQAGADA PPLDLTLLAQ NREGYGNLAE LITLGRGRAA KGQYLLTPAD
     IEAPAGDNAH LRGMPDCLAI LAPPPGLAAE RLAEQARWLA AQCPGRAWIG LTLLHHCRDD
     LHRAAVEHAA HASGLPIVAL GQAQMHRRSR KPLHDTLAAI RTGRSVGQCG YDLAANAERH
     LRSRLRLASL YPAQALAQTL AIARRCTFSL DELQYEYPDE IVPAGHTPAS YLRQQTYLGA
     RQRFPEGMTP AVAAQVEKEL ALINELRYEA YFLTVYDIVG YARSQGILCQ GRGSAANSAV
     CYCLGITAVD PARGNTLFER FISKERNEPP DIDVDFEHQR REEVIQYIYE KYGRQRAALT
     AVVISYRPRS VLRDTGRALG VDNGIIDAVA RAHQWWDGKK EMLRSLAACG LDPASRVARQ
     WAELAETLMG FPRHLSQHPG GFVISRGKLS RLVPIENAAM PGRSVVQWDK DDLDALRLLK
     VDVLALGMLS VLRRALALAG QRRGRPLALH EIPPDDDATY DMICAADTIG VFQIESRAQM
     SMLPRLRPRQ YYDLVVQVAI VRPGPIQGGM VHPYLRRRQG REDITYPGPA VRKALARTLG
     VPIFQEQVMQ IAVDAAGFTP GEADALRRSM AAWRRKGGVD KFRAQLVGGL LARNYTADFA
     QALFRQIEGF GEYGFPESHA ASFALLAYAS SWLKCHEPEA FLAALLNSQP MGFYAPAQLV
     QDARRHGVRV LPADVLYSGW EASLQDAPGA ARPAVRLGLN LVKGLREDSA RAIEQARVRR
     PFADTADMAR RAGLPRQALD ALAAADALRT LAGHRRLASW QAAASAQSRD LLREAVIVET
     ETPALPAPSE GQTVAADYRS VGLTLGRHPL ALLRPQLAAR NFQTAAVLNT YPNRRLARAC
     GIVTVRQRPQ TAKGTIFVTL EDETGPINAV VRPELIERQR RELLDATLLG IYGTWQSVDG
     VRHLVAQRLV DLSSLLGQLS QDGLAAASRN FH