ID   DNAE2_BRUME             Reviewed;        1077 AA.
AC   Q8YEK2;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 2.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=BMEI1876;
OS   Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=224914;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=16M / ATCC 23456 / NCTC 10094;
RX   PubMed=11756688; DOI=10.1073/pnas.221575398;
RA   DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA   Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA   Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA   Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA   Haselkorn R., Kyrpides N.C., Overbeek R.;
RT   "The genome sequence of the facultative intracellular pathogen Brucella
RT   melitensis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL53057.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE008917; AAL53057.1; ALT_INIT; Genomic_DNA.
DR   PIR; AF3486; AF3486.
DR   RefSeq; WP_004684632.1; NZ_GG703778.1.
DR   AlphaFoldDB; Q8YEK2; -.
DR   SMR; Q8YEK2; -.
DR   STRING; 224914.BMEI1876; -.
DR   EnsemblBacteria; AAL53057; AAL53057; BMEI1876.
DR   GeneID; 29594750; -.
DR   KEGG; bme:BMEI1876; -.
DR   PATRIC; fig|224914.52.peg.1701; -.
DR   eggNOG; COG0587; Bacteria.
DR   OMA; NSWPMGF; -.
DR   PhylomeDB; Q8YEK2; -.
DR   Proteomes; UP000000419; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1077
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103371"
SQ   SEQUENCE   1077 AA;  120982 MW;  1B13F29D15C68B26 CRC64;
     MVPYFEMAAA SNFSFLCGAS HPQELVERAH ALDLSGIGIA DRNTLAGVVR AHAQWKDIRK
     ESGFRLFIGC RLSFIDGTPD MVVYPRDRAA YGQLCRLLTE GKHRAAIKGE CHLEWADLLF
     RARQFQIAVF PPDEDEPDFA ARLTEIAQAA PGSVWLALTM PHQGQDGRRA ERIARFAAQA
     GVPLIATNDV LYHHPDRRPL QDVLTATRHH TTVFAAGRLL EKNAERHLKP PHEMVRLFRD
     YPEAIAATAD FVAPITFQLD ELKYAYPDEP IPPGKTAQQH LYDLVWEGAA RHYGADMIPP
     KVQGLINKEL ALIARLEYEP YFLTVYDIVT HAREKGILCQ GRGSAANSVV CFCLGITGVN
     PTQVDLLFER FISAERKEPP DIDVDFEHER REEVMQYVYD RYSRDRAAIV ATVISYRSRS
     AIRDVGKALG LSEDVTAALA NTVWGLSGGG IDRQHIRQAG LDPDNPIIQR AVELAITLIG
     FPRHLSQHVG GFVLTRDRLD ETVPIGPAAM DKRSFIEWDK DDIDEVGLMK VDVLSLGMLT
     CIRKAFDLIH QHKPQLYGGE KLTLASLPRK DKAVYDMLCK GDSLGVFQVE SRAQMNMLPR
     LRPQEFYDLV IEVAIVRPGP IQGDMVHPYL RRRSGQEPCT LPSPSPQHGP ANELQQILGK
     TKGVPLFQEQ AMRIAMEAAK FTPEEANQLR RAMATFRKMG TIHTMEKKMI DGMVNRGYDR
     TFAENCFNQI KGFGEYGFPE SHAASFAHLV YISAWLKCHH PEVFAAALLN SQPMGFYAPA
     QIVRDAREHG VTVLPVDVNF SQWDNILEET PDVHLALRLG FRQIDGFSKR DTELLIADRQ
     EPYRTIEDMH RRLRLDRRAF TLLADADAFG SLDIDRRAAL WAVRRLPNDE TLLLFRAAAA
     SELAQEPRTK LPEMAASEHV IADYETTRLS LKGHPLQYLR EGLAAEGVST CRAVQEGADG
     RRMKVAGVVT VRQRPGSAKG VVFLTIEDET GIANIVIWPK IMKVFRREVM SARLIHIEGR
     IQRSLEGVVH LVAAKLQDRS AALIEMSGRE AQRLIAPSQM AHHPRNVRVM PNSRDFH