DNAE2_BURMA
ID DNAE2_BURMA Reviewed; 1063 AA.
AC Q62F99;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=BMA3145;
OS Burkholderia mallei (strain ATCC 23344).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia; pseudomallei group.
OX NCBI_TaxID=243160;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 23344;
RX PubMed=15377793; DOI=10.1073/pnas.0403306101;
RA Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E.,
RA Feldblyum T.V., Ulrich R.L., Ronning C.M., Brinkac L.M., Daugherty S.C.,
RA Davidsen T.D., DeBoy R.T., Dimitrov G., Dodson R.J., Durkin A.S.,
RA Gwinn M.L., Haft D.H., Khouri H.M., Kolonay J.F., Madupu R., Mohammoud Y.,
RA Nelson W.C., Radune D., Romero C.M., Sarria S., Selengut J., Shamblin C.,
RA Sullivan S.A., White O., Yu Y., Zafar N., Zhou L., Fraser C.M.;
RT "Structural flexibility in the Burkholderia mallei genome.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:14246-14251(2004).
CC -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC translesion synthesis (TLS). It is not the major replicative DNA
CC polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01902};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR EMBL; CP000010; AAU48350.1; -; Genomic_DNA.
DR RefSeq; WP_004195295.1; NC_006348.1.
DR RefSeq; YP_104628.1; NC_006348.1.
DR AlphaFoldDB; Q62F99; -.
DR SMR; Q62F99; -.
DR STRING; 243160.BMA3145; -.
DR PRIDE; Q62F99; -.
DR EnsemblBacteria; AAU48350; AAU48350; BMA3145.
DR GeneID; 56593837; -.
DR KEGG; bma:BMA3145; -.
DR PATRIC; fig|243160.12.peg.3223; -.
DR eggNOG; COG0587; Bacteria.
DR HOGENOM; CLU_001600_4_0_4; -.
DR OMA; NSWPMGF; -.
DR Proteomes; UP000006693; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR HAMAP; MF_01902; DNApol_error_prone; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR023073; DNA_pol_error_prone.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR004805; PolC_alpha.
DR PANTHER; PTHR32294; PTHR32294; 1.
DR PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; SSF89550; 1.
DR TIGRFAMs; TIGR00594; polc; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA damage; DNA repair; DNA replication;
KW DNA-directed DNA polymerase; Nucleotidyltransferase; Transferase.
FT CHAIN 1..1063
FT /note="Error-prone DNA polymerase"
FT /id="PRO_0000103373"
SQ SEQUENCE 1063 AA; 117255 MW; 297605302D2629F4 CRC64;
MDAASGILPD YAELFCRSNF SFLHGASSAE ELVERAAKQG YRGIAITDEC SLAGAPRMHV
AAKAVGLPLV VGAYFGVTPD DAAPGHDPGP GAFGLVLLAQ NREGYGNLSE LISWRRMNAP
KGTYRLTPRM LAAPPRALAH LRGVPDCFAI LVPTYPARAD VLDAQLAWFD ALFGERARLG
LVQLQRALDG AHREQVRAAG ERRGMHIVAL GDVTMHIRSC KPLQDTMTAI RLGMPIAECG
HALAPNGEQH LRTRQRIAQL FPADALAQTC RMLDACHFSL DDLRYEYPHE IVPAGHTPTS
YLAQETWAGA RRRYPDGVPD TVRQRIEFEL ALIADLKYEP YFLTVYDIVK YARSKDILCQ
GRGSAANSVV CYCLGVTEVN PQQSTLLFER FLSRERGEPP DIDVDFEHQR REEVIQYLYE
KYGHDRAALA AAVSTYRPRG ALRETGKALG VDPMLVERVA KEHRWFDGSR DLLARFASVG
LDPEVPLIRT WAEIAARLLN FPRHLSQHSG GFVVSRGKLT RLVPVENAAM EGRRVIQWDK
DDLEALGLMK VDVLALGMLS ALHRAFDMIT AWRGPPLPDG RPFRLEHIPQ DDEATYDMIC
RADTVGVFQI ESRAQMSMLP RLRPRGYYDL VVQVSIVRPG PIQGGAVHPY LERRRIAAGE
AHGEITYPSE ALERVLERTL GIPIFQEQVM QIAIVAAGFT PGEADALRRA MAAWKRKGDL
GKYHERIVAG MLERGYSREF AEQIFEQIKG FGEYGFPESH AASFAKLAYA SSWLKRHEPA
IFLAALLNSQ PMGFYPPAQL VQDAKRHGVT VLPIDATKSG WEASLEAQPG AAPPDGRPAV
RLGLSLVRGL GEEAARRIGA ARAAGPFASV DELARRACLE RRDLEALAAA NAFATLAGNR
RDALWQAVAA APIDEAVRPA LGAPTEADDV FADYRTIGLT LNRHPVALLR PALDARRLSS
AAALRDRRNG RLARACGLVT ARQMPGTAKG VLFVTLEDET GCVNVIVRPE LLERQRRETL
DSQLLAVSGV WQCESDVRHL VAQYLEDLTP LIAGLRTESR EFH