ID   DNAE2_BURMA             Reviewed;        1063 AA.
AC   Q62F99;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=BMA3145;
OS   Burkholderia mallei (strain ATCC 23344).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; pseudomallei group.
OX   NCBI_TaxID=243160;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23344;
RX   PubMed=15377793; DOI=10.1073/pnas.0403306101;
RA   Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E.,
RA   Feldblyum T.V., Ulrich R.L., Ronning C.M., Brinkac L.M., Daugherty S.C.,
RA   Davidsen T.D., DeBoy R.T., Dimitrov G., Dodson R.J., Durkin A.S.,
RA   Gwinn M.L., Haft D.H., Khouri H.M., Kolonay J.F., Madupu R., Mohammoud Y.,
RA   Nelson W.C., Radune D., Romero C.M., Sarria S., Selengut J., Shamblin C.,
RA   Sullivan S.A., White O., Yu Y., Zafar N., Zhou L., Fraser C.M.;
RT   "Structural flexibility in the Burkholderia mallei genome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:14246-14251(2004).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; CP000010; AAU48350.1; -; Genomic_DNA.
DR   RefSeq; WP_004195295.1; NC_006348.1.
DR   RefSeq; YP_104628.1; NC_006348.1.
DR   AlphaFoldDB; Q62F99; -.
DR   SMR; Q62F99; -.
DR   STRING; 243160.BMA3145; -.
DR   PRIDE; Q62F99; -.
DR   EnsemblBacteria; AAU48350; AAU48350; BMA3145.
DR   GeneID; 56593837; -.
DR   KEGG; bma:BMA3145; -.
DR   PATRIC; fig|243160.12.peg.3223; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_4; -.
DR   OMA; NSWPMGF; -.
DR   Proteomes; UP000006693; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1063
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103373"
SQ   SEQUENCE   1063 AA;  117255 MW;  297605302D2629F4 CRC64;
     MDAASGILPD YAELFCRSNF SFLHGASSAE ELVERAAKQG YRGIAITDEC SLAGAPRMHV
     AAKAVGLPLV VGAYFGVTPD DAAPGHDPGP GAFGLVLLAQ NREGYGNLSE LISWRRMNAP
     KGTYRLTPRM LAAPPRALAH LRGVPDCFAI LVPTYPARAD VLDAQLAWFD ALFGERARLG
     LVQLQRALDG AHREQVRAAG ERRGMHIVAL GDVTMHIRSC KPLQDTMTAI RLGMPIAECG
     HALAPNGEQH LRTRQRIAQL FPADALAQTC RMLDACHFSL DDLRYEYPHE IVPAGHTPTS
     YLAQETWAGA RRRYPDGVPD TVRQRIEFEL ALIADLKYEP YFLTVYDIVK YARSKDILCQ
     GRGSAANSVV CYCLGVTEVN PQQSTLLFER FLSRERGEPP DIDVDFEHQR REEVIQYLYE
     KYGHDRAALA AAVSTYRPRG ALRETGKALG VDPMLVERVA KEHRWFDGSR DLLARFASVG
     LDPEVPLIRT WAEIAARLLN FPRHLSQHSG GFVVSRGKLT RLVPVENAAM EGRRVIQWDK
     DDLEALGLMK VDVLALGMLS ALHRAFDMIT AWRGPPLPDG RPFRLEHIPQ DDEATYDMIC
     RADTVGVFQI ESRAQMSMLP RLRPRGYYDL VVQVSIVRPG PIQGGAVHPY LERRRIAAGE
     AHGEITYPSE ALERVLERTL GIPIFQEQVM QIAIVAAGFT PGEADALRRA MAAWKRKGDL
     GKYHERIVAG MLERGYSREF AEQIFEQIKG FGEYGFPESH AASFAKLAYA SSWLKRHEPA
     IFLAALLNSQ PMGFYPPAQL VQDAKRHGVT VLPIDATKSG WEASLEAQPG AAPPDGRPAV
     RLGLSLVRGL GEEAARRIGA ARAAGPFASV DELARRACLE RRDLEALAAA NAFATLAGNR
     RDALWQAVAA APIDEAVRPA LGAPTEADDV FADYRTIGLT LNRHPVALLR PALDARRLSS
     AAALRDRRNG RLARACGLVT ARQMPGTAKG VLFVTLEDET GCVNVIVRPE LLERQRRETL
     DSQLLAVSGV WQCESDVRHL VAQYLEDLTP LIAGLRTESR EFH