ID   DNAE2_BURPS             Reviewed;        1072 AA.
AC   Q63XR8;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=BPSL0472;
OS   Burkholderia pseudomallei (strain K96243).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; pseudomallei group.
OX   NCBI_TaxID=272560;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K96243;
RX   PubMed=15377794; DOI=10.1073/pnas.0403302101;
RA   Holden M.T.G., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.-M.,
RA   Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K.L., Bentley S.D.,
RA   Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A.,
RA   Brown N.F., Challis G.L., Cherevach I., Chillingworth T., Cronin A.,
RA   Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z.,
RA   Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S.,
RA   Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M.,
RA   Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M.,
RA   Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J.;
RT   "Genomic plasticity of the causative agent of melioidosis, Burkholderia
RT   pseudomallei.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:14240-14245(2004).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; BX571965; CAH34461.1; -; Genomic_DNA.
DR   RefSeq; WP_004543942.1; NZ_CP009538.1.
DR   RefSeq; YP_107097.1; NC_006350.1.
DR   AlphaFoldDB; Q63XR8; -.
DR   SMR; Q63XR8; -.
DR   STRING; 272560.BPSL0472; -.
DR   EnsemblBacteria; CAH34461; CAH34461; BPSL0472.
DR   KEGG; bps:BPSL0472; -.
DR   PATRIC; fig|272560.51.peg.1183; -.
DR   eggNOG; COG0587; Bacteria.
DR   OMA; NSWPMGF; -.
DR   Proteomes; UP000000605; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1072
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103374"
SQ   SEQUENCE   1072 AA;  118134 MW;  C627677138FFFF65 CRC64;
     MDAASGILPD YAELFCRSNF SFLHGASSAE ELVERAAKQG YRGIAITDEC SLAGAPRMHV
     AAKAVGLPLV VGAYFGVTPD DAAPGHDPGP GAFGLVLLAQ NREGYGNLSE LISWRRMNAP
     KGTYRLTPRM LAAPPRALAH LRGVPDCFAI LVPTYPARAD VLDAQLAWFD ALFGERARLG
     LVQLQRALDG AHREQVRAAG ERRGMHIVAL GDVTMHIRSC KPLQDTMTAI RLGMPIAECG
     HALAPNGEQH LRTRQRIAQL FPADALAQTC RMLDACHFSL DDLRYEYPHE IVPAGHTPTS
     YLAQETWAGA RRRYPDGVPD TVRQRIEFEL ALIADLKYEP YFLTVYDIVK YARSKDILCQ
     GRGSAANSVV CYCLGVTEVN PQQSTLLFER FLSRERGEPP DIDVDFEHQR REEVIQYLYE
     KYGHDRAALA AAVSTYRPRG ALRETGKALG VDPMLVERVA KEHRWFDGSR DLLARFASVG
     LDPEVPLIRT WAEIAARLLN FPRHLSQHSG GFVVSRGKLT RLVPVENAAM EGRRVIQWDK
     DDLEALGLMK VDVLALGMLS ALHRAFDMIT AWRGPPLPDG RPFRLEHIPQ DDEATYDMIC
     RADTVGVFQI ESRAQMSMLP RLRPRGYYDL VVQVSIVRPG PIQGGAVHPY LERRRIAAGE
     AHGEITYPSE ALERVLERTL GIPIFQEQVM QIAIVAAGFT PGEADALRRA MAAWKRKGDL
     GKYHERIVAG MLERGYSREF AEQIFEQIKG FGEYGFPESH AASFAKLAYA SSWLKRHEPA
     IFLAALLNSQ PMGFYPPAQL VQDAKRHGVT VLPIDATKSG WEASLEAQPG AAPPDGRPAV
     RLGLSLVRGL GEEAARRIGA ARAAGPFASV DELARRACLE RRDLEALAAA NAFATLAGNR
     RDALWQAVAA APERGLLAAA PIDEAVRPAL GAPTEADDVF ADYRTIGLTL NRHPVALLRP
     ALDARRLSSA AALRDRRNGR LARACGLVTA RQMPGTAKGV LFVTLEDETG CVNVIVRPEL
     LERQRRETLD SQLLAVSGVW QCESDVRHLV AQYLEDLTPL IAGLRTESRE FH