ID   DNAE2_CAUVN             Reviewed;        1083 AA.
AC   B8H427;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Error-prone DNA polymerase;
DE            EC=2.7.7.7;
GN   Name=dnaE2; OrderedLocusNames=CCNA_03317;
OS   Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC   Caulobacteraceae; Caulobacter.
OX   NCBI_TaxID=565050;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NA1000 / CB15N;
RX   PubMed=20472802; DOI=10.1128/jb.00255-10;
RA   Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V.,
RA   Walunas T.L., Crosson S.;
RT   "The genetic basis of laboratory adaptation in Caulobacter crescentus.";
RL   J. Bacteriol. 192:3678-3688(2010).
RN   [2]
RP   FUNCTION.
RX   PubMed=15886391; DOI=10.1093/nar/gki551;
RA   Galhardo R.S., Rocha R.P., Marques M.V., Menck C.F.M.;
RT   "An SOS-regulated operon involved in damage-inducible mutagenesis in
RT   Caulobacter crescentus.";
RL   Nucleic Acids Res. 33:2603-2614(2005).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). Along with ImuA and ImuB is required for
CC       the error-prone processing of DNA lesions.
CC       {ECO:0000269|PubMed:15886391}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: Up-regulated in response to DNA damage in a RecA-dependent
CC       manner.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000305}.
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DR   EMBL; CP001340; ACL96781.1; -; Genomic_DNA.
DR   RefSeq; WP_010921044.1; NC_011916.1.
DR   RefSeq; YP_002518689.1; NC_011916.1.
DR   AlphaFoldDB; B8H427; -.
DR   SMR; B8H427; -.
DR   EnsemblBacteria; ACL96781; ACL96781; CCNA_03317.
DR   GeneID; 7330340; -.
DR   KEGG; ccs:CCNA_03317; -.
DR   PATRIC; fig|565050.3.peg.3234; -.
DR   HOGENOM; CLU_001600_4_0_5; -.
DR   OMA; NSWPMGF; -.
DR   OrthoDB; 561611at2; -.
DR   PhylomeDB; B8H427; -.
DR   Proteomes; UP000001364; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1083
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000378287"
SQ   SEQUENCE   1083 AA;  120800 MW;  124E5F216A555E64 CRC64;
     MRPPVYAELQ ATTNFSFLRG ASHAEELALT AEALGLTAIG IVDRNSLAGV VRAWTAAKRR
     SIRVLTGCRL DFMDGAPSLL CYPTDREAFG RLTRLLTIGQ LRAEKGECHL TWRDFLDHSE
     GQLGLIVPPR VLDDSFEQHL TRMAGDLRGR SWLAASRAYA ARDLQRLARL ESLGRTSGAP
     IVATNDVLYH GPERRPLQDV ISCVREHCAI QEAGFRLEAN AERHIKSPEE MARLFDRWPR
     AVERTVEIVE RIGFDLKDIR EQYPDEPVPP GKTAMQHLTD LTWKGAAWRY PNGVSPKVTA
     QIQEELRLIE KMDYPNYFIT VHDIVREARS MGILCQGRGS AANSSVCFCL GVTAIDPTEH
     RLLFTRFISE NRGEPPDIDV DFEHDRREEV MQYVFQRYGR AYAAICGTVI HYRPRSAIRD
     VGKALGLTED VTSLLAGTVW GSWGDGLPED HLRNAGLDPK APEIARAVGL ANDLIGFPRH
     LSQHVGGFVL TKRRLDETVP IGKAAMKDRT FIEWDKDDID SLGLMKVDIL ALGMLHAIQR
     AMTMLREDHG QDWLKDLADI PKEVPGVYDM LCAADSVGVF QVESRAQMSM LPRLRPREFY
     DLVIQVAIVR PGPIQGDMVH PYLKRRNKEE PVDWPKPSPE HGPPDELQEI LGKTFGVPLF
     QEQAMSLAIE AAKFTPDEAD GLRKAMATFR NLGSPDAYRN KFIEGMVGRG YERAFAERCF
     KQIEGFSHYG FPESHAASFA KLVYVSAWIK WAWPDVFCAA LINAQPMGFY QPAQLVRDAR
     EHGVEVLPPD ILTSDWDCTL APISTGFRPP RVRHDKVACQ ETRPRWKAVR LGFRQIKGLR
     ETLDIPPLLK ARGEGARTPA EFAQGGVPQK ALELLAEADA FASVGLSRRE ALWAVKGLKG
     EHKAPVQAPL LAGLPLFEER VALPAMAATQ EVAEDYRTTS LSLKAHPIGF YRSMLAARGV
     VPAERLLSLK DGARVSVAGL VLIRQRPGTA KGVVFVTLED ETGVANAVVW KDRFDAARNV
     VMTASFLIVH GRVQRADNVI HVVAERFTDL SAELSSLRDE PGAPAPRIRQ KVSGRLLRSR
     DFH