ID   DNAE2_CORDI             Reviewed;        1039 AA.
AC   Q6NJ04;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=DIP0612;
OS   Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype
OS   gravis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC   Corynebacterium.
OX   NCBI_TaxID=257309;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700971 / NCTC 13129 / Biotype gravis;
RX   PubMed=14602910; DOI=10.1093/nar/gkg874;
RA   Cerdeno-Tarraga A.-M., Efstratiou A., Dover L.G., Holden M.T.G.,
RA   Pallen M.J., Bentley S.D., Besra G.S., Churcher C.M., James K.D.,
RA   De Zoysa A., Chillingworth T., Cronin A., Dowd L., Feltwell T., Hamlin N.,
RA   Holroyd S., Jagels K., Moule S., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Thomson N.R., Unwin L., Whitehead S., Barrell B.G.,
RA   Parkhill J.;
RT   "The complete genome sequence and analysis of Corynebacterium diphtheriae
RT   NCTC13129.";
RL   Nucleic Acids Res. 31:6516-6523(2003).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BX248355; CAE49129.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q6NJ04; -.
DR   SMR; Q6NJ04; -.
DR   STRING; 257309.DIP0612; -.
DR   PRIDE; Q6NJ04; -.
DR   EnsemblBacteria; CAE49129; CAE49129; DIP0612.
DR   KEGG; cdi:DIP0612; -.
DR   HOGENOM; CLU_001600_4_0_11; -.
DR   OMA; NSWPMGF; -.
DR   Proteomes; UP000002198; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1039
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103376"
SQ   SEQUENCE   1039 AA;  113416 MW;  B0978740FB847B14 CRC64;
     MVDWVSGVNG KPLKWSQLEG VLSGTYPQEF KLANVGGSGS GSEKIRYYSS QPFAELHAAS
     YYHFLHGANS PQDMVAAACQ QGMSALALID RDGFYGAMEF AEAAATSGLP TVFGAELTLA
     PDRILTVLAR GVEGYTSLSR LITQARMRGS KDVTIYPPLA EIVESMGKNC RFLLNHSWCT
     DESLLEIFYP DTVVLEYGVT MRPEDVDHHE LLDSIRQRYG FMAIASAVPS AVDRVSARVA
     GAKQALAQRE SLPQATPRLH PMGSTWMRGG DELAGSVDKQ LLDASVEVAR DCAFALDLVA
     PNLPRWPVPD EMAHLTGLVN ARFDARYRGR SEDVKNQARA QIHHELAVIK ELGFPGYFLI
     VDDIVSFCHT NNILCQGRGS AANSVVCFVL GITNVEPIST GLLFERFLSR DREGPPDIDI
     DIESGRRDAV ITYVYDTYGR DNAAQVANVI TYRTKAALRD AARALGYPNT SADAWSTNPD
     EAPNAVRFLA EQLKGQPRHL GIHAGGMVIC DRPIADVVPT EWARKHGRSV VQWDKESCAS
     AGLVKFDLLG LGMLEALHHM IDLVVETTGT TINLWEIDLA EPEIYTMLSN GDAVGVFQVE
     SRAQLATLPR LRPREFFDLV VEVALVRPGP IQGGSVHPYI RRRNGLEAVT YEHPILERSL
     RKTLGVPLFQ EQLMHIAVDA AGFSGGEADE LRRAMGSRRS VDTMNRMKRR FFAGLRRNNI
     TGDIAGILWN KIVAFVAYGF PESHSQSFAS LVYFSAWFKY HHPAEFYVGL LRAQPMGFYS
     PQSLLADARR KELTILSHNI NKSQVEATVE NGAIRLGLNM IKGVGKTEAE AIVAHAPYRD
     IADLSRRAGL GVAVIEALAR SGALETLGVG RRQALWQAGA AATEKAAMLP GLSMVSAPSL
     PGMNAFELIV ADIAMTGVSS ENPVALLRRQ LELRGVIPAS ELLNCENARR ILLAGVVTHR
     QRPQTAAGVT FLGLEDETGL ANVVVSVGLW KRSRAVKVAR AVLVRGVVHN ADGVASVTAD
     RVEELPFAEM MSAGSRDFR