DNAE2_CORGL
ID DNAE2_CORGL Reviewed; 1055 AA.
AC Q6M7C6; Q8NSN0;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902};
GN OrderedLocusNames=Cgl0638, cg0738;
OS Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 /
OS JCM 1318 / LMG 3730 / NCIMB 10025).
OC Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC Corynebacterium.
OX NCBI_TaxID=196627;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=12743753; DOI=10.1007/s00253-003-1328-1;
RA Ikeda M., Nakagawa S.;
RT "The Corynebacterium glutamicum genome: features and impacts on
RT biotechnological processes.";
RL Appl. Microbiol. Biotechnol. 62:99-109(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=12948626; DOI=10.1016/s0168-1656(03)00154-8;
RA Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A.,
RA Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A.,
RA Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F.,
RA Moeckel B., Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O.,
RA Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.;
RT "The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its
RT impact on the production of L-aspartate-derived amino acids and vitamins.";
RL J. Biotechnol. 104:5-25(2003).
CC -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC translesion synthesis (TLS). It is not the major replicative DNA
CC polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01902};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB98031.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BA000036; BAB98031.1; ALT_INIT; Genomic_DNA.
DR EMBL; BX927149; CAF19345.1; -; Genomic_DNA.
DR RefSeq; NP_599872.1; NC_003450.3.
DR RefSeq; WP_011013785.1; NC_006958.1.
DR AlphaFoldDB; Q6M7C6; -.
DR SMR; Q6M7C6; -.
DR STRING; 196627.cg0738; -.
DR PRIDE; Q6M7C6; -.
DR KEGG; cgb:cg0738; -.
DR KEGG; cgl:Cgl0638; -.
DR PATRIC; fig|196627.13.peg.624; -.
DR eggNOG; COG0587; Bacteria.
DR HOGENOM; CLU_001600_4_0_11; -.
DR OMA; NSWPMGF; -.
DR Proteomes; UP000000582; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0009432; P:SOS response; EXP:CollecTF.
DR HAMAP; MF_01902; DNApol_error_prone; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR023073; DNA_pol_error_prone.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR004805; PolC_alpha.
DR PANTHER; PTHR32294; PTHR32294; 1.
DR PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 2.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; SSF89550; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA damage; DNA repair; DNA replication;
KW DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..1055
FT /note="Error-prone DNA polymerase"
FT /id="PRO_0000103378"
SQ SEQUENCE 1055 AA; 115099 MW; 91B4912894FEB6C2 CRC64;
MYPIIMEWNG GGSFNGRPLS WSKLERILSG KKVESLRPVL HEPDAQAPSS AMQGEASVPF
AELHATSSYN FLTGASDPSD VVVQAKKLGL VALSVMDRDG FYGAVRFAEA AAEAGMHTVY
GAELSLQEGV LTVLCKNPEG YKKLSHLISD AKMATGEKGE VRYPPLPMVA EHAAGDWVVL
AGFQWLDKID YVIDCFKPEN IVLEFGSTMT PEDADRNEYL RRTQAKFQLR GILSTNPESA
ARGSVRLAGA KQALARKMPL ADAESELHPM GTTWMRSGDT LLKAHPDYAD LIATTVELAA
ECAFTLDLVA PNLPKWDTPG EHTEMSWLAH LVSTRIDTRY VGRSADIKAR AATQIDYELG
VIEKLGFPGY FLVVNDLVEF CRDSNILCQG RGSAANSAVC FVLGITNAEP ISAGLLFERF
LSPDRDGPPD IDIDIESGRR EEVIQYVYEK YGRDNAAQVA NVITYRTKGA MRDAARALGY
PQGAADAWAK GTSEPPDDVL ELAAQFKGQP RHLGIHSGGM VICDRPIADV VPVEWARMDN
RSVVQWDKDD CATAGLVKFD LLGLGMLEAI HHMLDLVAEH RGKKINLWEL DLAEPEVYDM
LCKADAVGVF QVESRAQLST LPRLKPRTFF DLVVEVALIR PGPIQGGSVH PYLRRRAGEE
AITYDHPVLE KSLGKTLGIP LFQEQLMQVA VDAAGFSGGE ADSLRRAMGS KRSPERMAAL
RSRFFQGLKD TNGIVGETAE KLWNKIVAFA AYGFPESHSQ SFASLVYFSA WFKYHYPAEF
CVGLLRAQPM GFYSPQSLIS DARRHGVSIL PITVNDSGVE ADAPNGAIRL GLNLVKGLGH
DAAQRIEDNA PFDSIPDLSR RADLNVAQVE ALARAGAVDC LGVGRRQALW QAGVAATEKP
GMLPGLSVIE APALPGMSAF ELMATNISAT GVTADYQPMA LIRERMEELG IVPADRLLEV
EDGTRLRIAG IVTHRQRPQT ASGLTFLGME DETGLMNVMV SVGLWQRQRV LARNAKALII
RGIVQNAQGV ATVVADRLEP LDMGEFLSRG SRDFR
