ID   DNAE2_DECAR             Reviewed;        1027 AA.
AC   Q47EP3;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=Daro_1942;
OS   Dechloromonas aromatica (strain RCB).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Azonexaceae;
OC   Dechloromonas.
OX   NCBI_TaxID=159087;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RCB;
RX   PubMed=19650930; DOI=10.1186/1471-2164-10-351;
RA   Salinero K.K., Keller K., Feil W.S., Feil H., Trong S., Di Bartolo G.,
RA   Lapidus A.;
RT   "Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB:
RT   indications of a surprisingly complex life-style and cryptic anaerobic
RT   pathways for aromatic degradation.";
RL   BMC Genomics 10:351-351(2009).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; CP000089; AAZ46688.1; -; Genomic_DNA.
DR   RefSeq; WP_011287690.1; NC_007298.1.
DR   AlphaFoldDB; Q47EP3; -.
DR   SMR; Q47EP3; -.
DR   STRING; 159087.Daro_1942; -.
DR   EnsemblBacteria; AAZ46688; AAZ46688; Daro_1942.
DR   KEGG; dar:Daro_1942; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_4; -.
DR   OMA; VAKSHHW; -.
DR   OrthoDB; 561611at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1027
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_1000070590"
SQ   SEQUENCE   1027 AA;  113395 MW;  C83D577695B12DCF CRC64;
     MSLPEYAELH CLSNFSFLRG ASHPEELAAR ALAQGYAALA LTDECSLAGV VRAHLAAKKH
     GLKFIVGSEM MTVDGLKLVF LACNRHGYGN LSALITLARR RAEKGGYTLH RNDLESISPS
     GALPDCLVLW VPGNNPSSAD GEWLVSRFPG RSWIAVELHA GPEDAERLAG LQSLGEVCGL
     PLVATGDVHM HIKARRPVQD VLTALRLKST VFEAGYALFP NGERHLRSRL RLSRLYPPEL
     LAETLNIAAR CEFLLDELRY EYPEEIIPSG ETPASWLRSE TERGLQRRYP AGVPGSVRER
     IEHELGLIAE MAYEAYFLTV YDIVCYARSQ DILCQGRGSA ANSAVCYALG VTEVDPARSA
     LLFERFVSKE RGEPPDIDVD FEHERREDVI QYIYTKYGRE RAALAAALIT YRTKGALRDA
     GRALGFGIAQ INALTASLAW WDKREQLPER FAELGLDPHA PRVEKWLAIA EALRGFPRHL
     TQHVGGFVIS RGPLSRLVPV ENAAMSARSV IQWDKDDLDA MGLMKVDILA LGMLSAIRRM
     LQIVGETTGR PMKMQDIPAE DPATYEMLCH ADSMGVFQVE SRAQMAMLPR LRPQNFYDLV
     VEVALVRPGP IQGDMVHPYL KRRQGRERIE EISPAVDAVL ERTYGVPIFQ EQVMQLAVVA
     ANFTPGEADQ LRRAMAAWKR KGGLEPFEQK LLAGMAANDL PESFARRIIA QIQGFGEYGF
     PESHAASFAL LVYASAWLKR HHPAAFLCGL LNSQPMGFYS PSMLIQDARR HGVRVLPPDV
     MTSDWDSRLD ERGAVRLGLR EISGFSVAAA KRITAVCREN QPFLNVADLA ARAGLQRRDL
     DLLAAGDALQ GLAGHRRQAA WAATVAVVQG DLFDGTPVVE AEIELPAPSD GENLVADYRS
     LGLTLRSHPL SLLRQYLAER RFVTAADLKM AGHHTLIRSV GIVVGRQRPG TATGIVFVTL
     EDETGLSNVV VHPQLVEKQR RELLGSTLLG VYGQLQVEGE VVHLVAKRLV DLSAWLGRLE
     TVSRDFH