ID   DNAE2_IDILO             Reviewed;        1039 AA.
AC   Q5QUT9;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=IL2566;
OS   Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Idiomarinaceae; Idiomarina.
OX   NCBI_TaxID=283942;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-735 / DSM 15497 / L2-TR;
RX   PubMed=15596722; DOI=10.1073/pnas.0407638102;
RA   Hou S., Saw J.H., Lee K.S., Freitas T.A., Belisle C., Kawarabayasi Y.,
RA   Donachie S.P., Pikina A., Galperin M.Y., Koonin E.V., Makarova K.S.,
RA   Omelchenko M.V., Sorokin A., Wolf Y.I., Li Q.X., Keum Y.S., Campbell S.,
RA   Denery J., Aizawa S., Shibata S., Malahoff A., Alam M.;
RT   "Genome sequence of the deep-sea gamma-proteobacterium Idiomarina
RT   loihiensis reveals amino acid fermentation as a source of carbon and
RT   energy.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:18036-18041(2004).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE017340; AAV83398.1; -; Genomic_DNA.
DR   RefSeq; WP_011235789.1; NC_006512.1.
DR   AlphaFoldDB; Q5QUT9; -.
DR   SMR; Q5QUT9; -.
DR   STRING; 283942.IL2566; -.
DR   EnsemblBacteria; AAV83398; AAV83398; IL2566.
DR   KEGG; ilo:IL2566; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_6; -.
DR   OMA; NSWPMGF; -.
DR   OrthoDB; 561611at2; -.
DR   Proteomes; UP000001171; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1039
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103381"
SQ   SEQUENCE   1039 AA;  117525 MW;  FE225ED52A3CE332 CRC64;
     MAGSADISKG CSGQLFAELH SLSHYSFLRS AASPEELVRR ADDLGYAAIA ITDECSVAGI
     VKAYQESKAR DIKLVVGSEF RVPEVGTLVV LAPTREAYAE LCKKITLFRS RSEKGHYQAN
     IDDFFDGFSQ CLLLWQPDFS QAQLLAIGEK LKSVWQQRFW LLYERHYQAD DERAFQAMVE
     LQQAVKVKVT CAGGVCMATI EQQPLYDILN AIQHGGSLAH QPGLASNNAE HHLRSLSELQ
     QCYPQKWLAE TLVIAQQCQF CLSELRYEYP AELVPEGMSA SGYLKQLTEQ GARWRYPQGV
     PESIKALYLK ELSLIKEQSY EPFFLTIHDL VRFAREQGIL FQGRGSAANS VVCYCLGITA
     VNPAQIQVLF ERFISKERNE PPDIDVDFEH ERREEVIQYI YRKYGRQRAA LAATVITYRF
     KSAFRDVGKA LGFSEQQLQY FRRNLDRRDK EQSWQQQLVQ QHPEVTNSVR GQHLLQFTEQ
     LMGTPRHLSQ HVGGFVIAAD DLSRLVPVEN ASMTDRTVIQ WDKTDLESLG LLKVDVLALG
     MLTALRKMLA LINLRYDQQL TMATIPQEDS RVYQQLQTAD SMGIFQIESR AQMNMLPRLK
     PKTFYDLVIQ IAIVRPGPIQ GDMVHPFLRR RAGLEEVDYP SDEVKSVLER TLGVPIFQEQ
     VIKLAMVAAG FSGGEADQLR RAMASWGQNG HLTRFEDKLI NGMLERGYKQ EFAERLFRQI
     CGFGQYGFPE SHSASFANLA YVSSWFKTYH PAAFYVGLLN SLPMGFYSAS QLVQDAQRHG
     VAFLAADINA SDWNYCWLPK EDTNSDETVR MGLRQIQGLS ESRIKHTLMQ RPEDGFTSMH
     ELRKSGLRDS DINALARADA LKSIAGHRHQ AHWQSLSMSD QQMPLFENVK DRPARQLPAP
     DEPDNIKADY ESTGLTLGRH PLALLRNNPE LKNCVLASDL MTCRSGQVLT LAGLVTCRQR
     PGTRSGVTFL TLEDESGNSN VVVWANTARQ FRQCFLTSNL LWVKGIVEIH DGVVHVIAGR
     LKDLSGLLSF TRLESRRFH