ID   DNAE2_METCA             Reviewed;        1047 AA.
AC   Q605W1;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=MCA2164;
OS   Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales;
OC   Methylococcaceae; Methylococcus.
OX   NCBI_TaxID=243233;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33009 / NCIMB 11132 / Bath;
RX   PubMed=15383840; DOI=10.1371/journal.pbio.0020303;
RA   Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S.,
RA   Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E.,
RA   Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J.,
RA   Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L.,
RA   Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H.,
RA   Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R.,
RA   Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A.;
RT   "Genomic insights into methanotrophy: the complete genome sequence of
RT   Methylococcus capsulatus (Bath).";
RL   PLoS Biol. 2:1616-1628(2004).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; AE017282; AAU91620.1; -; Genomic_DNA.
DR   RefSeq; WP_010961400.1; NC_002977.6.
DR   AlphaFoldDB; Q605W1; -.
DR   SMR; Q605W1; -.
DR   STRING; 243233.MCA2164; -.
DR   EnsemblBacteria; AAU91620; AAU91620; MCA2164.
DR   KEGG; mca:MCA2164; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_6; -.
DR   OMA; NSWPMGF; -.
DR   OrthoDB; 561611at2; -.
DR   Proteomes; UP000006821; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1600; -; 1.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1047
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103382"
SQ   SEQUENCE   1047 AA;  116728 MW;  6CA4ACD601B82A94 CRC64;
     MACSAEAVFA ELHALSNFSF LRGASHPKEL VAEAARLGYA ALALTDECSL AGVVRAHAEA
     KTLGFKLIIG SEFRLEDGPK LVLLAMDRRG YGQLAAFITL GRRAAEKGEY RLLRGQLETH
     PLDQCIAIWL PEDAPDDETG AWLARLFPAR CWIGVELFLS GGDGLRLSVL QALGERLGLP
     LVACNDVHMH ARERQPLQDT LTAIRLGRPL AELGYALFPN GERHLRPIEF LARIYPRALL
     EESLRIAERC RFSLDELRYE YPAELVPDGY TAIAWLRELT RQGMAERWPE GAPDKVRRQV
     EHELELIGAM AYEPFFLTVH DVVRFARSRG ILCQGRGSAA NSAVCYCLGI TEVDPARLDL
     LFERFISRER NEPPDIDVDF EHERREEVIQ YIYRKYGRHR AALAASLITY RVRSAVRDVA
     RALGFSPSRI DTLARVLDRH GVAETLPERL AEAGLASENP AVRRLLALMQ MLVGFPRHLS
     QHVGGFVIAA EDLSHWVPVE NAAMPERTVI QWDKDDLESL GLLKVDVLSL GMLTAIRKAL
     AYVGEGRGRP FTLADVPPED PAVYEMLQRA DSIGVFQVES RAQMSMLPRL RPQSYYDLVI
     QIAIVRPGPI QGDMVHPYLT RRAGLEPVSY PSPAVEKVLK RTLGVPIFQE QVMQLAMVAA
     GFTPGEADRL RRAMAAWHRK GGLEPFEKKL MDGMRERGYE ERFARQIYRQ IRGFGEYGFP
     ESHSASFALL AYVSAWLKCH HPAAFACALL NSQPMGFYGP SQLIQDARRH GVEVRPIDVN
     HSDWDCTLEP TEIPNPLSLW ERVGVRALQT GSNPALRLGL RLVNGFSSAA AQRLSDARRQ
     GPFQSIQDLA TRVPLDRREL EALAAADALH GLGGHRHRAF WEAAGVEAPT PLYAEPQFAE
     AEPLLRKPGE AEDVIADYAA AGASLRRHPL SLLRERLDRR GFRTAEALWQ VRNGAIARVA
     GLVVCRQRPM TANGTTFVTI EDETGQINLI VWPATAQAQR RALLRAHLLA VSGTVQQEEG
     VLHLVAGRLE DIGKWLDGLV VKSRDFQ