ID   DNAE2_MYCBO             Reviewed;        1091 AA.
AC   Q7TWL9; A0A1R3Y411; X2BN20;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 2.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   OrderedLocusNames=BQ2027_MB3405C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=SIU02034.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; LT708304; SIU02034.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_857046.2; NC_002945.3.
DR   RefSeq; WP_003417885.1; NC_002945.4.
DR   AlphaFoldDB; Q7TWL9; -.
DR   SMR; Q7TWL9; -.
DR   EnsemblBacteria; SIU02034; SIU02034; BQ2027_MB3405C.
DR   PATRIC; fig|233413.5.peg.3740; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1091
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103383"
FT   REGION          1..51
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1091 AA;  117784 MW;  F4973A856D56711D CRC64;
     MGWSNGPPSW AEMERVLNGK PRHAGVPAFD ADGDVPRSRK RGAYQPPGRE RVGSSVAYAE
     LHAHSAYSFL DGASTPEELV EEAARLGLCA LALTDHDGLY GAVRFAEAAA ELDVRTVFGA
     ELSLGATART ERPDPPGPHL LVLARGPEGY RRLSRQLAAA HLAGGEKGKP RYDFDALTEA
     AGGHWHILTG CRKGHVRQAL SQGGPAAAQR ALADLVDRFT PSRVSIELTH HGHPLDDERN
     AALAGLAPRF GVGIVATTGA HFADPSRGRL AMAMAAIRAR RSLDSAAGWL APLGGAHLRS
     GEEMARLFAW CPEAVTAAAE LGERCAFGLQ LIAPRLPPFD VPDGHTEDSW LRSLVMAGAR
     ERYGPPKSAP RAYSQIEHEL KVIAQLRFPG YFLVVHDITR FCRDNDILCQ GRGSAANSAV
     CYALGVTAVD PVANELLFER FLSPARDGPP DIDIDIESDQ REKVIQYVYH KYGRDYAAQV
     ANVITYRGRS AVRDMARALG FSPGQQDAWS KQVSHWTGQA DDVDGIPEQV IDLATQIRNL
     PRHLGIHSGG MVICDRPIAD VCPVEWARMA NRSVLQWDKD DCAAIGLVKF DLLGLGMLSA
     LHYAKDLVAE HKGIEVDLAR LDLSEPAVYE MLARADSVGV FQVESRAQMA TLPRLKPRVF
     YDLVVEVALI RPGPIQGGSV HPYIRRRNGV DPVIYEHPSM APALRKTLGV PLFQEQLMQL
     AVDCAGFSAA EADQLRRAMG SKRSTERMRR LRGRFYDGMR ALHGAPDEVI DRIYEKLEAF
     ANFGFPESHA LSFASLVFYS AWFKLHHPAA FCAALLRAQP MGFYSPQSLV ADARRHGVAV
     HGPCVNASLA HATCENAGTE VRLGLGAVRY LGAELAEKLV AERTANGPFT SLPDLTSRVQ
     LSVPQVEALA TAGALGCFGM SRREALWAAG AAATGRPDRL PGVGSSSHIP ALPGMSELEL
     AAADVWATGV SPDSYPTQFL RADLDAMGVL PAERLGSVSD GDRVLIAGAV THRQRPATAQ
     GVTFINLEDE TGMVNVLCTP GVWARHRKLA HTAPALLIRG QVQNASGAIT VVAERMGRLT
     LAVGARSRDF R