ID   DNAE2_MYCTO             Reviewed;        1098 AA.
AC   P9WNT4; L0TF55; O50399; Q7D5L9;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Error-prone DNA polymerase;
DE            EC=2.7.7.7;
GN   Name=dnaE2; OrderedLocusNames=MT3480;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE000516; AAK47817.1; -; Genomic_DNA.
DR   PIR; A70972; A70972.
DR   AlphaFoldDB; P9WNT4; -.
DR   SMR; P9WNT4; -.
DR   EnsemblBacteria; AAK47817; AAK47817; MT3480.
DR   KEGG; mtc:MT3480; -.
DR   HOGENOM; CLU_001600_4_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1098
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000427071"
FT   REGION          34..57
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1098 AA;  118672 MW;  F07709ACDBF35E88 CRC64;
     MFDILWNVGW SNGPPSWAEM ERVLNGKPRH AGVPAFDADG DVPRSRKRGA YQPPGRERVG
     SSVAYAELHA HSAYSFLDGA STPEELVEEA ARLGLCALAL TDHDGLYGAV RFAEAAAELD
     VRTVFGAELS LGATARTERP DPPGPHLLVL ARGPEGYRRL SRQLAAAHLA GGEKGKPRYD
     FDALTEAAGG HWHILTGCRK GHVRQALSQG GPAAAQRALA DLVDRFTPSR VSIELTHHGH
     PLDDERNAAL AGLAPRFGVG IVATTGAHFA DPSRGRLAMA MAAIRARRSL DSAAGWLAPL
     GGAHLRSGEE MARLFAWCPE AVTAAAELGE RCAFGLQLIA PRLPPFDVPD GHTEDSWLRS
     LVMAGARERY GPPKSAPRAY SQIEHELKVI AQLRFPGYFL VVHDITRFCR DNDILCQGRG
     SAANSAVCYA LGVTAVDPVA NELLFERFLS PARDGPPDID IDIESDQREK VIQYVYHKYG
     RDYAAQVANV ITYRGRSAVR DMARALGFSP GQQDAWSKQV SHWTGQADDV DGIPEQVIDL
     ATQIRNLPRH LGIHSGGMVI CDRPIADVCP VEWARMANRS VLQWDKDDCA AIGLVKFDLL
     GLGMLSALHY AKDLVAEHKG IEVDLARLDL SEPAVYEMLA RADSVGVFQV ESRAQMATLP
     RLKPRVFYDL VVEVALIRPG PIQGGSVHPY IRRRNGVDPV IYEHPSMAPA LRKTLGVPLF
     QEQLMQLAVD CAGFSAAEAD QLRRAMGSKR STERMRRLRG RFYDGMRALH GAPDEVIDRI
     YEKLEAFANF GFPESHALSF ASLVFYSAWF KLHHPAAFCA ALLRAQPMGF YSPQSLVADA
     RRHGVAVHGP CVNASLAHAT CENAGTEVRL GLGAVRYLGA ELAEKLVAER TANGPFTSLP
     DLTSRVQLSV PQVEALATAG ALGCFGMSRR EALWAAGAAA TGRPDRLPGV GSSSHIPALP
     GMSELELAAA DVWATGVSPD SYPTQFLRAD LDAMGVLPAE RLGSVSDGDR VLIAGAVTHR
     QRPATAQGVT FINLEDETGM VNVLCTPGVW ARHRKLAHTA PALLIRGQVQ NASGAITVVA
     ERMGRLTLAV GARSRDFR