ID   DNAE2_MYCTU             Reviewed;        1098 AA.
AC   P9WNT5; L0TF55; O50399; Q7D5L9;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 40.
DE   RecName: Full=Error-prone DNA polymerase;
DE            EC=2.7.7.7;
GN   Name=dnaE2; OrderedLocusNames=Rv3370c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   INDUCTION BY LEXA.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=12029045; DOI=10.1128/jb.184.12.3287-3295.2002;
RA   Davis E.O., Dullaghan E.M., Rand L.;
RT   "Definition of the mycobacterial SOS box and use to identify LexA-regulated
RT   genes in Mycobacterium tuberculosis.";
RL   J. Bacteriol. 184:3287-3295(2002).
RN   [3]
RP   FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=12705867; DOI=10.1016/s0092-8674(03)00270-8;
RA   Boshoff H.I.M., Reed M.B., Barry C.E. III, Mizrahi V.;
RT   "DnaE2 polymerase contributes to in vivo survival and the emergence of drug
RT   resistance in Mycobacterium tuberculosis.";
RL   Cell 113:183-193(2003).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. Does not appear to be essential for chromosomal
CC       replication. May be involved in generating antibiotic resistance.
CC       {ECO:0000269|PubMed:12705867}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: Up-regulated in response to all forms of DNA damage. LexA-
CC       regulated. {ECO:0000269|PubMed:12029045}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CCP46191.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AL123456; CCP46191.1; ALT_INIT; Genomic_DNA.
DR   PIR; A70972; A70972.
DR   RefSeq; NP_217887.3; NC_000962.3.
DR   AlphaFoldDB; P9WNT5; -.
DR   SMR; P9WNT5; -.
DR   IntAct; P9WNT5; 1.
DR   STRING; 83332.Rv3370c; -.
DR   PaxDb; P9WNT5; -.
DR   GeneID; 887259; -.
DR   KEGG; mtu:Rv3370c; -.
DR   PATRIC; fig|83332.12.peg.3764; -.
DR   TubercuList; Rv3370c; -.
DR   eggNOG; COG0587; Bacteria.
DR   OMA; NSWPMGF; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IDA:MTBBASE.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0042276; P:error-prone translesion synthesis; IDA:MTBBASE.
DR   GO; GO:0046677; P:response to antibiotic; IEP:MTBBASE.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1098
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103385"
FT   REGION          34..57
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1098 AA;  118672 MW;  F07709ACDBF35E88 CRC64;
     MFDILWNVGW SNGPPSWAEM ERVLNGKPRH AGVPAFDADG DVPRSRKRGA YQPPGRERVG
     SSVAYAELHA HSAYSFLDGA STPEELVEEA ARLGLCALAL TDHDGLYGAV RFAEAAAELD
     VRTVFGAELS LGATARTERP DPPGPHLLVL ARGPEGYRRL SRQLAAAHLA GGEKGKPRYD
     FDALTEAAGG HWHILTGCRK GHVRQALSQG GPAAAQRALA DLVDRFTPSR VSIELTHHGH
     PLDDERNAAL AGLAPRFGVG IVATTGAHFA DPSRGRLAMA MAAIRARRSL DSAAGWLAPL
     GGAHLRSGEE MARLFAWCPE AVTAAAELGE RCAFGLQLIA PRLPPFDVPD GHTEDSWLRS
     LVMAGARERY GPPKSAPRAY SQIEHELKVI AQLRFPGYFL VVHDITRFCR DNDILCQGRG
     SAANSAVCYA LGVTAVDPVA NELLFERFLS PARDGPPDID IDIESDQREK VIQYVYHKYG
     RDYAAQVANV ITYRGRSAVR DMARALGFSP GQQDAWSKQV SHWTGQADDV DGIPEQVIDL
     ATQIRNLPRH LGIHSGGMVI CDRPIADVCP VEWARMANRS VLQWDKDDCA AIGLVKFDLL
     GLGMLSALHY AKDLVAEHKG IEVDLARLDL SEPAVYEMLA RADSVGVFQV ESRAQMATLP
     RLKPRVFYDL VVEVALIRPG PIQGGSVHPY IRRRNGVDPV IYEHPSMAPA LRKTLGVPLF
     QEQLMQLAVD CAGFSAAEAD QLRRAMGSKR STERMRRLRG RFYDGMRALH GAPDEVIDRI
     YEKLEAFANF GFPESHALSF ASLVFYSAWF KLHHPAAFCA ALLRAQPMGF YSPQSLVADA
     RRHGVAVHGP CVNASLAHAT CENAGTEVRL GLGAVRYLGA ELAEKLVAER TANGPFTSLP
     DLTSRVQLSV PQVEALATAG ALGCFGMSRR EALWAAGAAA TGRPDRLPGV GSSSHIPALP
     GMSELELAAA DVWATGVSPD SYPTQFLRAD LDAMGVLPAE RLGSVSDGDR VLIAGAVTHR
     QRPATAQGVT FINLEDETGM VNVLCTPGVW ARHRKLAHTA PALLIRGQVQ NASGAITVVA
     ERMGRLTLAV GARSRDFR