ID   DNAE2_NOCFA             Reviewed;        1146 AA.
AC   Q5Z1D1;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=NFA_9150;
OS   Nocardia farcinica (strain IFM 10152).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Nocardia.
OX   NCBI_TaxID=247156;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IFM 10152;
RX   PubMed=15466710; DOI=10.1073/pnas.0406410101;
RA   Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K.,
RA   Shiba T., Hattori M.;
RT   "The complete genomic sequence of Nocardia farcinica IFM 10152.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; AP006618; BAD55760.1; -; Genomic_DNA.
DR   RefSeq; WP_011207445.1; NC_006361.1.
DR   AlphaFoldDB; Q5Z1D1; -.
DR   SMR; Q5Z1D1; -.
DR   STRING; 247156.NFA_9150; -.
DR   PRIDE; Q5Z1D1; -.
DR   EnsemblBacteria; BAD55760; BAD55760; NFA_9150.
DR   KEGG; nfa:NFA_9150; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_11; -.
DR   OMA; NSWPMGF; -.
DR   Proteomes; UP000006820; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1146
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103386"
FT   REGION          1..43
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          154..178
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1146 AA;  123632 MW;  D02899A853403CEF CRC64;
     MGWGTGPPSW SELERVLSGR PGRTDPEAMY PGDGGDSPAW SRKREPYLAE PIRPVDRPTV
     PYAELHAHSA YSFLDGASQP EELVEEAVRL GLEAIALTDH DGFYGTVRFA EAAKEWGIAT
     VFGAELSLGV ARPAPRRSAR KAARGRGRAV RYAATPEFEH AAAPDSAPRT GEPDPPGPHL
     LVLARGQEGY RRLSREIAAA HLAAGEKGVL RYDIDALTAA AGGHWHILTG CRKGHLRTAL
     ADDLAAGETG LPRAEAALRD LVERFGADRV SVELTHHGVP ADDERNALLI ALADRVGLPV
     LATTGAHFAG PEQRRRAMAL AAIRARRSLD EMAGWLAPAG GAHLRSGAEM ARLFAACPDA
     VANAVALSRE CAFDLRLIAP QLPPFAVPDG HDENSWLREL VLRGAARRYG PPHANPPAYR
     QLEHELEVIA TLGFPGYFLV VHDIVSFCER NGILCQGRGS AANSAVCFAI GITNVDPVAN
     ELLFERFLSP ERDGPPDIDI DIESDRREEA IQHVYARYGR EYAAQVANVI TYRGKSAVRD
     AAKALGFSPG QQDAWSKQVS RWTGVGAETG TDIPEQVLRL AADLEGLPRH MGIHSGGMVI
     CDRPIADVCP VEWARMAGRS VLQWDKDDCA AVGLVKFDML GLGMLSALHY MIDLVREHEG
     VEVRLHELDL KEPAVYEMLS RADSVGVFQV ESRAQMATLP RLRPREFYDL VVEVALIRPG
     PIQGGSVHPY IRRRNGTEPV TYDHPALENS LGRTLGVPLF QEQLMQMAVD VAGFTAAEAD
     QLRRAMGSKR SPERMRRLKE RLYRGMAELH GITGEVADRI YEKLYAFANF GFPESHSQSF
     AALVFYSAWF KLHHPAAFCA GLLRAQPMGF YSPQSLVADA RRHGVPVHGP DVNASLAEAT
     LENGGREVRL GLAGVRHIGS ELAERIVAAR VEHGPYTSVL DLTGRVELTV AQAEALATAG
     AFDSVTGPSG AERSGGRRAA LWAAGAAARE RAHLLPGTGP AADAPALPGM SALELAAADV
     WATGISPGSY PTEFLRARMD ELGVIPAGRL LEVRDGSRVL VGGAVTHRQR PATAAGVTFL
     NLEDETGMVN VVCSVGLWTR YRALAQSASA LLVRGRVQNA EGAVSVVAEH LQLLSLGMGS
     RSRDFR