ID   DNAE2_PSEAE             Reviewed;        1031 AA.
AC   Q9I5Q2;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=PA0669;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; AE004091; AAG04058.1; -; Genomic_DNA.
DR   PIR; F83561; F83561.
DR   RefSeq; NP_249360.3; NC_002516.2.
DR   RefSeq; WP_010895514.1; NZ_QZGE01000025.1.
DR   AlphaFoldDB; Q9I5Q2; -.
DR   SMR; Q9I5Q2; -.
DR   STRING; 287.DR97_1450; -.
DR   PaxDb; Q9I5Q2; -.
DR   PRIDE; Q9I5Q2; -.
DR   EnsemblBacteria; AAG04058; AAG04058; PA0669.
DR   GeneID; 880686; -.
DR   KEGG; pae:PA0669; -.
DR   PATRIC; fig|208964.12.peg.700; -.
DR   PseudoCAP; PA0669; -.
DR   HOGENOM; CLU_001600_4_0_6; -.
DR   InParanoid; Q9I5Q2; -.
DR   OMA; NSWPMGF; -.
DR   PhylomeDB; Q9I5Q2; -.
DR   BioCyc; PAER208964:G1FZ6-679-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1031
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103388"
SQ   SEQUENCE   1031 AA;  115769 MW;  06C6EB22D9F4AD40 CRC64;
     MAAWLVRMSA YAELHCLSNF SFQRGASSAT ELFARAARLG YRALAITDEC SLAGIVRAWQ
     AAREHQVQLI VGSEIRLEQG PKLVLLAEDL EGYQNLCRLI TRGRRQADKG HYRLLREDLQ
     QPLAGLLAIW LPNDHGDEQA AWLRERFPQR LWLGVELHRG ADDDARLDKL LALASHLRLP
     PVACGDVHMH ARGRRALQDC MTAIRNHLPV SEAGAYLFPN GERHLRTLEA LQGIYPQALL
     AETLKIAERC RFDLEQLKYQ YPRELVPAGH DPASWLRHLT EQGIARRWPN GASAKVRKQI
     ERELELIAEL GYESYFLTVQ DIVAFARGRK ILCQGRGSAA NSAVCFALGI TELDPDRTNL
     LFERFLSRER NEPPDIDVDF EHERREEVIQ YVFTRYGRQR AALTAVVSTY HGAGAVRDVA
     KALGLPPEQV DVLANCCGRW SDQAPSAERL EEAGFDPQSP ILRRVLALTD ELIGFPRHLS
     QHPGGFVISE QPLDTLVPVE NASMAERTVI QWDKDDLDAV GLLKVDVLAL GMLSALRRSF
     DLIHALRGGK RLSIASIPSE DPATYEMISR ADTIGVFQIE SRAQMAMLPR LRPQKFYDLV
     IQVAIVRPGP IQGDMVHPYL RRRNGEEPVA YPSAELEEVF ERTLGVPLFQ EQVMELAIVA
     ADYTPGEADE LRRSMAAWKR HGGLEHHRER LTRGMLAKGY EADFAARIFE QIKGFGSYGF
     PESHAASFAL LTYASSWLKR HEPAAFACAL INSWPMGFYS PDQLLQDARR HALQTRPVDV
     RHSGWDCSLE SFGQAQPAIR LGLRMIRGFR EEDARRIEQV REAEPFLDVH DLGRRARLDA
     RALELLADAG ALRGLAGHRH KARWAVASVE PQLPLFAEGT AIEESTVSLP LPSRGEELLS
     DYALLGTTLG PHPLKLLRGQ LKARRCRDSR ELAKLGHGRP IRVAGLVVGR QRPQTASGIT
     FITLEDEFGM VNVVVRHDLA ERQRRPFLES RLLQVEGILE SSGEVRHVIA GRLHDLTPLL
     TGLDVRSRDF H