ID   DNAE2_PSEF5             Reviewed;        1034 AA.
AC   Q4KAD4;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=PFL_3697;
OS   Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=220664;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-477 / NRRL B-23932 / Pf-5;
RX   PubMed=15980861; DOI=10.1038/nbt1110;
RA   Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A.,
RA   Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J.,
RA   Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J.,
RA   Gwinn M.L., Zhou L., Schneider D.J., Cartinhour S.W., Nelson W.C.,
RA   Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III,
RA   Thomashow L.S., Loper J.E.;
RT   "Complete genome sequence of the plant commensal Pseudomonas fluorescens
RT   Pf-5.";
RL   Nat. Biotechnol. 23:873-878(2005).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; CP000076; AAY92963.1; -; Genomic_DNA.
DR   RefSeq; WP_011061972.1; NC_004129.6.
DR   AlphaFoldDB; Q4KAD4; -.
DR   SMR; Q4KAD4; -.
DR   STRING; 220664.PFL_3697; -.
DR   EnsemblBacteria; AAY92963; AAY92963; PFL_3697.
DR   KEGG; pfl:PFL_3697; -.
DR   PATRIC; fig|220664.5.peg.3788; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_6; -.
DR   OMA; NSWPMGF; -.
DR   OrthoDB; 561611at2; -.
DR   Proteomes; UP000008540; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1034
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103389"
SQ   SEQUENCE   1034 AA;  115233 MW;  21B064CB3642664D CRC64;
     MSAYAELHCL SNFSFQRGAS SAAELFQRAK ALGYQALAIT DECTLAGIVR AWQASRDTGL
     KLIVGSEMQV EDGPRLVLLV EDLAGYQGLC RLITRARRRA EKGSYRLLRE DFAEPLPGLL
     ALWLTGAGEP LAQGRWLQQV FPQRLWLALE LHCGQDDARH LQQQLALARQ LGLPAVACGD
     VHMHVRGRRA LQDTMTAIRH HLPVAEAGQR LFANGERHLR PVEVLQGLYP QALLDESLAI
     AGRCAFDLGQ LRYQYPRELV PEGHDAGSWL RVLTQRGIVR RWPTGARAEV LKQIDKELTL
     ICDLGYESYF LTVHDIVDFA RRQHILCQGR GSAANSVVCF ALGITEIDPD RSTLLFERFL
     SRERNEPPDI DVDFEHERRE EVLQYLFQRY GRHRAALTAV VSTYHGAGAV RDVAKALGLP
     PDQVNALADC CGHWSDTPPS VERLQEAGFD PQSPVLRRVL SLTGQLIGFP RHLSQHPGGF
     VISEQPLDHL VPVENATMAE RTVIQWDKDD LDMVGLLKVD ILALGMLSAI RRCFDLIHGY
     RGQRYSLASI PPEDPATYEM IGRADTIGVF QIESRAQMSM LPRLKPQNFY DLVIEVAIVR
     PGPIQGGMVH PYLRRRNRQE PETYPSPELE GVLKRTLGVP LFQEQVMQIA IVAADYTPGE
     ADQLRRSMAA WKRHGGLEPH RERLRRGMNR NGYSDEFATQ IFEQIKGFGS YGFPESHAAS
     FALLTYASCW LKCHEPAAFA CALINSWPMG FYSPDQILQD ARRHGLQVLA VDVGASDWDC
     SLEPIPVQPA RAGNGPPGQP ALRLGLRMIK GFREDDARRI ERARRQRAFV DIADLGERAR
     LDVRAQELLA DAGALQALAG DRYRARWEVA GVERQLGLFA GLPSQEEAPV ALPRPSVGED
     LQADYHSLGT TLGPHPLALL RPQLAARRCR SSRDLLAVEH GRSVSVAGLV TGRQRPGTAS
     GVTFVTLEDE FGNVNVVVWR DLAERQRRVL VGAQLLKVDG TLETEGEVRH LIAGRLSDLS
     PLLDGIHVRS RDFR