ID   DNAE2_PSEPF             Reviewed;        1025 AA.
AC   Q3KBG7;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=Pfl01_3149;
OS   Pseudomonas fluorescens (strain Pf0-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=205922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pf0-1;
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; CP000094; ABA74887.1; -; Genomic_DNA.
DR   RefSeq; WP_011334532.1; NC_007492.2.
DR   AlphaFoldDB; Q3KBG7; -.
DR   SMR; Q3KBG7; -.
DR   STRING; 205922.Pfl01_3149; -.
DR   EnsemblBacteria; ABA74887; ABA74887; Pfl01_3149.
DR   KEGG; pfo:Pfl01_3149; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_6; -.
DR   OMA; NSWPMGF; -.
DR   Proteomes; UP000002704; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1025
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_1000070594"
SQ   SEQUENCE   1025 AA;  115238 MW;  B1C2F0E4DB44A93F CRC64;
     MNQGYAELHC LSNFSFQRGA SSALELFQRA KKHGYQALAI TDECTLAGIV RAWQAAKSVE
     LPLIIGSEIR IENGPKLVLL VENIEGYQAL CGLITQARRR TQKGQYQILR EDFSEPLPGL
     LVLWVPEAVD EVEEGRWLKQ TFGERLWLAV QLHRGQNDQQ RLAALLSLAD ELQIPAVASG
     DVHMHARGRR ALQDTMTAIR HHVPVAEAGL RLHPNGERHL RSLDVLRELY PQTLLDESLK
     LARRCTFDLG ELRYQYPKEL VPEEHSASSW LRHLTEQGIA WRWPKGAQPK VLKQIDDELE
     LIAELGYESY FLTVHDVVRF AREQKILCQG RGSAANSAVC FALGITEIDP DRTTLLFERF
     MSRERNEPPD IDVDFEHERR EEVLQYVFRR YGRRRAALTA VVSTYHASGA IRDVAKALGL
     PPDQINALAD CCGHWSDETP PVERLREGGF DPESPLLHRV LSLTGQLIGF PRHLSQHPGG
     FVISEQPLDT LVPVENAAMA DRTIIQWDKD DLDAVGLLKV DILALGMLSA IRRCFDLLRR
     HRHQDLSLAT IPPEDRPTYD MISRADTIGV FQIESRAQMS MLPRLRPQTF YDLVIEVAIV
     RPGPIQGGMV HPYLRRRNKE EEETYPSPEL EVVLKRTLGV PLFQEQVMQI AIVAADYSPG
     EADQLRRSMA AWKRHGGLEP HKERLAAGMK KNGYSPEFAA QIFEQIKGFG SYGFPESHAA
     SFALLTYASC WLKCHEPAAF ACALINSWPM GFYSPDQILQ DARRHHLQIR PVDVRASDWD
     CSLEPIAGEQ PAIRMGLRMI KGFREEDARS IEKARARGAF ADVADLGERA GLDSRAQALL
     ADAGALRGLA GHRHRARWEV AGVQKQLGLF AGLPSQEEPD VLLPTPSVSE DLFTDYATLG
     TTLGPHPLTL LRNELRARRC RSSRDLLEVE HGRPVSVAGL VTGRQRPGTA SGVTFVTLED
     EFGNVNVVVW RDLAERQRQV LVGSQLLKVD GRWEREGEVR HLIAGRLSDL TPLLNGIRVQ
     SRDFH