ID   DNAE2_PSEPK             Reviewed;        1033 AA.
AC   Q88I82;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=PP_3119;
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; AE015451; AAN68727.1; -; Genomic_DNA.
DR   RefSeq; NP_745263.3; NC_002947.4.
DR   AlphaFoldDB; Q88I82; -.
DR   SMR; Q88I82; -.
DR   STRING; 160488.PP_3119; -.
DR   EnsemblBacteria; AAN68727; AAN68727; PP_3119.
DR   KEGG; ppu:PP_3119; -.
DR   PATRIC; fig|160488.4.peg.3310; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_6; -.
DR   OMA; NSWPMGF; -.
DR   PhylomeDB; Q88I82; -.
DR   BioCyc; PPUT160488:G1G01-3335-MON; -.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1033
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103390"
SQ   SEQUENCE   1033 AA;  115631 MW;  63F6929BED45D995 CRC64;
     MAAGLVRMNT PGYAELHCLS NFSFQRGASS ADELFRRARE QGYQALAITD ECTLAGIVRA
     WQAAKVHQLR LIVGSEVQLC DGPKLVLLVE NLTGYQNLCA LITRARRRAE KGAYQLFRDD
     LLLHHQGLLA LWVAADSGDT ATGAWLRSVF AERLWLAVHL HRGSDDAVRL QRLRALAADV
     GIRAVACGDV HMHVRGRRAL QDCMTAIRQH CQVSEAGRFL FANGERHLRS QAQLAELYPL
     DLLAETLVIA RRCQFDLSEL NYQYPRELVP EGHTPASWLR ELCEQGMPLR WPDGPSGKVR
     DVLAKELGLI EELGYESYFL TVHDIVAFAR SQRILCQGRG SAANSVVCFV LGITELDPMK
     HHLLFERFLS RERNEPPDID VDFEHDRREE VIQYVFRRYG RHRAALTAVV NTYHAAGAVR
     DVARALGLPA DQVDALAKCC GRWSDRIPDD QRLAEAGFEA GSPSLRRVLV LAGELIGFPR
     HLSQHPGGFV ISQQPLDQLV PVENAAMPER TVIQWDKDDL DMVGLLKVDV LALGMLSALR
     RCFDQLQHHR GRHLTLATIP SEDPATYAMI SRAETMGVFQ IESRAQMAML PRLRPQKFYD
     LVIEVAIVRP GPIQGDMVHP YLRRRLKQEP VTYPSPQLKE VFERTLGVPL FQEQVMELAM
     VAADYTPGEA DQLRRSMAAW KRHGGLEPHR ERLVQGMLRN GYTLAFAERI FEQIKGFGSY
     GFPESHAASF ALLCYASSWL KCHEPAIFTC ALVNSWPMGF YSPDQLLQEA RRQGIEVRPV
     DVCHSDWDCT LEPDAEGTLA IRMGLRLVRG LAEADAKRVQ QARSQRPWRN VEDLCLRAGL
     DARARARLAD GGALRALASD RHQARWQVAA VQPQLPLFAD VQALPEEPVQ LPVPTVGEDL
     MADYQTLGTT LGPHPLALLR ARLRALGCRS SSELQGVEHG DNIAVAGLVV GRQRPQTASG
     VTFVTLEDEH GMVNVVVWRA LAERQRRALV GSQLLKVSGR LEQENGVRHL IARRLEDVSP
     LLQGLDVRSR DFH