ID   DNAE2_PSESM             Reviewed;        1031 AA.
AC   Q881T7;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=PSPTO_2795;
OS   Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=223283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RX   PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA   Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA   Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA   Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA   Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA   Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA   Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA   Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA   Collmer A.;
RT   "The complete genome sequence of the Arabidopsis and tomato pathogen
RT   Pseudomonas syringae pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; AE016853; AAO56295.1; -; Genomic_DNA.
DR   RefSeq; NP_792600.1; NC_004578.1.
DR   RefSeq; WP_011104197.1; NC_004578.1.
DR   AlphaFoldDB; Q881T7; -.
DR   SMR; Q881T7; -.
DR   STRING; 223283.PSPTO_2795; -.
DR   PRIDE; Q881T7; -.
DR   EnsemblBacteria; AAO56295; AAO56295; PSPTO_2795.
DR   GeneID; 1184449; -.
DR   KEGG; pst:PSPTO_2795; -.
DR   PATRIC; fig|223283.9.peg.2853; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_6; -.
DR   OMA; NSWPMGF; -.
DR   OrthoDB; 561611at2; -.
DR   PhylomeDB; Q881T7; -.
DR   Proteomes; UP000002515; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; ISS:JCVI.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; ISS:JCVI.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1031
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103392"
SQ   SEQUENCE   1031 AA;  115990 MW;  146574D77567B9D9 CRC64;
     MNTEYAELHC LSNFSFQRGA SSARELFDRA LRHGYAALAI TDECTLAGIV RAWQASKDTG
     LPLIVGSEMH LENGPKIVLL VENLTGYQAL CTLITVARRR AKKGEYRLLL EDFDHLPGGL
     LAIWLADIEG DAQACLAQGN WLRERFAERL WLGVELHRGA DDEQRLADLL ALAQSLAIPA
     VASGDVHMHA RGRRALQDTI TAIRHHTTVA EAGHLLFANG ERHLRSLDAL AEHYPDWLLA
     ESLRIARRCT FTFDQLQYEY PHELVPKGQT STSWLRELTE RGIRRRWPRG LSVAARAQVE
     KELALITEKK FDSYFLTVHD IVEFARSQHI LCQGRGSAAN SAVCYALGIT ELNPEKSNLL
     FERFISKERD EPPDIDVDFE HDRREEVIQY IFRRYGRGRA ALTAVASTYH GSGALRDVAK
     VLGLPPDQIN ALADAFSRWS DSLPSPERLR EYGFDAETPI LKRVLALTGE LIGFPRHLSQ
     HPGGFVISEH PLDTLVPVEN AAMAERTIIQ WDKDDLDLVG LLKVDILALG MLSALRRTFD
     LVHLHRGKQW TLASLPGDDR PTYEMISRAD TIGVFQIESR AQMAMLPRLR PEKFYDLVIE
     VAIVRPGPIQ GDMVHPYLRR RNKEEAITYP PKLKSVFERT LGVPLFQEQV MEVAIIAADY
     TPGEADQLRR AMAAWKRHGG LDPHRERLRT GMLANGYDAD FADRIFEQIK GFGSYGFPES
     HAASFALLTY ASCWLKCHEP AAFTCALINS WPMGFYSPDQ LLQDARRHHI EIRPVDVRYS
     HWDCTLEPLD HPDSTRNLAI RLGLRMLRSF REEDALRIEA ARSKRPFADA TDLAQRAGLD
     SRAAEALADS GALRGLIGHR HRARWEVAGV EAQRPLFDDV TSEEVQVTLP LPTVAEDLVA
     DYATLGTTLG PHPLALLRRQ LAAKRFRSSQ DLLSLENNRT LSVAGLVIGR QRPGTASGVT
     FVTLEDEFGM VNVVVWRDLA ERQRKVLVGS QLLQVFGRLE SNNGVRHLIA QRLYDLTPLL
     TGLEVRSRDF Q