ID   DNAE2_RALPJ             Reviewed;        1079 AA.
AC   B2UJ29;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=Rpic_4488;
OS   Ralstonia pickettii (strain 12J).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=402626;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=12J;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C.,
RA   Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Mikhailova N., Marsh T., Richardson P.;
RT   "Complete sequence of chromosome 2 of Ralstonia pickettii 12J.";
RL   Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; CP001069; ACD29580.1; -; Genomic_DNA.
DR   RefSeq; WP_012430460.1; NC_010678.1.
DR   AlphaFoldDB; B2UJ29; -.
DR   SMR; B2UJ29; -.
DR   STRING; 402626.Rpic_4488; -.
DR   EnsemblBacteria; ACD29580; ACD29580; Rpic_4488.
DR   KEGG; rpi:Rpic_4488; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_4; -.
DR   OMA; RGGKANC; -.
DR   OrthoDB; 561611at2; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1079
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_1000188732"
SQ   SEQUENCE   1079 AA;  120197 MW;  4E9E0BA3A6A67EA1 CRC64;
     MTSATPLPVL PDYAELHCIS NFTFLTGASH PSELVERAKL YGYQALALTD ECSVAGTARA
     LVASKEHELK LIIGSRFTVR NADGEPWLRL VLLAQNIEGY GNLSEIITHA RLAAPKGEYR
     LLADDLASPQ GELAHLRGAP DCLAILLPDY DPDPQQLLAQ AWWCQRVFGD RLSIALELPL
     RHADDRHRGT VLAVSEQIDV PMVATGGVQM HTRQRKPLHD VLTAIRLSKP LSECGLELAP
     SAEQAMRTRM QLAFLYQGEG GEKILRRTVE LAALCNFSLD EIEYEYPSEV VPEGMTPAEY
     LRAETFAGAA RRYPNGVSEK VHAQIEEELG LIAELGYEPF FLTVYDVVKF ARNEGILCQG
     RGSAANSAVC YCLGITEVNP DSGNTLFARF LSRARNEPPD IDVDFEHQRR EEVIQYIYKK
     YGVTRTALAA ALITYRTRSA LRDVGRALGI DLGIVEQVAK GQAWWDSRKE FAQRAGQQGL
     DPESPLVLRW AQLVDQLRGF PRHLSQHVGG FVIAQGKLSR LVPIENAAME NRRVIQWDKD
     DLESLRLLKV DVLALGMLTA IRRTLDMVDA LPGRREHYGA TAKLAMQNLP DDDTETYDMI
     CRAETIGVFQ IESRAQQSML PRLRPREYYD LVIQVAIVRP GPIQGGMVHP YLQRREAKRN
     GNTDCVTYPG PEVKAVLERT LGVPIFQEQV MEIAMKAGDF TADDADRLRR DMAAWKRKGN
     LTQHQERLVH AMVVKRRYDP AFVEALCKQM EGFAEYGFPE SHAAGFAKLA YVSSFLKCHE
     PAAFFAALLN SQPMGFYSPS QLVQEARRSH VRVLPADVTA SVWDSTLHPR ADGETGEDGL
     VQPDIRLGLN RIRGMRKPAA ERIEAARAQA PFTSVEDLAH RAALDRHDLN VLAAANALKS
     LAGHRRQALW QTLALQEPGH DHALLRQARP VEAPLELPAP PIGEEVMADY GSLGLSLQSH
     PVELLRARLE RMRFATAATL AGYRNGQLAR ACGIVTVRQR PGTANGTIFV SIEDETGAIN
     VILWPHLIER QRREVLNAQL LGVYGKWQCE RETRHLVAQH LVDLTPLLGR LATSSRDFH