ID   DNAE2_RALSO             Reviewed;        1075 AA.
AC   Q8XRN1;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=RSp0800;
GN   ORFNames=RS01907;
OS   Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OG   Plasmid megaplasmid Rsp.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=267608;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GMI1000;
RX   PubMed=11823852; DOI=10.1038/415497a;
RA   Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA   Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA   Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA   Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA   Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT   "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL   Nature 415:497-502(2002).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; AL646053; CAD17951.1; -; Genomic_DNA.
DR   RefSeq; WP_011004098.1; NC_003296.1.
DR   AlphaFoldDB; Q8XRN1; -.
DR   SMR; Q8XRN1; -.
DR   STRING; 267608.RSp0800; -.
DR   EnsemblBacteria; CAD17951; CAD17951; RSp0800.
DR   GeneID; 60503707; -.
DR   KEGG; rso:RSp0800; -.
DR   PATRIC; fig|267608.8.peg.4267; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_4; -.
DR   OMA; RGGKANC; -.
DR   Proteomes; UP000001436; Plasmid megaplasmid Rsp.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Plasmid;
KW   Reference proteome; Transferase.
FT   CHAIN           1..1075
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103393"
SQ   SEQUENCE   1075 AA;  119253 MW;  0602FCF53EDFDC60 CRC64;
     MNAPTPLPIG LPDYAELHCI SNFTFLTGAS HPHELVARAQ AFGYRALALT DECSVAGTVR
     AHMAAKEAGL KLIIGSRFTV RDAQGEPWLR LVLLAQDIDG YGNLCECITQ ARLAAPKGDY
     RLLADDLAAP AGELAHLRGM PHCLAILLPD YDPDPRHLLA QAQWCRQVFG DRLSMALELP
     LRHADDRHRG TVAAVSEQTD VPMVATGDVA MHSRQRKPLH DVLTAIRLSR PIAECGLALA
     PSAEQAMRTR MQLAFFYQGE RGAQVLRRSV ELASLCDFSL DEIAYEYPHE VVPEGQTPAG
     YLLAEVMAGA ARRYGKDIPS KVRAQLDEEL ALIAELRYEP FFLTVYDVVR FARSQSILCQ
     GRGSAANSAV CYCLGITEVN PESGNTLFAR FLSRARNEPP DIDVDFEHQR REEVIQYIYK
     KYGVTRTALA AALITYRTRS ALRDVGRALG IDLGVIEQVA KAQAWWDGRH EFAQRAGQQG
     LDPESPLVQR WAGLIEQLRG FPRHLSQHVG GFVIAQGKLS RLVPIENAAM PERRVIQWDK
     DDLESLRLLK VDVLALGMLT AIRRTLDMLD ALPGRRAHYG APDKLAMQHL PDEDTATYEM
     ICRAETIGVF QIESRAQQSM LPRLRPRTYY DLVIQVAIVR PGPIQGGMVH PYLQRREAVR
     NGNPDCVTYP GPEVKAVLER TLGVPIFQEQ VMEIAMKAGG FTADEADRLR RDMAAWKRKG
     NLTQHQARLM KEMVEVRHYD PAFVEALCRQ MEGFAEYGFP ESHAAGFARL AYVSSFLKCH
     EPAAFFAALL NSQPMGFYSP SQLVQEARRS RVRVLPADVT ASAWDSTLHA RPDGAGGQPD
     IRLGLNRIRG MRPAAGERIV VARAQAPFSS VEDLAHRAAL DRHDLSVLAA ANALLSLAGH
     RRQALWQTLA LQEPGQDHAL LRQARPVEAP LVLPAPSLGE EVVADYGSLG LSLQSHPLSL
     LRPRLERMRF VTAAALAGYR NGQLARACGI VTVRQRPGTA KGTIFVSIED ETGAVNVVLW
     PRLIERQRRE VLHARLLGVY GKWQCERETR HLVAQHLVDL TPLLGRLASS SRDFH