ID   DNAE2_RHILO             Reviewed;        1118 AA.
AC   Q98E34;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=mlr4428;
OS   Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)
OS   (Mesorhizobium loti (strain MAFF 303099)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Phyllobacteriaceae; Mesorhizobium.
OX   NCBI_TaxID=266835;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LMG 29417 / CECT 9101 / MAFF 303099;
RX   PubMed=11214968; DOI=10.1093/dnares/7.6.331;
RA   Kaneko T., Nakamura Y., Sato S., Asamizu E., Kato T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Ishikawa A., Kawashima K., Kimura T., Kishida Y.,
RA   Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Mochizuki Y.,
RA   Nakayama S., Nakazaki N., Shimpo S., Sugimoto M., Takeuchi C., Yamada M.,
RA   Tabata S.;
RT   "Complete genome structure of the nitrogen-fixing symbiotic bacterium
RT   Mesorhizobium loti.";
RL   DNA Res. 7:331-338(2000).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; BA000012; BAB51086.1; -; Genomic_DNA.
DR   RefSeq; WP_010912428.1; NC_002678.2.
DR   AlphaFoldDB; Q98E34; -.
DR   SMR; Q98E34; -.
DR   STRING; 266835.14024483; -.
DR   PRIDE; Q98E34; -.
DR   EnsemblBacteria; BAB51086; BAB51086; BAB51086.
DR   KEGG; mlo:mlr4428; -.
DR   PATRIC; fig|266835.9.peg.3493; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_5; -.
DR   OMA; VAKSHHW; -.
DR   OrthoDB; 561611at2; -.
DR   Proteomes; UP000000552; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1118
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103394"
FT   REGION          1071..1118
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1118 AA;  125208 MW;  3D66F8E487FDF6EA CRC64;
     MNALTVIPYA EFGIQSNFSF LRGASKPEEL VVAAKLMGFS AIGLADRNTV AGVVRAWQQA
     KVEKLAYHPG CRLVFGDGTP DILAYPRNRA GWGHLCRMLT QANLRDETEK GATLLQRDDL
     LEWGDLMSLA VLPDLAAGAE DSLALLGQIK DRFGRALRLG VSPCYAGYDR FRIEQAAALA
     QVAGIPLMAT NDVLYHTAER RPLQDVLTAI RLNTPVAEVG LELSANAERH LKPPLEMARL
     FRRHPQALAE TLRFAEELTF SLSDLQYNYP DEPTESGLGP QAELERLARE GAARRYPSGV
     PHSVIKRIEE ELALIERLNY ARYFLTVYDI VKYARSQDIL CQGRGSAANS IVCFCIGITE
     VGPDRIDTLF ERFISEERNE PPDIDVDFEH EKRETVIQYI YEKYSSKRTA LAAAVISYRG
     RSALREVSKA MGLSEDVRAS LSGSIWGWST SEVGEKEARA GGLDRSDPTS RHVMERANEI
     MGFPRHLSQH VGGFVITKDR LDEIVPIVKT AMDERKMVEW DKDDLDAVKI LKVDVLALGM
     LTCLQRAFIL LTDHYPKARD PYGQPYVLAS LPPEDKRVYD MIGRADTLGV FQIESRAQMS
     MLPRLKPKDF YDLVIEVAIV RPGPIQGDMV HPYLRRRQGK EKPEYQKPEL EAILSKTLGV
     PLFQEQAMKI AIVAGGFRPG EADELRRAMA TFKRTGTIGN YRQRMIDGMV GKGYTKDFAE
     RCFKQIEGFG EYGFPESHAA SFALLVYASC WFKTFYPDVF CAAILNSQPM GFYQPAQLVR
     DARDHGVDIR EVDVNCSAWD CTLEKVPFDP ARILPRHADM RGVIETNHAV RLGFRQIKGL
     SKERMEDFAK RRGSGYETVR DVWLRSGLDV DEIERLAQAD AFRSIGLDRR AALWEVRALG
     AKSAAEKLPL FDQPALRLRE LEPETKLPKM PLGEHVIHDY RSLGLSLKAH PVAFLRERLD
     RAGVTPNANL PSQRDGRRVS VAGLVLVRQR PGKGNAIFLT LEDDKAVANV IFWERTFTRF
     RPIVMGARFV KVSGKLQSES GVIHIVAEKI EDLTPWLTVL LEKVSGAGAP GPQPMGYAKE
     VGSDRRSRPE IGNAPARQDL ATLSEEAEQV MPKGRNFQ