ID   DNAE2_RUEPO             Reviewed;        1101 AA.
AC   Q5LVN0;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=SPO0670;
OS   Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter
OS   pomeroyi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Roseobacteraceae; Ruegeria.
OX   NCBI_TaxID=246200;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700808 / DSM 15171 / DSS-3;
RX   PubMed=15602564; DOI=10.1038/nature03170;
RA   Moran M.A., Buchan A., Gonzalez J.M., Heidelberg J.F., Whitman W.B.,
RA   Kiene R.P., Henriksen J.R., King G.M., Belas R., Fuqua C., Brinkac L.M.,
RA   Lewis M., Johri S., Weaver B., Pai G., Eisen J.A., Rahe E., Sheldon W.M.,
RA   Ye W., Miller T.R., Carlton J., Rasko D.A., Paulsen I.T., Ren Q.,
RA   Daugherty S.C., DeBoy R.T., Dodson R.J., Durkin A.S., Madupu R.,
RA   Nelson W.C., Sullivan S.A., Rosovitz M.J., Haft D.H., Selengut J., Ward N.;
RT   "Genome sequence of Silicibacter pomeroyi reveals adaptations to the marine
RT   environment.";
RL   Nature 432:910-913(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 700808 / DSM 15171 / DSS-3;
RX   PubMed=25780504; DOI=10.1186/1944-3277-9-11;
RA   Rivers A.R., Smith C.B., Moran M.A.;
RT   "An updated genome annotation for the model marine bacterium Ruegeria
RT   pomeroyi DSS-3.";
RL   Stand. Genomic Sci. 9:11-11(2014).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; CP000031; AAV93978.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q5LVN0; -.
DR   SMR; Q5LVN0; -.
DR   STRING; 246200.SPO0670; -.
DR   PRIDE; Q5LVN0; -.
DR   EnsemblBacteria; AAV93978; AAV93978; SPO0670.
DR   KEGG; sil:SPO0670; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_5; -.
DR   OMA; NSWPMGF; -.
DR   Proteomes; UP000001023; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1101
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103399"
FT   REGION          1055..1101
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1082..1101
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1101 AA;  122743 MW;  5D732922EEE73E1F CRC64;
     MTYAELCVTT NFTFLTGASH PEEMITRAAE LGLHAIAITD RNSLAGVVRA YAALKELRRE
     ADEAIRIRSQ HRIDSCSRQE VGHPQPIARP EAPRLPRLIT GCRLVLRDSP LHWIALPRDR
     AAYQRLTRLL TLGKRRAEKG DCHLDLADLL AGCTGMILIA LPQGRLDGAL PHLQQVQRRF
     PGHVFLGAAP RYDGSDQAWL AACARLALRS SAPMVAVGDA LMHRANRRQL ADVLTCMREH
     ITIDRIGTRA LPNAERRLKG HADMARLFRD HPAALRRTLD IAARCSFCLS ELSYEYPDEV
     AEGETPQARL ERLTAEGIVR RYPGGAPQRV HELVAKELKL VAELGFPAYF LTVHDIVQFA
     RSQGILCQGR GSAANSILCY LLGITDVSPD QIAMVFERFI SKYRGEPPDI DVDFEHERRE
     EVIQWIYQRY GRHRAGLCAT VIHFRSRAAI REVGKVMGLS QDVTASLSGQ IWGQSNGGAD
     PERMRELGLD PEDRRLALTI RLIGEIIGFP RHLSQHVGGF VITRGRLDEL APIENAAMED
     RTLIEWDKDD IDTLGILKVD VLGLGMLTCI RKAFDLMREH EQRHLSIDTV PQEDAATYDM
     LCAADAVGVF QVESRAQMNF LPRMRPRTFY DLVIEVAIVR PGPIQGGMVQ PYIRRRQGLE
     KAEPFGPELA AVTARTLGVP LFQEQALQIA VVGAGYTAEE ADHLRRSLAS FRRMGTIGAH
     RDTFIAGMRR NGYSQEVAER CFGQIEGFAD YGFPESHAAA FAMLAYVSAW LKCHHPAIFA
     CALLNAQPMG FYAPAQIVRD ARDHQVELRP ICVNASDWDN RLERRADGAL ALRLGFRQIK
     GFREEDAAWI VAARGNGYPD PQALWLRAGV TPAVLERLAE ADAFADMGIG RRDALWQVRA
     IRGQAPLPLF NDPLDGEIIH EPQVTLPAMH LGEEVVEDYV SMRLTLRAHP MELLRPAIPG
     LTPHDKLASV AAHRVSVCGL VITRQRPGTA SGVIFLTLED ETGVSNVVVW PKIYEQFRRI
     VMGGRLLRVT GTLQREGIVV HLIAQRIEDL SPRLADLGHP MDSAVGQTTP QTDSAPRPRP
     QPRAMHPREQ AKRLFPSRDF H