ID   DNAE2_SINFN             Reviewed;        1117 AA.
AC   C3M9Z5;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=NGR_c31860;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; CP001389; ACP26920.1; -; Genomic_DNA.
DR   RefSeq; WP_012709668.1; NC_012587.1.
DR   RefSeq; YP_002827673.1; NC_012587.1.
DR   AlphaFoldDB; C3M9Z5; -.
DR   SMR; C3M9Z5; -.
DR   STRING; 394.NGR_c31860; -.
DR   PRIDE; C3M9Z5; -.
DR   EnsemblBacteria; ACP26920; ACP26920; NGR_c31860.
DR   KEGG; rhi:NGR_c31860; -.
DR   PATRIC; fig|394.7.peg.6024; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_5; -.
DR   OMA; VAKSHHW; -.
DR   OrthoDB; 561611at2; -.
DR   Proteomes; UP000001054; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1117
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_1000188733"
FT   REGION          1086..1117
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1117 AA;  125432 MW;  EADA2DE0E895C4F4 CRC64;
     MSGEPTFHEL GARTNFSFLE GAAPAEEMIV FAKKAGLSGL GVADRNSVAG VVRAHAKAKV
     EDYPFQPGAR LVFADATPDI LAYPKNRRGW GHLCRLLSVG NLRAKKGDCT LYLADLLEWQ
     EDLLLIVMPG EGRPQPEILE ALLEKLKASA GNRLYLGLAP RYDSFDRHDF AVLATVARSL
     DVRLLATNDA LYHDPHYRPL ADVVTAIREH VPIASAGFLL QKNAERHLKG PKEMVRLFRD
     YPEAIVNANK FFKRLSFSLD ELSHQYPDEN AEGETPAESL RRLVAEGALE RYPSGVPEKV
     TRQIEYELEL IHDKKYEPYF LTVHKLVKFA RSVNILCQGR GSAANSSVCF CLGITDVDPQ
     KFTLLFDRFL SRDRDEPPDI DVDFEHERRE EVIQYIYRTY GKEHAGLTAA VISYRSRSAG
     REVAKAFGLS EDVQSALVSS VWGWGTSPFT EEQAKGAGLD MADPLTRRVL AYASLLMNFP
     RHLSQHVGGF VITRDRLDEV VPIMNTAMPD RYMIEWDKDD LDQLKILKVD VLALGMLTCL
     ARAFRFLEVH YGKPKTLAEL YEDQQAAVYD MICRADTVGV FQIESRAQMS MLPRLRPREM
     YDLVIEVAIV RPGPIQGNMV HPYLKRREAQ RKGEPVDYPS PELKAVLERT LGVPLFQEQA
     MQIAITAAGF SPSEADRLRR AMATFKRTGT IHTFERKMID GMVRNGYERE FAERCFNQIK
     GFGEYGFPES HAASFASLVY ASSWFKAYYP DIFCAALLNS QPMGFYAPAQ LVRDAREHGV
     RILPVDVNHS DWDALLEGEA MFRKEAIDPR HGEMRDVIRS GKAVRLGFRL VKGLRQPDME
     ALVARRGEGY RSVHDLWLRS GLTRAVLERL ADADAFRSIG LDRRRALWAV KALNEQSAVE
     RLPLFDGAGS TDLQVEPAVA LPDMAAGEHV IHDYRYLTLS LKAHPVSFMR GDFARMGILS
     SRDLARTPAG RRVTVAGLVL VRQRPGSANG VIFMTIEDET GVANIIVWEK TFQKYRPQVM
     GSRLVKVRGR LQSESGVIHV VAEYMEDMTP MLGLLRNEAR RFGANDRADE ALRPTADARD
     KKALRQLRPA PPAQEGLETS EAGSAVAEVM PKGRNFH