ID   DNAE2_SINMW             Reviewed;        1116 AA.
AC   A6UDW8;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=Smed_3021;
OS   Sinorhizobium medicae (strain WSM419) (Ensifer medicae).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=366394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=WSM419;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G.,
RA   Richardson P.;
RT   "Complete sequence of Sinorhizobium medicae WSM419 chromosome.";
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; CP000738; ABR61848.1; -; Genomic_DNA.
DR   RefSeq; WP_012067229.1; NC_009636.1.
DR   RefSeq; YP_001328683.1; NC_009636.1.
DR   AlphaFoldDB; A6UDW8; -.
DR   SMR; A6UDW8; -.
DR   STRING; 366394.Smed_3021; -.
DR   PRIDE; A6UDW8; -.
DR   EnsemblBacteria; ABR61848; ABR61848; Smed_3021.
DR   KEGG; smd:Smed_3021; -.
DR   PATRIC; fig|366394.8.peg.6246; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_4_0_5; -.
DR   OMA; VAKSHHW; -.
DR   OrthoDB; 561611at2; -.
DR   Proteomes; UP000001108; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   PANTHER; PTHR32294:SF4; PTHR32294:SF4; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Transferase.
FT   CHAIN           1..1116
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_1000070598"
SQ   SEQUENCE   1116 AA;  124942 MW;  EFEAF91A4053FDD2 CRC64;
     MSGDTAFYEL GACTNFSFLE GAAPAEEMVV FAKKARLAGL GIADRNSVAG VVRAHAKAKM
     ENYPFQPGAR LVFADGTPDV LAYPRNRRGW GHLCRLLSAG NLRSKKGDCT LHLADLLEWQ
     EELLLIVMPD RVRPEPESLK PLLGKLQEHA GNRLYLGLAP RYDGFDRHDF AVLATVARKA
     GIGLLATNDA LYHDPDYRPL ADVVTAIREH VPVAGAGFLL QKNAERHLKS PREMARLFSD
     YPEAIANTQK FFRHLAFSLD ELRHQYPDEN AGGETPAESL RRLVSEGAAE RYPEGVPEKV
     QRQIEYELEL INDKKYEPYF LTVHKLVKFA RSEKILCQGR GSAANSSVCF CLGITDVDPQ
     KFTLLFDRFL SKDRDEPPDI DVDFEHERRE EVIQYIYRTY GKEHAGLAAA VISYRSRSAG
     REVAKAFGFS EDVQSALVSS IWGWGNSPFT EEQARGAGLD AADPSTRRVL AYASLLMNYP
     RHLSQHVGGF VITRDRLDEV VPIMNTAMPD RYMIEWDKDD LDELKILKVD VLALGMLTCL
     AKGFKLLEAH YGEPITLAEI YQDHQPAVYD MICRADTVGV FQIESRAQMS MLPRLQPREM
     YDLVIEVAIV RPGPIQGNMV HPYLKRREGQ RKGEKVKYPS PELKAVLERT LGVPLFQEQA
     MQIAITAAGF SPSEADRLRR AMATFKRTGT IHTFERKMVE GMVANGYERE FAERCFNQIK
     GFGEYGFPES HAASFASLVY ASAWLKTYYP DIFCAALLNA QPMGFYAPAQ LVRDAREHGV
     KVLPVDINHS DWDALLEGEG QFRKESVDPR HADMREVIKT RKAVRLGFRL VKGLKQADMG
     ALVACRGEGY RSVHDLWFRS GLSRSVLERL ADADAFRSLG LDRRAALWAV KALDEQSAVE
     RLPLFEGAGS LDLRAEPKVA LPEMPAGEQV IHDYRTLTLS LKAHPVSFMR EDFSRTGILR
     SRDLAATATG KWVTVAGLVL VRQRPGSANG VIFMTIEDET GIANIIVWEK TFRKYRPQVM
     GSRLVKIRGR LQNQSGVIHV VADHLEDITP MLGLLRREAR RFGANDRADG ALRPSGDARE
     KRKLRQLRLG LPGGAEPEGE AAAQVAEAMP KGRNFH