ID   DNAE2_SYMTH             Reviewed;        1085 AA.
AC   Q67N73;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Error-prone DNA polymerase {ECO:0000255|HAMAP-Rule:MF_01902};
DE            EC=2.7.7.7 {ECO:0000255|HAMAP-Rule:MF_01902};
GN   Name=dnaE2 {ECO:0000255|HAMAP-Rule:MF_01902}; OrderedLocusNames=STH1885;
OS   Symbiobacterium thermophilum (strain T / IAM 14863).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Symbiobacteriaceae;
OC   Symbiobacterium.
OX   NCBI_TaxID=292459;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=T / IAM 14863;
RX   PubMed=15383646; DOI=10.1093/nar/gkh830;
RA   Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K.,
RA   Ikeda H., Hattori M., Beppu T.;
RT   "Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium
RT   that depends on microbial commensalism.";
RL   Nucleic Acids Res. 32:4937-4944(2004).
CC   -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC       translesion synthesis (TLS). It is not the major replicative DNA
CC       polymerase. {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.7; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01902};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01902}.
CC   -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01902}.
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DR   EMBL; AP006840; BAD40870.1; -; Genomic_DNA.
DR   RefSeq; WP_011196012.1; NC_006177.1.
DR   AlphaFoldDB; Q67N73; -.
DR   SMR; Q67N73; -.
DR   STRING; 292459.STH1885; -.
DR   EnsemblBacteria; BAD40870; BAD40870; STH1885.
DR   KEGG; sth:STH1885; -.
DR   eggNOG; COG0587; Bacteria.
DR   HOGENOM; CLU_001600_0_0_9; -.
DR   OMA; NSWPMGF; -.
DR   OrthoDB; 561611at2; -.
DR   Proteomes; UP000000417; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.1600; -; 1.
DR   HAMAP; MF_01902; DNApol_error_prone; 1.
DR   InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR   InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR   InterPro; IPR040982; DNA_pol3_finger.
DR   InterPro; IPR023073; DNA_pol_error_prone.
DR   InterPro; IPR029460; DNAPol_HHH.
DR   InterPro; IPR004013; PHP_dom.
DR   InterPro; IPR003141; Pol/His_phosphatase_N.
DR   InterPro; IPR016195; Pol/histidinol_Pase-like.
DR   InterPro; IPR004805; PolC_alpha.
DR   PANTHER; PTHR32294; PTHR32294; 1.
DR   Pfam; PF07733; DNA_pol3_alpha; 1.
DR   Pfam; PF17657; DNA_pol3_finger; 1.
DR   Pfam; PF14579; HHH_6; 1.
DR   Pfam; PF02811; PHP; 1.
DR   SMART; SM00481; POLIIIAc; 1.
DR   SUPFAM; SSF89550; SSF89550; 1.
DR   TIGRFAMs; TIGR00594; polc; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; DNA replication;
KW   DNA-directed DNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..1085
FT                   /note="Error-prone DNA polymerase"
FT                   /id="PRO_0000103400"
SQ   SEQUENCE   1085 AA;  120631 MW;  1BC11470BF0DB77E CRC64;
     MSFVHLHVHS PFSFLDGASR LDDLIAEAAL HGMPALALTD HNNVSGAVRF QRKAQEAGIQ
     PIQGAELTLE GGYHLTLLAT GPKGYANLNR LITAAHLGQE PRATPGAMFA LAPGHDRLQP
     ACPLSALSHD TDGLIALSGC RRGEIPSLIL RGRLAEAEAA ARRYAAWFPG RFYLELHDER
     LPGEQALNRA LRDLGEALGL PLVVTSNVHH RTREEFFVHD LLTCVRTLTR IDQPHPERPL
     NDQRYLKPPE VMIERFREFP EAVKNTLAIA ERCRPVLDLD ARLHPEFQTP DGSHPAAYLT
     HLVYDGAARR YGRITERIRR RLEHELTIIT RLGYEDYFLL VWDVVRFARE RGIRCAGRGS
     AADSAVAYCL FITDVDAIER GLLFERFMSL ERAQKPDIDI DFDARRRDEV AAYVYRKYGA
     DHVASVCTYN TFQARSAVRD LGKAMGFQEA DLDYLAKRIP WHAGADEILT VMARYPELRQ
     SGIPWHKFEQ LVQAAERVAR FPRFIGTHLG GLVISRRPLL EVTPLQMAAK GQVVCQFDKE
     YVEDLGLVKL DLLSLRTFTA VDDAVRAIGD IDYEQIPHGD AATYEMLGVG ESIGVFQLES
     PAQRALQARL KPDRFEDIVA SVAIIRPGPI KGNMVEPFLA RRHGKEEVTY LHPKLKPILE
     KTYGVVLFQE QVIEIATAVA GFTPGEADQL RRVMTHARNR EDMEAIGRLF RQKAAAAGVD
     PAVADTIFSY IQGYASYGFC EAHAAAFANT AYKTAYLVRH YPAEYFAALM SAQPMGYYPI
     NTLAVEARRR GVGLLPVDIN RSEEAFTVEE WSREAWEAFW GMEPFAPEYP ATGKAIRIGL
     RAVKGVGEPA AAAILAARDG GEFRSLADLV RRTGGAVPRG PLEALVLAGA FDRLHSNRRA
     ALWQVAHLLE AERIRQERGA GQAGLLDGDA PGLGAQAAEA LEAASPQGTP AIADFGAAER
     YLKEYELTGL MVRTHFMRFV RERLAREGYL TAQEVRQRRA GELVKVAGLP VCPHRPPTRS
     GKIVVFLSLE DETGLIDLTI FEDVYQRYGH LIFTDPRPPL AALGRVDRRG GHVSITVNRL
     RLLDT