DNAI1_MOUSE
ID DNAI1_MOUSE Reviewed; 701 AA.
AC Q8C0M8; Q8BKY5;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Dynein axonemal intermediate chain 1;
DE AltName: Full=Axonemal dynein intermediate chain 1;
GN Name=Dnai1; Synonyms=Dnaic1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Corpus striatum, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Part of the dynein complex of respiratory cilia.
CC {ECO:0000250}.
CC -!- SUBUNIT: Consists of at least two heavy chains and a number of
CC intermediate and light chains. Interacts with BICD2 (By similarity).
CC Interacts with CFAP45 and CFAP52 (By similarity).
CC {ECO:0000250|UniProtKB:Q9UI46}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000250|UniProtKB:Q9UI46}.
CC -!- SIMILARITY: Belongs to the dynein intermediate chain family.
CC {ECO:0000305}.
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DR EMBL; AK030213; BAC26847.1; -; mRNA.
DR EMBL; AK047808; BAC33160.1; -; mRNA.
DR CCDS; CCDS18066.1; -.
DR RefSeq; NP_780347.2; NM_175138.4.
DR AlphaFoldDB; Q8C0M8; -.
DR SMR; Q8C0M8; -.
DR BioGRID; 213117; 3.
DR STRING; 10090.ENSMUSP00000100028; -.
DR iPTMnet; Q8C0M8; -.
DR PhosphoSitePlus; Q8C0M8; -.
DR MaxQB; Q8C0M8; -.
DR PaxDb; Q8C0M8; -.
DR PRIDE; Q8C0M8; -.
DR ProteomicsDB; 277347; -.
DR Antibodypedia; 11255; 228 antibodies from 34 providers.
DR DNASU; 68922; -.
DR Ensembl; ENSMUST00000102963; ENSMUSP00000100028; ENSMUSG00000061322.
DR GeneID; 68922; -.
DR KEGG; mmu:68922; -.
DR UCSC; uc008sjc.2; mouse.
DR CTD; 27019; -.
DR MGI; MGI:1916172; Dnai1.
DR VEuPathDB; HostDB:ENSMUSG00000061322; -.
DR eggNOG; KOG1587; Eukaryota.
DR GeneTree; ENSGT00940000156436; -.
DR HOGENOM; CLU_015820_2_0_1; -.
DR InParanoid; Q8C0M8; -.
DR OMA; FNYCERA; -.
DR OrthoDB; 704698at2759; -.
DR PhylomeDB; Q8C0M8; -.
DR TreeFam; TF300553; -.
DR BioGRID-ORCS; 68922; 3 hits in 71 CRISPR screens.
DR PRO; PR:Q8C0M8; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8C0M8; protein.
DR Bgee; ENSMUSG00000061322; Expressed in lumbar subsegment of spinal cord and 83 other tissues.
DR ExpressionAtlas; Q8C0M8; baseline and differential.
DR Genevisible; Q8C0M8; MM.
DR GO; GO:0097729; C:9+2 motile cilium; IDA:MGI.
DR GO; GO:0005813; C:centrosome; IDA:CAFA.
DR GO; GO:0005929; C:cilium; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IDA:CAFA.
DR GO; GO:0005576; C:extracellular region; IEA:GOC.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0036157; C:outer dynein arm; ISO:MGI.
DR GO; GO:0045504; F:dynein heavy chain binding; IBA:GO_Central.
DR GO; GO:0045503; F:dynein light chain binding; IBA:GO_Central.
DR GO; GO:0003341; P:cilium movement; ISO:MGI.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:MGI.
DR GO; GO:0003351; P:epithelial cilium movement involved in extracellular fluid movement; IMP:MGI.
DR GO; GO:0030317; P:flagellated sperm motility; ISO:MGI.
DR GO; GO:0007507; P:heart development; IMP:MGI.
DR GO; GO:0008286; P:insulin receptor signaling pathway; IDA:CAFA.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR GO; GO:0036158; P:outer dynein arm assembly; ISO:MGI.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cytoplasm; Cytoskeleton; Dynein; Microtubule;
KW Motor protein; Phosphoprotein; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..701
FT /note="Dynein axonemal intermediate chain 1"
FT /id="PRO_0000254655"
FT REPEAT 382..422
FT /note="WD 1"
FT REPEAT 431..474
FT /note="WD 2"
FT REPEAT 539..579
FT /note="WD 3"
FT REPEAT 581..621
FT /note="WD 4"
FT REPEAT 629..668
FT /note="WD 5"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 119..165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 136..155
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 124
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UI46"
FT MOD_RES 127
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UI46"
FT CONFLICT 636
FT /note="H -> R (in Ref. 1; BAC33160)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 701 AA; 79758 MW; 4FC542B80B37931E CRC64;
MPSKQIRKQS ISVTRGARRR DEDSGTDVGE GTDEWSQSKA TVRPPDQLEL TDAELKEEFT
RILTANNPHA PQNIVRYSFK EGTYKLIGFV NQMAVHFSQV GNLIPKDSDE GRRQHYRDEM
VAGSQESIKV VTSEAENLEE EEEPKEGEGE AEAEAEAGSQ TDIPAAAETT EKVIEEELMA
PVQPKERKLT NQFNFSERAS QTFNNPLRDR ECQMEPPPRT NFSATANQWE IYDAYVDELE
KQEKTKEKEK AKTPVAKKTE KMAMRKLTSM ESQSDDITKV TQAAKIVERM VNQNTYDDVA
QDFKYYEDTA DEYRDQEGTL LPLWKFQNDK AKRLAVTALC WNPKYKDLFA VGHGSYDFMK
QSRGMLLLYS MKNPSFPEYM FSSESGIMCL DVHVDHPYLV VVGYYDGNVA IYNLKKPHSQ
PCFRSTSKSG KHTDPVWQVK WQKDDMDHNL NFFSVSSDGR IVSWTLVKSE LVHIDIIKLK
TEGSTTEIPE GLQLHTVGCG TAFDFHKEID YMFLVGTEEG KIYKCSKSYS SQFLDTYDAH
NMAVDAVLWN PYHTKVFMSC SSDWTVKIWD HTIKTPMFIY DLNSAVGDVA WAPYSSTVFA
AVTTDGKAHV FDLAVNKYEA ICNQPVVAKK KNKITHVQFN PIHPIIIVGD DRGHIICLKL
SPNLRKMPKE KKGQEVQKGP AVEIAKLDKL LNLVREVKTK T
