DNAI2_MOUSE
ID DNAI2_MOUSE Reviewed; 623 AA.
AC A2AC93; A2AC94;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Dynein axonemal intermediate chain 2;
DE AltName: Full=Axonemal dynein intermediate chain 2;
GN Name=Dnai2; Synonyms=Dnaic2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=C57BL/6J; TISSUE=Ovary;
RX PubMed=18547164; DOI=10.1089/dna.2008.0752;
RA Yang Z., Wu J.;
RT "Mouse dynein axonemal intermediate chain 2: cloning and expression.";
RL DNA Cell Biol. 27:479-488(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP INTERACTION WITH DNAAF2.
RX PubMed=19052621; DOI=10.1038/nature07471;
RA Omran H., Kobayashi D., Olbrich H., Tsukahara T., Loges N.T., Hagiwara H.,
RA Zhang Q., Leblond G., O'Toole E., Hara C., Mizuno H., Kawano H.,
RA Fliegauf M., Yagi T., Koshida S., Miyawaki A., Zentgraf H., Seithe H.,
RA Reinhardt R., Watanabe Y., Kamiya R., Mitchell D.R., Takeda H.;
RT "Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins.";
RL Nature 456:611-616(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP INTERACTION WITH DNAAF6.
RX PubMed=24421334; DOI=10.1083/jcb.201304076;
RA Dong F., Shinohara K., Botilde Y., Nabeshima R., Asai Y., Fukumoto A.,
RA Hasegawa T., Matsuo M., Takeda H., Shiratori H., Nakamura T., Hamada H.;
RT "Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse
RT sperm.";
RL J. Cell Biol. 204:203-213(2014).
CC -!- FUNCTION: Part of the dynein complex of respiratory cilia.
CC {ECO:0000250}.
CC -!- SUBUNIT: Consists of at least two heavy chains and a number of
CC intermediate and light chains (Probable). Interacts with DNAAF2
CC (PubMed:19052621). Interacts with DNAAF6/PIH1D3 (PubMed:24421334).
CC Interacts with HEATR2; probably involved in outer arm dynein assembly
CC (By similarity). {ECO:0000250|UniProtKB:Q9GZS0,
CC ECO:0000269|PubMed:19052621, ECO:0000269|PubMed:24421334, ECO:0000305}.
CC -!- INTERACTION:
CC A2AC93; Q8BPI1: Dnaaf2; NbExp=2; IntAct=EBI-15744757, EBI-15744709;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000250|UniProtKB:Q9GZS0}. Dynein axonemal particle
CC {ECO:0000250|UniProtKB:Q4QR00}. Note=Located in the proximal region of
CC respiratory cilia. {ECO:0000250|UniProtKB:Q9GZS0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A2AC93-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A2AC93-2; Sequence=VSP_036542;
CC -!- TISSUE SPECIFICITY: Predominantly expressed in ovary, testis and lung.
CC {ECO:0000269|PubMed:18547164}.
CC -!- DEVELOPMENTAL STAGE: In ovaries, it is detected at high levels in vivo
CC on day 10, with a subsequent decrease on days 15 and 20, in adult and
CC old ovaries. Weakly expressed on day 5. {ECO:0000269|PubMed:18547164}.
CC -!- SIMILARITY: Belongs to the dynein intermediate chain family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAM19252.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; EF565270; ABU62629.1; -; mRNA.
DR EMBL; AL663079; CAM19252.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL663079; CAM19253.1; -; Genomic_DNA.
DR EMBL; CH466558; EDL34441.1; -; Genomic_DNA.
DR CCDS; CCDS36366.1; -. [A2AC93-1]
DR RefSeq; NP_001030050.2; NM_001034878.2. [A2AC93-1]
DR RefSeq; XP_006533723.1; XM_006533660.2. [A2AC93-1]
DR RefSeq; XP_006533724.1; XM_006533661.1. [A2AC93-1]
DR RefSeq; XP_006533725.1; XM_006533662.3. [A2AC93-1]
DR AlphaFoldDB; A2AC93; -.
DR SMR; A2AC93; -.
DR DIP; DIP-59778N; -.
DR IntAct; A2AC93; 1.
DR STRING; 10090.ENSMUSP00000065787; -.
DR iPTMnet; A2AC93; -.
DR PhosphoSitePlus; A2AC93; -.
DR MaxQB; A2AC93; -.
DR PaxDb; A2AC93; -.
DR PRIDE; A2AC93; -.
DR ProteomicsDB; 279453; -. [A2AC93-1]
DR Antibodypedia; 31949; 220 antibodies from 29 providers.
DR DNASU; 432611; -.
DR Ensembl; ENSMUST00000069325; ENSMUSP00000065787; ENSMUSG00000034706. [A2AC93-1]
DR Ensembl; ENSMUST00000092469; ENSMUSP00000090126; ENSMUSG00000034706. [A2AC93-1]
DR GeneID; 432611; -.
DR KEGG; mmu:432611; -.
DR UCSC; uc007mfn.1; mouse. [A2AC93-1]
DR CTD; 64446; -.
DR MGI; MGI:2685574; Dnai2.
DR VEuPathDB; HostDB:ENSMUSG00000034706; -.
DR eggNOG; KOG1587; Eukaryota.
DR GeneTree; ENSGT00940000161939; -.
DR InParanoid; A2AC93; -.
DR OMA; WDFFYRQ; -.
DR PhylomeDB; A2AC93; -.
DR TreeFam; TF300553; -.
DR BioGRID-ORCS; 432611; 3 hits in 70 CRISPR screens.
DR PRO; PR:A2AC93; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; A2AC93; protein.
DR Bgee; ENSMUSG00000034706; Expressed in spermatid and 83 other tissues.
DR ExpressionAtlas; A2AC93; baseline and differential.
DR Genevisible; A2AC93; MM.
DR GO; GO:0097729; C:9+2 motile cilium; IDA:MGI.
DR GO; GO:0005858; C:axonemal dynein complex; ISO:MGI.
DR GO; GO:0005930; C:axoneme; IDA:MGI.
DR GO; GO:0005929; C:cilium; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0120293; C:dynein axonemal particle; ISS:UniProtKB.
DR GO; GO:0009897; C:external side of plasma membrane; IDA:MGI.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0036157; C:outer dynein arm; ISO:MGI.
DR GO; GO:0036126; C:sperm flagellum; ISO:MGI.
DR GO; GO:0045504; F:dynein heavy chain binding; IBA:GO_Central.
DR GO; GO:0045503; F:dynein light chain binding; IBA:GO_Central.
DR GO; GO:0003777; F:microtubule motor activity; ISO:MGI.
DR GO; GO:0060271; P:cilium assembly; ISO:MGI.
DR GO; GO:0003341; P:cilium movement; ISO:MGI.
DR GO; GO:0007368; P:determination of left/right symmetry; ISO:MGI.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR GO; GO:0036158; P:outer dynein arm assembly; ISO:MGI.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium;
KW Cilium biogenesis/degradation; Cytoplasm; Cytoskeleton; Dynein;
KW Microtubule; Motor protein; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..623
FT /note="Dynein axonemal intermediate chain 2"
FT /id="PRO_0000365816"
FT REPEAT 214..254
FT /note="WD 1"
FT REPEAT 261..302
FT /note="WD 2"
FT REPEAT 362..401
FT /note="WD 3"
FT REPEAT 405..445
FT /note="WD 4"
FT REPEAT 450..489
FT /note="WD 5"
FT REGION 565..602
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 450..461
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036542"
SQ SEQUENCE 623 AA; 70968 MW; A31B5EF749EF7FF4 CRC64;
MEIVYVYLKK RSEFGKQCNF SDRQAELNID ILPNPELAAL YVERNPVDTG IQCSASMSEH
EANTERFEME SCGVNHVEGG WPKDVNPQEL EQTIRFRKKV EKDENYINAV MQLGSIMEHC
IKQNNAIDIY EEYFDDEEAV EVTEEAPSAK TINVFRDPQE IKRTATHLSW HPDGNRKLAV
AYSCLKFQRA PMSMNYDSYI WDLENPNRPE IALKPLSPLV TLEYNPKDSH VLLGGCYNGQ
IACWDTRKGS LVAELSTIEF SHRDPVYGTI WLQSKTGTEC FSASTDGQVM WWDIRKISEP
IEVVIMDISR KEQLENALGA ISLEFESTLP TKFMVGTEQG IVISCNRKAK TQAEKIVCTF
YGHHGPIYAL QRNPFYPKNF LTVGDWTARI WSEDSRESSI MWTKYHMAYL SDGAWSPVRP
AVFFTTKMDG TLDIWDLVFK QCDPALSLKV CDDPLFCLRV QDNGCLIACG SELGTTTLLE
VSSSLSTLQR NEKNIASSIF ERETRREKIL EARHREMRLK EKGKVEGKED DQKEEEAALD
LDELVGKAEE EFFEVIFSEL KRKEAEALKK KPKPRKKSSV KVEAEEEVEE NVGEEEEAGG
IIGIDAVEDM SEEAGEEQED VPT
