DNAI3_DANRE
ID DNAI3_DANRE Reviewed; 968 AA.
AC A0A0G2KIZ8;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 07-OCT-2020, sequence version 3.
DT 03-AUG-2022, entry version 40.
DE RecName: Full=Dynein axonemal intermediate chain 3;
DE AltName: Full=WD repeat domain 63;
GN Name=dnai3; Synonyms=wdr63;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP DISRUPTION PHENOTYPE, FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=29285825; DOI=10.1002/humu.23388;
RA Hofmeister W., Pettersson M., Kurtoglu D., Armenio M., Eisfeldt J.,
RA Papadogiannakis N., Gustavsson P., Lindstrand A.;
RT "Targeted copy number screening highlights an intragenic deletion of WDR63
RT as the likely cause of human occipital encephalocele and abnormal CNS
RT development in zebrafish.";
RL Hum. Mutat. 39:495-505(2018).
CC -!- FUNCTION: May be involved in the regulation of cilia function.
CC {ECO:0000269|PubMed:29285825}.
CC -!- SUBUNIT: Part of the multisubunit axonemal dynein complex formed at
CC least of two heavy chains and a number of intermediate and light
CC chains. {ECO:0000250|UniProtKB:B2RY71}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8IWG1}.
CC -!- DEVELOPMENTAL STAGE: Expressed maternally and throughout early
CC developmental stages. {ECO:0000269|PubMed:29285825}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown of the protein results in a
CC ciliopathy like body curvature. Morphant embryos show a reduction and
CC dis-organization of floor plate motile cilia and a significant
CC reduction in primary cilia of the dorsal neural tube. No severe brain
CC abnormalities is observed. {ECO:0000269|PubMed:29285825}.
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DR EMBL; CABZ01069335; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CABZ01069336; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CABZ01069337; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CABZ01069338; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; LO017850; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; A0A0G2KIZ8; -.
DR ZFIN; ZDB-GENE-121116-5; dnai3.
DR eggNOG; KOG1587; Eukaryota.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0036156; C:inner dynein arm; IBA:GO_Central.
DR GO; GO:0045504; F:dynein heavy chain binding; IBA:GO_Central.
DR GO; GO:0045503; F:dynein light chain binding; IBA:GO_Central.
DR GO; GO:0003341; P:cilium movement; IMP:UniProtKB.
DR GO; GO:0060294; P:cilium movement involved in cell motility; IBA:GO_Central.
DR GO; GO:0036159; P:inner dynein arm assembly; IBA:GO_Central.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 2.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
PE 2: Evidence at transcript level;
KW Coiled coil; Cytoplasm; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..968
FT /note="Dynein axonemal intermediate chain 3"
FT /id="PRO_0000450865"
FT REPEAT 407..447
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 480..536
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 712..753
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REGION 1..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 136..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 897..930
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 830..857
FT /evidence="ECO:0000255"
FT COMPBIAS 1..28
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 143..157
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 968 AA; 110941 MW; DFE6F2D06701F806 CRC64;
MKDTSSKRPK SKEANKKKTK DKSNADNLPK PEEAAASEPG HPEYIFPLVL TSVSQELFGC
RADEDVTAEN PHKLLKKQDI IQDLRHRAAV CDFSPVKQAV LDYPEDELLL VFDRDFIYGQ
SFYLVLTVQA KESILKPPAE GADEQMEDEE QQEEEEEMMT KTPEPQPWVS LGSERLVYTP
SAVLSSVICV RQLRYSVCPL GRRSGTRVRF SDRNALDWKE ELVECSSYED KSFSITRMER
DSSTQTCRDN THTCTQTLWK KPKNMWSQYE PREFSSEEKQ HQLQSENLKN FITSVARRFE
IYLQQNLIAD VFCDDWTALC EDDASLMGKT ETQLKEYQSF MDLHFSKEKT ISHVQWHPSI
SGVIVVSMME RLSLEERIDS STKLLLNPSH ILFWSFSDPI NPQLQLECPD DVLCFQFSPS
DHNIIAGGCM NGQVVLWDIS AHVDRLQDTR SGGKNILNKL GKSDATPVVR YCAVSGIENG
HKAPITDIQW LPETFEVNKL GTPVENQSRT SVQLVTCAPD CCVLFWDLRA PRVMVHSLTD
IKQKQEEKPL ENPHGVPNTF THLNLTWKPF IRVSLPKISS SGEYSPLRFS MRENTVDYNT
GDKSVSGADS RVFAQLRMQS AKQQKPLQNI STKLYIATED GELVYTDWKV EKDNDSGRML
SAKPLDVFLL QDTLLHTVSR SPFFRDIILT VGRFSFSIWR EGLTCGPLLM SVYSKSMFSA
GHWSRSRPAV LFMGREDGDV EVWDLLQNSR QPSHTQNIST AAISCIRTQT SSGNTHPYTE
TLHTHTHTQP PSAAYAHRPA PVTHTHTHKH CTHTHTHTHT HTHTHSRHQL HTHTDQLRVL
EERVREAKQN LLAVSDRLGT LHILQIPGSL RRSSSSERQN VEKYFEKEEE RLQYFEKRQS
DHQKKKKETE AEQQKKKTEL VTPPKQEEEV NAETLKEYQQ FLTLEKLILK DMNIQNETQQ
TCNTINTP
