DNAI3_MOUSE
ID DNAI3_MOUSE Reviewed; 923 AA.
AC B2RY71;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Dynein axonemal intermediate chain 3 {ECO:0000305};
DE AltName: Full=WD repeat-containing protein 63;
GN Name=Dnai3; Synonyms=Wdr63 {ECO:0000312|MGI:MGI:3045269};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION.
RX PubMed=25498833; DOI=10.1016/j.joen.2014.09.027;
RA Diao S., Yang D.M., Dong R., Wang L.P., Wang J.S., Du J., Wang S.L.,
RA Fan Z.;
RT "Enriched trimethylation of lysine 4 of histone H3 of WDR63 enhanced
RT osteogenic differentiation potentials of stem cells from apical papilla.";
RL J. Endod. 41:205-211(2015).
RN [4]
RP DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=26501274; DOI=10.3390/ijms161024732;
RA Young S.A., Miyata H., Satouh Y., Kato H., Nozawa K., Isotani A.,
RA Aitken R.J., Baker M.A., Ikawa M.;
RT "CRISPR/Cas9-mediated rapid generation of multiple mouse lines identified
RT Ccdc63 as essential for spermiogenesis.";
RL Int. J. Mol. Sci. 16:24732-24750(2015).
RN [5]
RP SUBUNIT, AND FUNCTION.
RX PubMed=30060180; DOI=10.1093/jmcb/mjy043;
RA Zhang Y., Chen Y., Zheng J., Wang J., Duan S., Zhang W., Yan X., Zhu X.;
RT "Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is
RT essential for ciliary beat.";
RL J. Mol. Cell Biol. 11:383-394(2019).
CC -!- FUNCTION: Acts as a negative regulator of cell migration, invasion, and
CC metastasis downstream of p53/TP53, through inhibition of Arp2/3
CC complex-mediated actin polymerization (By similarity). Via its
CC association with the multisubunit axonemal dynein complex, is
CC potentially involved in the regulation of cilia function
CC (PubMed:30060180). May play a role in osteogenesis of dental tissue-
CC derived mesenchymal stem cells (PubMed:25498833).
CC {ECO:0000250|UniProtKB:Q8IWG1, ECO:0000269|PubMed:25498833,
CC ECO:0000269|PubMed:30060180}.
CC -!- SUBUNIT: Interacts with ACTR2; this interaction reduces binding of the
CC Arp2/3 complex to the VCA domain of nucleation promoting factors (By
CC similarity). Part of the multisubunit axonemal dynein complex formed at
CC least of two heavy chains and a number of intermediate and light chains
CC (PubMed:30060180). Found in a associated with the catalytic heavy chain
CC DNAH2, the intermediate chain DNAI4, and the light chain DYNLT1
CC (PubMed:30060180). {ECO:0000250|UniProtKB:Q8IWG1,
CC ECO:0000269|PubMed:30060180}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8IWG1}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in the testes (PubMed:26501274).
CC Detected also in brain and lung tissues (PubMed:26501274).
CC {ECO:0000269|PubMed:26501274}.
CC -!- DISRUPTION PHENOTYPE: Deficient mice shown no overt abnormalities in
CC body size, development, behavior, or fertility.
CC {ECO:0000269|PubMed:26501274}.
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DR EMBL; AC161212; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC158119; AAI58120.1; -; mRNA.
DR CCDS; CCDS17899.2; -.
DR RefSeq; NP_766452.2; NM_172864.3.
DR AlphaFoldDB; B2RY71; -.
DR STRING; 10090.ENSMUSP00000124475; -.
DR PhosphoSitePlus; B2RY71; -.
DR MaxQB; B2RY71; -.
DR PaxDb; B2RY71; -.
DR PRIDE; B2RY71; -.
DR ProteomicsDB; 341267; -.
DR Antibodypedia; 33558; 30 antibodies from 14 providers.
DR DNASU; 242253; -.
DR Ensembl; ENSMUST00000160285; ENSMUSP00000124475; ENSMUSG00000043020.
DR GeneID; 242253; -.
DR KEGG; mmu:242253; -.
DR UCSC; uc008rqw.2; mouse.
DR CTD; 126820; -.
DR MGI; MGI:3045269; Dnai3.
DR VEuPathDB; HostDB:ENSMUSG00000043020; -.
DR eggNOG; KOG1587; Eukaryota.
DR GeneTree; ENSGT00940000156924; -.
DR HOGENOM; CLU_009390_1_0_1; -.
DR InParanoid; B2RY71; -.
DR OMA; ANRRCES; -.
DR OrthoDB; 202058at2759; -.
DR PhylomeDB; B2RY71; -.
DR TreeFam; TF326991; -.
DR BioGRID-ORCS; 242253; 2 hits in 71 CRISPR screens.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; B2RY71; protein.
DR Bgee; ENSMUSG00000043020; Expressed in seminiferous tubule of testis and 67 other tissues.
DR GO; GO:0005858; C:axonemal dynein complex; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0036156; C:inner dynein arm; IBA:GO_Central.
DR GO; GO:0071933; F:Arp2/3 complex binding; ISS:UniProtKB.
DR GO; GO:0045504; F:dynein heavy chain binding; IBA:GO_Central.
DR GO; GO:0045503; F:dynein light chain binding; IBA:GO_Central.
DR GO; GO:0060294; P:cilium movement involved in cell motility; IBA:GO_Central.
DR GO; GO:0036159; P:inner dynein arm assembly; IBA:GO_Central.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR GO; GO:0034316; P:negative regulation of Arp2/3 complex-mediated actin nucleation; ISS:UniProtKB.
DR GO; GO:0030336; P:negative regulation of cell migration; ISS:UniProtKB.
DR GO; GO:0045669; P:positive regulation of osteoblast differentiation; IDA:UniProtKB.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..923
FT /note="Dynein axonemal intermediate chain 3"
FT /id="PRO_0000450864"
FT REPEAT 398..438
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 480..536
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 702..741
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 745..785
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 869..889
FT /evidence="ECO:0000255"
SQ SEQUENCE 923 AA; 105518 MW; 8CB13ECD97C37190 CRC64;
MAPKPPKSPK GQKKGKKNMK QQLLVPEEEE PMNMESMGHP EIYPLVLTTK TQEIFNCRVD
EDMTDEQTYK LIKKEDILAD LQNRAAVSDF HPVKKIVREY PGEELLLVYD KDFKYGLNFY
LIGTEDGKEN FLKPPEVPEE QEEPKEHVQE DVYVYNPPVS KPWVSLGSEK EIEEESVQES
GKRVTYMISR KRSEFGAPVT FSDQNASSVK DAYIECTSYP EKNYTLSQVE KDVGLQVIAE
VKDTSTQTKW AFPKNATTQY YPREFSEEEK SLIGKSKSLV DFFNNVSTSV EVALQQNEIM
NTFIDDWKNL AEEESTFGDK TDTHLKEYQS FTDLHNTMEK MITCVSWHPT IFGLIVVSVA
VRLSYEERVQ NSGRLLLQPS LLLFWSFSDP IHPQLMLESP DDIFCFEFCP SDPNIIAGGC
INGQIVLWDI TAHADRIENI KTGGHRSKKT SLKPMFLLEP DSNKESMYIR HCAVSSIENG
HRKVITDIHW LPDSFEINRM GSVFENRSGI NCQLVTCSAD CTICFWDIRP QKPAVTAAAA
ASAATTATNN ANSQQSPVEK KKEENIDIPF DVPSTFLHLD LSWKPLSRLK LSKGDTSLDH
CPTKLSLGED PFLCKIQGTS SIHIILRDKM LSQIKMVKTS EINPYQNLEA GIANILKPIE
DFCTKFFVGT EEGEVIYTDW KMERDSDTGR LMAKKPVSLY TVHDGAVHTI QRSPFFNDIV
LTVGGWNVAI WKEEVMTGPL LQTCCGPKRY TAGHWSLTRP GVFYIGREDG NVDIWDLLEK
THEPAQSQNI CITMITYIKP WTFSSKQQFI AVADYYGTLH ILEIPWTLSH PSLNEVSSVN
YYFEREVRHL EYVQQRKEIR EQEKIDMALE LVKKKAKIYQ KTKEQMEAEL KLEYESYLDL
EKSVLFALGL SKVSEKKSYL DSH
