DNAI4_DANRE
ID DNAI4_DANRE Reviewed; 778 AA.
AC F1QHZ6; Q502N3;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Dynein axonemal intermediate chain 4 {ECO:0000305};
DE AltName: Full=WD repeat-containing protein 78;
GN Name=dnai4 {ECO:0000312|ZFIN:ZDB-GENE-050522-322}; Synonyms=wdr78;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Olfactory epithelium;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=30060180; DOI=10.1093/jmcb/mjy043;
RA Zhang Y., Chen Y., Zheng J., Wang J., Duan S., Zhang W., Yan X., Zhu X.;
RT "Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is
RT essential for ciliary beat.";
RL J. Mol. Cell Biol. 11:383-394(2019).
CC -!- FUNCTION: Plays a critical role in the assembly of axonemal dynein
CC complex (By similarity). Plays a key role in ciliary motility
CC (PubMed:30060180). {ECO:0000250|UniProtKB:E9PYY5,
CC ECO:0000269|PubMed:30060180}.
CC -!- SUBUNIT: Part of the multisubunit axonemal dynein complex formed at
CC least of two heavy chains and a number of intermediate and light
CC chains. {ECO:0000250|UniProtKB:E9PYY5}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum axoneme
CC {ECO:0000250|UniProtKB:E9PYY5}. Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000250|UniProtKB:E9PYY5}. Dynein axonemal particle
CC {ECO:0000250|UniProtKB:Q6GPB9}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown results in ciliopathy-
CC related phenotypes, such as curved bodies, hydrocephalus, abnormal
CC otolith, randomized left-right asymmetry, and pronephric cysts,
CC accompanied with paralyzed pronephric cilia.
CC {ECO:0000269|PubMed:30060180}.
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DR EMBL; BX088595; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC095632; AAH95632.1; -; mRNA.
DR RefSeq; NP_001018595.1; NM_001020759.1.
DR AlphaFoldDB; F1QHZ6; -.
DR SMR; F1QHZ6; -.
DR STRING; 7955.ENSDARP00000065205; -.
DR PaxDb; F1QHZ6; -.
DR Ensembl; ENSDART00000065206; ENSDARP00000065205; ENSDARG00000044400.
DR Ensembl; ENSDART00000181971; ENSDARP00000146535; ENSDARG00000109570.
DR GeneID; 553797; -.
DR KEGG; dre:553797; -.
DR CTD; 79819; -.
DR ZFIN; ZDB-GENE-050522-322; dnai4.
DR eggNOG; KOG1587; Eukaryota.
DR GeneTree; ENSGT00940000156209; -.
DR HOGENOM; CLU_015820_0_0_1; -.
DR InParanoid; F1QHZ6; -.
DR OMA; WRYRDFR; -.
DR OrthoDB; 704698at2759; -.
DR PhylomeDB; F1QHZ6; -.
DR TreeFam; TF300553; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 6.
DR Bgee; ENSDARG00000044400; Expressed in testis and 8 other tissues.
DR GO; GO:0005858; C:axonemal dynein complex; ISS:UniProtKB.
DR GO; GO:0005930; C:axoneme; ISS:UniProtKB.
DR GO; GO:0120293; C:dynein axonemal particle; ISS:UniProtKB.
DR GO; GO:0031514; C:motile cilium; ISS:UniProtKB.
DR GO; GO:0045504; F:dynein heavy chain binding; IBA:GO_Central.
DR GO; GO:0045503; F:dynein light chain binding; IBA:GO_Central.
DR GO; GO:0070286; P:axonemal dynein complex assembly; ISS:UniProtKB.
DR GO; GO:0003341; P:cilium movement; IMP:ZFIN.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Cell projection; Cilium; Cytoplasm; Cytoskeleton; Flagellum;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..778
FT /note="Dynein axonemal intermediate chain 4"
FT /id="PRO_0000450826"
FT REPEAT 477..517
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 526..573
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 586..629
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 633..673
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 676..715
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 721..760
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT CONFLICT 56
FT /note="V -> L (in Ref. 2; AAH95632)"
FT /evidence="ECO:0000305"
FT CONFLICT 70
FT /note="M -> V (in Ref. 2; AAH95632)"
FT /evidence="ECO:0000305"
FT CONFLICT 211
FT /note="V -> L (in Ref. 2; AAH95632)"
FT /evidence="ECO:0000305"
FT CONFLICT 251
FT /note="S -> P (in Ref. 2; AAH95632)"
FT /evidence="ECO:0000305"
FT CONFLICT 305
FT /note="S -> P (in Ref. 2; AAH95632)"
FT /evidence="ECO:0000305"
FT CONFLICT 432
FT /note="S -> T (in Ref. 2; AAH95632)"
FT /evidence="ECO:0000305"
FT CONFLICT 477
FT /note="H -> Y (in Ref. 2; AAH95632)"
FT /evidence="ECO:0000305"
FT CONFLICT 586
FT /note="R -> Q (in Ref. 2; AAH95632)"
FT /evidence="ECO:0000305"
FT CONFLICT 629
FT /note="E -> D (in Ref. 2; AAH95632)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 778 AA; 86391 MW; 048B7A00282B80D1 CRC64;
MSNTTVTQKR RTLKVAKSSK TLNVSSGVFR PNQSTTKSIN PLFSGKSFGS TGDSKVVDKI
SAQTPKHTAM VVDENGQDVT PHPLYNPDPG VLQSKQGKVF TALDTSLTTM TEFPSIAFQT
TNASLTGPFT RSFFGSASAS RISQTAESVT DETKDLLVKQ DLSNSISDIQ SHREEPKEQL
RENILDSTAN CYLSETETIW LLDIPAVSVS VESEDAEAVK ERNNAYTELC KNRQGNDKYV
ERSMQTFSDA SKTKEVQCDS ITMVDKAVMC TVWDMYDSFN NISDVSANTV VSSERHEATI
PESSSEGHLV HPKRSNQSLS VVSTVSTVSG SSTHIEKMAC VLPLDEEPDL QLILQSDKLK
QDLALMERVV LANVFQPKLA AYRQLPIIED PDCVQMVMEE ESWTEQSKNS HCPFLERLWD
FSCELTMGRN VSCMVWNKKN PDLLAVGYGQ VEFKNPNSGL VCCWSLKNPT WPDRVFHCES
GVTALDFSAS NANQLAVGMY DGTIAIYNVQ TSEQTPITDS SDCANLHTSP VWQLTWIDHE
DGLAADKGEI LVSVSSDGRI SKWIHYKSME CVDLMKLKIH DLQWKRHISS LTPGMCFDFH
PNDSKIYLVG TEEGHIHKCS SSYNEQYLES YKAHKRPVYK VTWSPFCSDV FLSCSSDWTI
QLWRQDLQIP VMGFTSGQRV VFDIMWSPHC ATVFGAVSEG KVEIWDLRVS SLDPTLVNWT
SPGVNPTALL FSPETDCVLV GDSEGQVTVY KLKNITAGGG SQGETLEDVI QSTLSSQH
