DNAI4_MOUSE
ID DNAI4_MOUSE Reviewed; 807 AA.
AC E9PYY5; Q8C0I9; Q8K042; Q8K111;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Dynein axonemal intermediate chain 4 {ECO:0000305};
DE AltName: Full=WD repeat-containing protein 78;
GN Name=Dnai4; Synonyms=Wdr78 {ECO:0000312|MGI:MGI:2385328};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC STRAIN=FVB/N; TISSUE=Eye, and Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION, SUBUNIT, INTERACTION WITH DYNLT1, AND TISSUE SPECIFICITY.
RX PubMed=30060180; DOI=10.1093/jmcb/mjy043;
RA Zhang Y., Chen Y., Zheng J., Wang J., Duan S., Zhang W., Yan X., Zhu X.;
RT "Vertebrate Dynein-f depends on Wdr78 for axonemal localization and is
RT essential for ciliary beat.";
RL J. Mol. Cell Biol. 11:383-394(2019).
CC -!- FUNCTION: Plays a critical role in the assembly of axonemal dynein
CC complex, thereby playing a role in ciliary motility.
CC {ECO:0000269|PubMed:30060180}.
CC -!- SUBUNIT: Part of the multisubunit axonemal dynein complex formed at
CC least of two heavy chains and a number of intermediate and light
CC chains. Associated with axonemal dynein subunits such as, DNAH2, DNAI3,
CC and DYNLT1 (PubMed:30060180). Interacts with DYNLT1 (PubMed:30060180).
CC {ECO:0000269|PubMed:30060180}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, flagellum axoneme
CC {ECO:0000269|PubMed:30060180}. Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000269|PubMed:30060180}. Dynein axonemal particle
CC {ECO:0000250|UniProtKB:Q6GPB9}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=E9PYY5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=E9PYY5-2; Sequence=VSP_042401, VSP_042402;
CC Name=3;
CC IsoId=E9PYY5-3; Sequence=VSP_042399, VSP_042400, VSP_042403;
CC Name=4;
CC IsoId=E9PYY5-4; Sequence=VSP_042399, VSP_042403;
CC -!- TISSUE SPECIFICITY: Highly expressed in tissues containing motile
CC cilia, including the trachea, lung, oviduct, and testis.
CC {ECO:0000269|PubMed:30060180}.
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DR EMBL; AK030997; BAC27209.1; -; mRNA.
DR EMBL; AL844176; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC028975; AAH28975.1; -; mRNA.
DR EMBL; BC034130; AAH34130.1; -; mRNA.
DR CCDS; CCDS38824.3; -. [E9PYY5-1]
DR RefSeq; NP_666366.4; NM_146254.4. [E9PYY5-1]
DR AlphaFoldDB; E9PYY5; -.
DR SMR; E9PYY5; -.
DR STRING; 10090.ENSMUSP00000102481; -.
DR PhosphoSitePlus; E9PYY5; -.
DR jPOST; E9PYY5; -.
DR PaxDb; E9PYY5; -.
DR PRIDE; E9PYY5; -.
DR ProteomicsDB; 275211; -. [E9PYY5-1]
DR ProteomicsDB; 275212; -. [E9PYY5-2]
DR ProteomicsDB; 275213; -. [E9PYY5-3]
DR ProteomicsDB; 275214; -. [E9PYY5-4]
DR Antibodypedia; 51698; 17 antibodies from 8 providers.
DR DNASU; 242584; -.
DR Ensembl; ENSMUST00000036451; ENSMUSP00000037588; ENSMUSG00000035126. [E9PYY5-2]
DR Ensembl; ENSMUST00000036557; ENSMUSP00000042272; ENSMUSG00000035126. [E9PYY5-3]
DR Ensembl; ENSMUST00000106868; ENSMUSP00000102481; ENSMUSG00000035126. [E9PYY5-1]
DR Ensembl; ENSMUST00000116316; ENSMUSP00000112018; ENSMUSG00000035126. [E9PYY5-2]
DR GeneID; 242584; -.
DR KEGG; mmu:242584; -.
DR UCSC; uc029uyj.1; mouse. [E9PYY5-1]
DR CTD; 79819; -.
DR MGI; MGI:2385328; Dnai4.
DR VEuPathDB; HostDB:ENSMUSG00000035126; -.
DR eggNOG; ENOG502QWGI; Eukaryota.
DR GeneTree; ENSGT00940000156209; -.
DR HOGENOM; CLU_868662_0_0_1; -.
DR InParanoid; E9PYY5; -.
DR OMA; WRYRDFR; -.
DR OrthoDB; 704698at2759; -.
DR PhylomeDB; E9PYY5; -.
DR TreeFam; TF300553; -.
DR BioGRID-ORCS; 242584; 1 hit in 66 CRISPR screens.
DR ChiTaRS; Wdr78; mouse.
DR PRO; PR:E9PYY5; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; E9PYY5; protein.
DR Bgee; ENSMUSG00000035126; Expressed in spermatid and 180 other tissues.
DR ExpressionAtlas; E9PYY5; baseline and differential.
DR Genevisible; E9PYY5; MM.
DR GO; GO:0005858; C:axonemal dynein complex; IMP:UniProtKB.
DR GO; GO:0005930; C:axoneme; IDA:UniProtKB.
DR GO; GO:0120293; C:dynein axonemal particle; ISS:UniProtKB.
DR GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR GO; GO:0045504; F:dynein heavy chain binding; IBA:GO_Central.
DR GO; GO:0045503; F:dynein light chain binding; IBA:GO_Central.
DR GO; GO:0070286; P:axonemal dynein complex assembly; IMP:UniProtKB.
DR GO; GO:0003341; P:cilium movement; IMP:UniProtKB.
DR GO; GO:0002244; P:hematopoietic progenitor cell differentiation; IMP:MGI.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 2.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium; Cytoplasm; Cytoskeleton;
KW Flagellum; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..807
FT /note="Dynein axonemal intermediate chain 4"
FT /id="PRO_0000415828"
FT REPEAT 493..533
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 542..590
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 617..657
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 661..701
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 704..743
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 749..788
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 300..320
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..323
FT /note="Missing (in isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_042399"
FT VAR_SEQ 458..486
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_042400"
FT VAR_SEQ 590..611
FT /note="DLMRLKRTTATGGKKGGEKEKK -> GHEHLSGWYRRRPYSQVFLFIQ (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_042401"
FT VAR_SEQ 612..807
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_042402"
FT VAR_SEQ 664..807
FT /note="GPVYKVTWNPFCPDVFLSCSADWGVMIWHQDTVKPFLSFYPTTYVVYDVSWS
FT PKSAYIFAAANENRVEIWDLQISTLDPLIVNVANPGIKFTTVLFAKETDCLLVGDSDGQ
FT VAVYELRNMPTASDTSRGDVINILLGPKTNHTG -> FGPSDRECC (in isoform
FT 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_042403"
SQ SEQUENCE 807 AA; 90173 MW; 45C5F65A9CAE7010 CRC64;
MHSSPTSTRK QASFAASASA QPRKSISFIN PSKSSAGKGY AASNPNKLAV SRTMGFLTDM
KPAEKLNVPS AKTVQVLDSK GVDVTPRPLY HPDPHAASAK PNKLLTSQEG SLGSDYISSY
SLYQNTLNPS MLGQYTRSVL GSSSVSKSSI STTESMSEDL EDSSYKRDRL ASFTDVRVLR
STAEAAISKE ELEKTIEIIL TETETLRFFD LPTVMFSTES EEAEKIIEKN KKYETLCRNR
LGNDLYVERM MQTFNGAPKN KEVQCEKIIL EEKGVVATTW DLYDSYNIPE TLLAAKRSGY
SSKGSLPAKD RDPKIQDSES SSLMDIENVI LAKVQEDEED NSEAILKSDK LHQDLFYMER
VLMENVFQPK LAAYRQLPVY KEHEPEEPEE TLQVENLKVA EDEPKKEDEE EVEMELELEI
ATEQSTIPAN LERLWSFSCD LTKGLNVSSL SWNKANPDLL AVGYGNFGFR EQKKGMACCW
SIKNPMWPER IYQSSYGVTS VDFSNSSPNL LAVGYHNGTV AIYNVQSSHN IPVLDSSESP
QKHLGPVWQV QWIEQDRGTT GDDKREILVS ISADGRISKW IIRKGLDCHD LMRLKRTTAT
GGKKGGEKEK KGEALISRQA PGMCFAFHPK DTNIYLAGTE EGLIHKCSCS YNEQYLETYR
GHKGPVYKVT WNPFCPDVFL SCSADWGVMI WHQDTVKPFL SFYPTTYVVY DVSWSPKSAY
IFAAANENRV EIWDLQISTL DPLIVNVANP GIKFTTVLFA KETDCLLVGD SDGQVAVYEL
RNMPTASDTS RGDVINILLG PKTNHTG
