DNAI_BACSU
ID DNAI_BACSU Reviewed; 311 AA.
AC P06567;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Primosomal protein DnaI;
GN Name=dnaI; Synonyms=ytxA; OrderedLocusNames=BSU28980;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3027671; DOI=10.1093/nar/14.24.9989;
RA Ogasawara N., Moriya S., Mazza P.G., Yoshikawa H.;
RT "Nucleotide sequence and organization of dnaB gene and neighbouring genes
RT on the Bacillus subtilis chromosome.";
RL Nucleic Acids Res. 14:9989-9999(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969504; DOI=10.1099/13500872-142-11-3067;
RA Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J.,
RA Emmerson P.T., Harwood C.R.;
RT "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis
RT chromosome containing genes responsible for stress responses, the
RT utilization of plant cell walls and primary metabolism.";
RL Microbiology 142:3067-3078(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT 200 kb rrnB-dnaB region.";
RL Microbiology 143:3431-3441(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-206.
RX PubMed=3027697; DOI=10.1073/pnas.84.3.653;
RA Hoshino T., McKenzie T., Schmidt S., Tanaka T., Sueoka N.;
RT "Nucleotide sequence of Bacillus subtilis dnaB: a gene essential for DNA
RT replication initiation and membrane attachment.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:653-657(1987).
RN [6]
RP SIMILARITY TO DNAA.
RX PubMed=1549481; DOI=10.1093/nar/20.5.1143;
RA Koonin E.V.;
RT "Archaebacterial virus SSV1 encodes a putative DnaA-like protein.";
RL Nucleic Acids Res. 20:1143-1143(1992).
RN [7]
RP IDENTIFICATION.
RX PubMed=7773414; DOI=10.1099/13500872-141-5-1199;
RA Bruand C., Ehrlich S.D.;
RT "The Bacillus subtilis dnaI gene is part of the dnaB operon.";
RL Microbiology 141:1199-1200(1995).
CC -!- FUNCTION: Probably involved in DNA replication.
CC -!- INTERACTION:
CC P06567; P37469: dnaC; NbExp=4; IntAct=EBI-2122847, EBI-2122822;
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DR EMBL; X04963; CAA28633.1; -; Genomic_DNA.
DR EMBL; Z75208; CAA99605.1; -; Genomic_DNA.
DR EMBL; AF008220; AAC00359.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14858.1; -; Genomic_DNA.
DR EMBL; M15183; AAA22405.1; -; Genomic_DNA.
DR PIR; B24720; IQBS44.
DR RefSeq; NP_390776.1; NC_000964.3.
DR RefSeq; WP_003229466.1; NZ_JNCM01000036.1.
DR PDB; 2K7R; NMR; -; A=1-106.
DR PDB; 4M4W; X-ray; 6.10 A; J/K/L/M/N/O=1-311.
DR PDBsum; 2K7R; -.
DR PDBsum; 4M4W; -.
DR AlphaFoldDB; P06567; -.
DR BMRB; P06567; -.
DR SMR; P06567; -.
DR IntAct; P06567; 4.
DR STRING; 224308.BSU28980; -.
DR jPOST; P06567; -.
DR PaxDb; P06567; -.
DR PRIDE; P06567; -.
DR EnsemblBacteria; CAB14858; CAB14858; BSU_28980.
DR GeneID; 936873; -.
DR KEGG; bsu:BSU28980; -.
DR PATRIC; fig|224308.179.peg.3147; -.
DR eggNOG; COG1484; Bacteria.
DR OMA; HFTYSQR; -.
DR PhylomeDB; P06567; -.
DR BioCyc; BSUB:BSU28980-MON; -.
DR EvolutionaryTrace; P06567; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR GO; GO:0006271; P:DNA strand elongation involved in DNA replication; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR013317; DnaA.
DR InterPro; IPR009928; DnaI_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00308; Bac_DnaA; 1.
DR Pfam; PF07319; DnaI_N; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; DNA replication; Nucleotide-binding; Primosome;
KW Reference proteome.
FT CHAIN 1..311
FT /note="Primosomal protein DnaI"
FT /id="PRO_0000114313"
FT BINDING 168..175
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000305"
FT CONFLICT 19
FT /note="K -> N (in Ref. 5; AAA22405)"
FT /evidence="ECO:0000305"
FT CONFLICT 24
FT /note="M -> T (in Ref. 5; AAA22405)"
FT /evidence="ECO:0000305"
FT HELIX 16..28
FT /evidence="ECO:0007829|PDB:2K7R"
FT HELIX 32..40
FT /evidence="ECO:0007829|PDB:2K7R"
FT TURN 42..44
FT /evidence="ECO:0007829|PDB:2K7R"
FT HELIX 47..52
FT /evidence="ECO:0007829|PDB:2K7R"
FT HELIX 54..62
FT /evidence="ECO:0007829|PDB:2K7R"
FT TURN 68..70
FT /evidence="ECO:0007829|PDB:2K7R"
FT STRAND 83..90
FT /evidence="ECO:0007829|PDB:2K7R"
FT STRAND 93..100
FT /evidence="ECO:0007829|PDB:2K7R"
FT HELIX 102..104
FT /evidence="ECO:0007829|PDB:2K7R"
SQ SEQUENCE 311 AA; 36114 MW; A86FC94AB6841264 CRC64;
MEPIGRSLQG VTGRPDFQKR LEQMKEKVMK DQDVQAFLKE NEEVIDQKMI EKSLNKLYEY
IEQSKNCSYC SEDENCNNLL EGYHPKLVVN GRSIDIEYYE CPVKRKLDQQ KKQQSLMKSM
YIQQDLLGAT FQQVDISDPS RLAMFQHVTD FLKSYNETGK GKGLYLYGKF GVGKTFMLAA
IANELAEKEY SSMIVYVPEF VRELKNSLQD QTLEEKLNMV KTTPVLMLDD IGAESMTSWV
RDEVIGTVLQ HRMSQQLPTF FSSNFSPDEL KHHFTYSQRG EKEEVKAARL MERILYLAAP
IRLDGENRRH P
