ADDA_STRSV
ID ADDA_STRSV Reviewed; 1224 AA.
AC A3CNT9;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN Name=addA {ECO:0000255|HAMAP-Rule:MF_01451}; Synonyms=rexA;
GN OrderedLocusNames=SSA_1451;
OS Streptococcus sanguinis (strain SK36).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=388919;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SK36;
RX PubMed=17277061; DOI=10.1128/jb.01808-06;
RA Xu P., Alves J.M., Kitten T., Brown A., Chen Z., Ozaki L.S., Manque P.,
RA Ge X., Serrano M.G., Puiu D., Hendricks S., Wang Y., Chaplin M.D., Akan D.,
RA Paik S., Peterson D.L., Macrina F.L., Buck G.A.;
RT "Genome of the opportunistic pathogen Streptococcus sanguinis.";
RL J. Bacteriol. 189:3166-3175(2007).
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddA nuclease domain is
CC required for chi fragment generation; this subunit has the helicase and
CC 3' -> 5' nuclease activities. {ECO:0000255|HAMAP-Rule:MF_01451}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC Rule:MF_01451}.
CC -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01451}.
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DR EMBL; CP000387; ABN44844.1; -; Genomic_DNA.
DR RefSeq; WP_011837144.1; NC_009009.1.
DR RefSeq; YP_001035394.1; NC_009009.1.
DR AlphaFoldDB; A3CNT9; -.
DR SMR; A3CNT9; -.
DR STRING; 388919.SSA_1451; -.
DR EnsemblBacteria; ABN44844; ABN44844; SSA_1451.
DR KEGG; ssa:SSA_1451; -.
DR PATRIC; fig|388919.9.peg.1376; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_001114_3_1_9; -.
DR OMA; KQSIYRW; -.
DR OrthoDB; 137860at2; -.
DR Proteomes; UP000002148; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 4.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01451; AddA; 1.
DR InterPro; IPR014152; DNA_helicase_suAddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR PANTHER; PTHR11070:SF48; PTHR11070:SF48; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
DR TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Exonuclease; Helicase;
KW Hydrolase; Nuclease; Nucleotide-binding; Reference proteome.
FT CHAIN 1..1224
FT /note="ATP-dependent helicase/nuclease subunit A"
FT /id="PRO_0000379351"
FT DOMAIN 26..491
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT DOMAIN 519..809
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT BINDING 47..54
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
SQ SEQUENCE 1224 AA; 139912 MW; 6E7DFE103418B867 CRC64;
MKRIPFLTAE EIALKQAQEA ASDKPQKKTA EQIEAIYSSG RNILVSASAG SGKTFVMVQR
IIDQILRGVA VSQLFISTFT VKAAGELKER LEKELGQALK EAESPELKQH LAQQLADLPN
ADIGTMDSFT QKVLSRYGYL LGLAPNFRIL QSASEQLILQ NEVFSQVFDH YYDSERQALF
SRLVKNFTGK RKDLSAFREQ VYRIYSFLQS TSSPQRWLEE TFLYGYEHSD FAAERERIFC
QIKSALWELE TFFSAHLEHE GREFAGAKYQ ENVQDALTVL AGLNESSSIE ETAQILKQIV
ALSQLSNGQA FTARVGKNAD ELKKEMAKDY NEARKPMIER LRSFDQQLYQ LDFIEQHQDE
CLPLVELLRD FVADFAQAYL ERKKAENAFE FGDISHFAIE ILETFPEVRR FYQERYHEVM
VDEYQDTNHT QERMLDLLSR GKNRFMVGDI KQSIYRFRQA DPQIFSDKFK AYQEDSSQGK
LIVLKENFRS HLEVLEATND VFKRLMDEEV GEIDYNETHY LVAGNPAKRE PNPANRASFL
IYEGSKESPE EEADEGLPQA VSAGEVDLVI KEIIRLHNEE GVAFKDITLL TASRTRNDLI
LAAFEQHQIP LVPDDGAANY LQSVEVLVML DTLRTINNPL NDYALTALLK SPMFDFGEDE
LARLSLQASQ ERSQENLYEK LVNALEGRGL NPALVTEELQ KKLQHFYETL QSWRTYSKTH
SLYDLIWKIY QDRFYYDMVG TLVNGAQRQA NLYALSLRAN EYEKSSFKGL SRFIGMIDRI
LENQHDLASV PVAAPKDAVR LMTIHKSKGL EFKYVFLLNM DKAFNRQDSS SAIILSRTKG
VGIKYVADVS VSVKDSYAPN QLRISMDTLP YQQNLAELQL ASLSEQMRLL YVAMTRAETK
LYLVGKGSQE ALDKRQWGKS QQGRLSASLR SQISNFQDWL YAIQDVFSDE NLAYETRFVT
DEELTAEEIG RINEPVLFPA DDLADNRQSD DIRRALDILE SVDRLNSQYR SAIELPSVRT
PSQIKKFYEP IMDTDGLDIM DERAAFRPQP SFELPDFGKK AKVTGAQVGS AVHELMQRIP
LDSSPSMAVL RSALAQVQAD DAVKKQIQLS KIASFFETDL GRLLIENSDR VRREAPFAML
KRDEASGQEF VLRGILDGYL LFEDRIILFD YKTDKYKDSS ELIARYRGQL DLYAQALSRS
YGISQIEKYL ILLGGEQLQV VKVD
