ADDA_SYNWW
ID ADDA_SYNWW Reviewed; 1236 AA.
AC Q0AXU8;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN Name=addA {ECO:0000255|HAMAP-Rule:MF_01451}; OrderedLocusNames=Swol_1146;
OS Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Syntrophomonadaceae;
OC Syntrophomonas.
OX NCBI_TaxID=335541;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 2245B / Goettingen;
RX PubMed=21966920; DOI=10.1111/j.1462-2920.2010.02237.x;
RA Sieber J.R., Sims D.R., Han C., Kim E., Lykidis A., Lapidus A.L.,
RA McDonnald E., Rohlin L., Culley D.E., Gunsalus R., McInerney M.J.;
RT "The genome of Syntrophomonas wolfei: new insights into syntrophic
RT metabolism and biohydrogen production.";
RL Environ. Microbiol. 12:2289-2301(2010).
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddA nuclease domain is
CC required for chi fragment generation; this subunit has the helicase and
CC 3' -> 5' nuclease activities. {ECO:0000255|HAMAP-Rule:MF_01451}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC Rule:MF_01451}.
CC -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01451}.
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DR EMBL; CP000448; ABI68456.1; -; Genomic_DNA.
DR RefSeq; WP_011640560.1; NC_008346.1.
DR AlphaFoldDB; Q0AXU8; -.
DR SMR; Q0AXU8; -.
DR STRING; 335541.Swol_1146; -.
DR EnsemblBacteria; ABI68456; ABI68456; Swol_1146.
DR KEGG; swo:Swol_1146; -.
DR eggNOG; COG1074; Bacteria.
DR HOGENOM; CLU_001114_3_1_9; -.
DR OMA; KQSIYRW; -.
DR OrthoDB; 137860at2; -.
DR Proteomes; UP000001968; Chromosome.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 4.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01451; AddA; 1.
DR InterPro; IPR014152; DNA_helicase_suAddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR PANTHER; PTHR11070:SF48; PTHR11070:SF48; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Exonuclease; Helicase;
KW Hydrolase; Nuclease; Nucleotide-binding; Reference proteome.
FT CHAIN 1..1236
FT /note="ATP-dependent helicase/nuclease subunit A"
FT /id="PRO_0000379359"
FT DOMAIN 2..457
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT DOMAIN 515..816
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
FT BINDING 23..30
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01451"
SQ SEQUENCE 1236 AA; 143767 MW; 0ECBB039DED9E286 CRC64;
MAHWTIEQEE AINARNSNLL VAAAAGSGKT TVLVERIIQL VLRDRIDIDR LLIVTFTQAA
AGEMRERINA AFFKELEKGR EDGHLRRQLY LLNRSSISTI HAFCSDVVRQ HFHLVNIDPH
FRIADSTETE LIKMEVLEEL LDGEYEKGND GFLDLVEAFG SNKDDKPLEA LILRLHSFIQ
SHPQPLSWLE EKIDNLALGE DNWAENPWAS ELSQQIKIEL SAAQDILNQA LKLSNKAGGP
RGYLEAIESD QKWVELLLKA ADQGLPVLYS CLQQLSFTRL GRVSRDVDEN LKNQVKILRD
ESKKILNAIN SLLGRDPLEY LQDLNEIYSL MKYLGEIIQS FAEIYQEKKR EKGIVDFNDL
EHLALQILSN EAVAREYRDY YSYLFIDEYQ DSNLVQETIL NYIKKEDNLF MVGDVKQSIY
RFRLADPSLF LEKQKSYPLQ DGSVNRRVDL NKNFRSHPEI LNAVNYIFRH LMSEELGEID
YDEKSYLYPG LNTGQELKYQ EIDKASSQEG KEKENTAKRV EICILENNPD LITKLEENET
EEREIPGEAE NDFIERIIEM DNTEIEALLI AQKIRQLIQE DIYDPELQCM RKIEYRDMVI
LLRATRNSAG IFMEQLSAEG IPVYADASSG YFDTLELNLF INLLRLIDNK RQDIALLSVM
RSPIGGFSID DFIKIRTKLI PRREKQPYSF YEAMEFYMED NNDDLKDRLV FFIQRLKEWE
LESRIMALDE FMGKLFMDTG YYYYAGAMPG GGQRQANLRI LLHRAREFQK SSFKGLFSFI
KYIEKIKSSG SDMGNACIIG ENDNVVRIMS IHKSKGLEFP VVILGGLGKN FNIRDSNENV
LLHRKLGIGP RYINTQLRTY HDTIARLAIK NRIKLENLAE EMRILYVACT RPQEKLIMVG
TTRQLESAWR RWSQPVNSYN QSRARSFLDW LIPIIMRHKK EGQYLREIAG GDWDREILWE
DESRWKVKLI SPWQLTAEEI RKKEEKYEWE RLLEQGGYSC PSAESEEIIK RLNWKYPYQE
AENIPAKLSV SQVSQISSGY LEDGAADTFL TRVPAFLSGE KDKKTRLSPA DKGSIVHLVM
QNIDYRRVSR EDSINQQLGE MVEREILTSE QLAVVEVNKI WRFFQTKLGQ RVLQAKWLFR
EAPFNLLIAA SEVFPALESQ EELLIQGIID LYFYEGEDIV LLDFKSDIVH HKSEEEILAP
YRVQLKLYKR ALENITDHRV KESYLYFFDL NRAIRV
