ADDB_CALS4
ID ADDB_CALS4 Reviewed; 1159 AA.
AC Q8RCZ1;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B {ECO:0000255|HAMAP-Rule:MF_01452};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01452};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01452};
DE AltName: Full=ATP-dependent helicase/nuclease AddB {ECO:0000255|HAMAP-Rule:MF_01452};
GN Name=addB {ECO:0000255|HAMAP-Rule:MF_01452}; OrderedLocusNames=TTE0263;
OS Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM
OS 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Caldanaerobacter.
OX NCBI_TaxID=273068;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15242 / JCM 11007 / NBRC 100824 / MB4;
RX PubMed=11997336; DOI=10.1101/gr.219302;
RA Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y.,
RA Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R.,
RA Wang J., Yu J., Yang H.;
RT "A complete sequence of the T. tengcongensis genome.";
RL Genome Res. 12:689-700(2002).
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddB nuclease domain is not
CC required for chi fragment generation; this subunit has 5' -> 3'
CC nuclease activity. {ECO:0000255|HAMAP-Rule:MF_01452}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- SUBUNIT: Heterodimer of AddA and AddB. {ECO:0000255|HAMAP-
CC Rule:MF_01452}.
CC -!- SIMILARITY: Belongs to the helicase family. AddB/RexB type 1 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01452}.
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DR EMBL; AE008691; AAM23559.1; -; Genomic_DNA.
DR RefSeq; WP_011024732.1; NC_003869.1.
DR AlphaFoldDB; Q8RCZ1; -.
DR SMR; Q8RCZ1; -.
DR STRING; 273068.TTE0263; -.
DR EnsemblBacteria; AAM23559; AAM23559; TTE0263.
DR KEGG; tte:TTE0263; -.
DR eggNOG; COG3857; Bacteria.
DR HOGENOM; CLU_007838_0_0_9; -.
DR OMA; DRLENYV; -.
DR OrthoDB; 1283891at2; -.
DR Proteomes; UP000000555; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0008409; F:5'-3' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 4.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01452; AddB_type1; 1.
DR InterPro; IPR014140; DNA_helicase_suAddB.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02773; addB_Gpos; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; ATP-binding; DNA damage; DNA repair; Exonuclease; Hydrolase; Iron;
KW Iron-sulfur; Metal-binding; Nuclease; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..1159
FT /note="ATP-dependent helicase/deoxyribonuclease subunit B"
FT /id="PRO_0000379226"
FT REGION 1140..1159
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 8..15
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 784
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1102
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1105
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1111
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
SQ SEQUENCE 1159 AA; 135216 MW; 35B36BA20D413B1E CRC64;
MSIRFIYGRA GSGKTYFCLE EIKHKLNDGA NHPLILLVPE QFTFEAEKYL LDMIERDEKM
RAQVLSFKTL ANRVFVEVGG LARQHMKACG KSMVIYKVLE ENKEKLKVYS KASRQQGFVK
KISEAITEFK RFDVTPFQLI DASEKIEKLG LKEKLEDLAL IYSSFEEVLH KNYIDEEDEL
DLLSKKLEKS LQFEGAEFWI DGFTGFTPKQ YKVIEKLLKK ASRVSVTLTL DPSIDSIDPT
HLFYTTKKTE EKLIKICETN GISVEEPVNL NKGIPKRFEH NKELAFLEKN FFSHPYEIYN
EETKNISIFK ATNMYSEVEE VARDIARLIR DEHMRYSDIV VATKDLKRYY KLVKAIFSHY
GIPHFIDLKI NITNNPIIVY VISIFEIYLK NWSYESVFRY LKTGFTGIDK EEINLLENYV
LANGIKGNKW KERWEYRIDY KTDSLLMEER EKQIINKVNE VRERVYLPLE KFYTRFSHSK
NVKEACEVLY DFLVENKLPE KIEKFIEEFK NRGEFDTANQ YAQIWDIVVD VLDQMVEVLG
EEKISLEQFA RLISIGFDEY QIASIPPALD EVLVTSVDRM KSHNSKVLYL LGANDGVFPA
SSFEEGIFSD EERNLLSSLD LELDRDTKAK VFEEQFLVYT ALTSASEFLK ISYPIADHEG
RSLRPSIIIS RLRRIFPKIK VSTNIVEMDT DEENLNRVTV PLPTFNEMIK SFKKWNITGK
IHPIWLEVYK WYRTKDEWKK KLEDTLEGFV YDNQIKRIPP LKIKKLYGEE MEFSVSRLEK
YAACPFAYFV QYGLKAKERK IYGFEPPDLG IFMHNVLNEI AKALEKEELT WQEIDKEWCN
DAVDIIVEEM VDKIPGYILK SSSRYRYLAN RLKRVLSKAV WIISEHMKRS SFVPLGHEVA
FGENQKYPPI KIVLSNGEEI KLIGRIDRVD VLEKEGETYV RIIDYKSGDK TLDLSDVLYG
LELQLLVYLD AILESAFEGK ANLSPAGIFY FKIDDPIVRA DKDISDEELY KEIMKRLRLE
GFVLKSLDII REMDKLIEGT SYVIPASINK DGTIGKNTKG LTEEQFEILR KFVKKKSKKL
AEEMLQGDIS ILPYKKEKET ACQYCPYSSI CKFETNFKGN DYRRIESKEE KLWSIFEEEV
KEDGSQVDGR TEGSDNNEG
