ID   DNAK_ACIF2              Reviewed;         634 AA.
AC   B7J7X9;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Chaperone protein DnaK {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=HSP70 {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock 70 kDa protein {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock protein 70 {ECO:0000255|HAMAP-Rule:MF_00332};
GN   Name=dnaK {ECO:0000255|HAMAP-Rule:MF_00332}; OrderedLocusNames=AFE_2665;
OS   Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / CIP 104768
OS   / NCIMB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)).
OC   Bacteria; Proteobacteria; Acidithiobacillia; Acidithiobacillales;
OC   Acidithiobacillaceae; Acidithiobacillus.
OX   NCBI_TaxID=243159;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23270 / DSM 14882 / CIP 104768 / NCIMB 8455;
RX   PubMed=19077236; DOI=10.1186/1471-2164-9-597;
RA   Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II,
RA   Eisen J.A., Holmes D.S.;
RT   "Acidithiobacillus ferrooxidans metabolism: from genome sequence to
RT   industrial applications.";
RL   BMC Genomics 9:597-597(2008).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000255|HAMAP-Rule:MF_00332}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock. {ECO:0000255|HAMAP-
CC       Rule:MF_00332}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00332}.
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DR   EMBL; CP001219; ACK80714.1; -; Genomic_DNA.
DR   RefSeq; WP_009563301.1; NC_011761.1.
DR   AlphaFoldDB; B7J7X9; -.
DR   SMR; B7J7X9; -.
DR   STRING; 243159.AFE_2665; -.
DR   PaxDb; B7J7X9; -.
DR   EnsemblBacteria; ACK80714; ACK80714; AFE_2665.
DR   GeneID; 66433623; -.
DR   KEGG; afr:AFE_2665; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_1_6; -.
DR   OMA; ISIKRHM; -.
DR   OrthoDB; 161217at2; -.
DR   Proteomes; UP000001362; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 1.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Stress response.
FT   CHAIN           1..634
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_1000119656"
FT   REGION          601..634
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         199
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00332"
SQ   SEQUENCE   634 AA;  68222 MW;  888BB47971ADD4C9 CRC64;
     MAKVIGIDLG TTNSCVAVME GDKVKVIENS EGKRTTPSIV AITEEGEVLV GEAAKRQAVT
     NPENTVYEVK RLIGRKFDDA EVQKDLKHVP YKVIKADNGD AWVEVRDKKY SAQQISAFIL
     QKMKKTAEDY LGEKVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAFG
     EDKKPGDSKI AVYDLGGGTF DISIIEIAEM EGEHQFEVLS TNGDTFLGGG DFDSRVINYL
     ADSFKAESGI DLRGDRLAMQ RLKEAAEKAK IELSSAQQTD VNLPFITADQ SGPKHLNMKL
     TRAKLESLVE DLIDRSMAPC RVAMKDANLA TSRITDVILV GGQSRMPKVQ EKVKDFFGQD
     PRKDVNPDEA VAIGAAIQGA VLSGEKKDVL LMDVTPLSLG IETLGGVMTK LIEKNTTIPT
     RKSQIFSTAE DNQSAVTVHV LQGERELARD NKSLARFDLT DIANAPRGMP QIEVTFDIDA
     NGILHVSAKD NQTGKEQSIK ITASSGLSEE EIKRMIQEAE AHAADDKKAR ALIEARNEAD
     ASVHGARKAV EEHAAAPEHD KTKVTEAISA VENAAKGEDV EAIKGAVATL MAAMSALLQS
     AAAGQAQAES GAGAQGNAKP DDVVDAEFEE VDKK