ADDB_CLOK1
ID ADDB_CLOK1 Reviewed; 1150 AA.
AC B9E0C6;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B {ECO:0000255|HAMAP-Rule:MF_01452};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01452};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01452};
DE AltName: Full=ATP-dependent helicase/nuclease AddB {ECO:0000255|HAMAP-Rule:MF_01452};
GN Name=addB {ECO:0000255|HAMAP-Rule:MF_01452}; OrderedLocusNames=CKR_0900;
OS Clostridium kluyveri (strain NBRC 12016).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=583346;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 12016;
RA Inui M., Nonaka H., Shinoda Y., Ikenaga Y., Abe M., Naito K., Vertes A.A.,
RA Yukawa H.;
RT "Complete genome sequence of Clostridium kluyveri and comparative genomics
RT of Clostridia species.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddB nuclease domain is not
CC required for chi fragment generation; this subunit has 5' -> 3'
CC nuclease activity. {ECO:0000255|HAMAP-Rule:MF_01452}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- SUBUNIT: Heterodimer of AddA and AddB. {ECO:0000255|HAMAP-
CC Rule:MF_01452}.
CC -!- SIMILARITY: Belongs to the helicase family. AddB/RexB type 1 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01452}.
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DR EMBL; AP009049; BAH05951.1; -; Genomic_DNA.
DR RefSeq; WP_012101368.1; NC_011837.1.
DR AlphaFoldDB; B9E0C6; -.
DR SMR; B9E0C6; -.
DR EnsemblBacteria; BAH05951; BAH05951; CKR_0900.
DR KEGG; ckr:CKR_0900; -.
DR HOGENOM; CLU_007838_0_0_9; -.
DR Proteomes; UP000007969; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0008409; F:5'-3' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 4.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01452; AddB_type1; 1.
DR InterPro; IPR014140; DNA_helicase_suAddB.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02773; addB_Gpos; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; ATP-binding; DNA damage; DNA repair; Exonuclease; Hydrolase; Iron;
KW Iron-sulfur; Metal-binding; Nuclease; Nucleotide-binding.
FT CHAIN 1..1150
FT /note="ATP-dependent helicase/deoxyribonuclease subunit B"
FT /id="PRO_0000379178"
FT BINDING 8..15
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 789
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1109
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1112
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1118
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
SQ SEQUENCE 1150 AA; 133639 MW; F9EE18D0E9A5A5CC CRC64;
MSIRFIYGRA GSGKSHYCLE DIKRRIQEVN DRNLILLVPE QFSFQAEKNL IKSIGEKGTL
KAQVLSFRRM AEKVFDEVGG GIKKYINDAG KNILLYKIIE ENKNKLKVYK TSAKKQGFVN
LVSDIIGEFK KYNISPEFLK ENLDSIENKS LKNKLEDIGM LFLEFQNRLS KNYIDLEDTL
HILCEKLDKS IIFKNSEVWI DEFSTFTPQE YSIIEKIMCS AYRVNITLCM DALGEYSERE
SIDLFLPIKI TERNILQIAE KNNIIYQKPV NLRCSPCYRF KNSTALQHLQ HSLFSYPYKN
YEKSTEDINI FKALNKYTEI DYIARDIVKA CRDKNLRFKD MAVVTGDLDE YENLIKAVFN
QYDIPYFIDK KREIIHNQIV ILIISAVEIL AKNWSYESVF RYLKTGLLDL EFDDIDILEN
YVLANGIRGR QWLDTKPWSF KINYGNFKED DSEEEQEYLN KINCIRDKVR EPILKLSNDI
KGKKKGRHIC EELYNFLCEL KIPEKVEELI IEFRNTDRLD KANEYSQIWD IVVDVLDQIV
DVLGEQSFGM EIFNEALEIG FSQYEIGVIP PALDQVLVSN ITRIKSYNIS ALYIAGVNDG
IFPVTISPEG IFTDQDRDEL KQNGLEIAPN TRSRAFEEQF LIYATLTIVD KYLTLTYSMS
DEEGKAKRPS VVISMIKKIF PKLQEKSNLL DASGYEGGIE AVNSPKVTFN MLISNIRRNL
GHRENINSLW IDVYRWYREH EIWNKRLDTV LGAFYYNNEI KISDLVKIRR LYGKHLNMSV
SRLEKFAQCP FGYFVQYGLK VKDRKMYSLS APDLGSFMHG ILERFSIGLK QKSLTWENVD
ERCCEENIND LVDNVLYDNP NSILNSSKKY KHVTDKLKKT LTRSVWLIAQ HMKKGRFIPR
AYELVFGEIG DFPPISIELD SGEKVSLTGK VDRVDTAKED IITYIRIVDY KSGTREFKLS
DVYYGFQLQL LIYLDAILTE FDKMTKGKSI PAGLLYFKLE DPIVRTKENI PDHEIEDRIT
KSLKMNGLLL NDVNVIRQMD TSMEKSSDII SVSIKKDGNL SKSKSSLATR KQFEILRKYV
RSTIADLCKK ILTGNIEVTP YKNKTKNGCS YCEYSAICQF DTSIKGNKYR IIEDKSDEEI
WKHIENKVQE
