DNAK_AGRFC
ID DNAK_AGRFC Reviewed; 633 AA.
AC P50019;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 27-MAR-2002, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Chaperone protein DnaK;
DE AltName: Full=HSP70;
DE AltName: Full=Heat shock 70 kDa protein;
DE AltName: Full=Heat shock protein 70;
GN Name=dnaK; OrderedLocusNames=Atu0122; ORFNames=AGR_C_195;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7592349; DOI=10.1128/jb.177.20.5952-5958.1995;
RA Segal G., Ron E.Z.;
RT "The dnaKJ operon of Agrobacterium tumefaciens: transcriptional analysis
RT and evidence for a new heat shock promoter.";
RL J. Bacteriol. 177:5952-5958(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
CC -!- FUNCTION: Acts as a chaperone. {ECO:0000250}.
CC -!- INDUCTION: By heat shock.
CC -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR EMBL; X87113; CAA60592.1; -; Genomic_DNA.
DR EMBL; AE007869; AAK85942.1; -; Genomic_DNA.
DR PIR; AE2591; AE2591.
DR PIR; E97373; E97373.
DR PIR; I39585; I39585.
DR RefSeq; NP_353157.1; NC_003062.2.
DR RefSeq; WP_006309946.1; NC_003062.2.
DR AlphaFoldDB; P50019; -.
DR SMR; P50019; -.
DR STRING; 176299.Atu0122; -.
DR PRIDE; P50019; -.
DR EnsemblBacteria; AAK85942; AAK85942; Atu0122.
DR GeneID; 66223133; -.
DR KEGG; atu:Atu0122; -.
DR PATRIC; fig|176299.10.peg.114; -.
DR eggNOG; COG0443; Bacteria.
DR HOGENOM; CLU_005965_2_1_5; -.
DR OMA; ISIKRHM; -.
DR PhylomeDB; P50019; -.
DR BioCyc; AGRO:ATU0122-MON; -.
DR Proteomes; UP000000813; Chromosome circular.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR Gene3D; 1.20.1270.10; -; 1.
DR Gene3D; 2.60.34.10; -; 1.
DR HAMAP; MF_00332; DnaK; 1.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR012725; Chaperone_DnaK.
DR InterPro; IPR018181; Heat_shock_70_CS.
DR InterPro; IPR029048; HSP70_C_sf.
DR InterPro; IPR029047; HSP70_peptide-bd_sf.
DR InterPro; IPR013126; Hsp_70_fam.
DR PANTHER; PTHR19375; PTHR19375; 1.
DR Pfam; PF00012; HSP70; 1.
DR SUPFAM; SSF100920; SSF100920; 1.
DR SUPFAM; SSF100934; SSF100934; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR PROSITE; PS00297; HSP70_1; 1.
DR PROSITE; PS00329; HSP70_2; 1.
DR PROSITE; PS01036; HSP70_3; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW Reference proteome; Stress response.
FT CHAIN 1..633
FT /note="Chaperone protein DnaK"
FT /id="PRO_0000078405"
FT REGION 605..633
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 610..633
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 198
FT /note="Phosphothreonine; by autocatalysis"
FT /evidence="ECO:0000250"
FT CONFLICT 293
FT /note="L -> P (in Ref. 1; CAA60592)"
FT /evidence="ECO:0000305"
FT CONFLICT 345
FT /note="Q -> R (in Ref. 1; CAA60592)"
FT /evidence="ECO:0000305"
FT CONFLICT 463
FT /note="G -> A (in Ref. 1; CAA60592)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 633 AA; 68080 MW; D53CC7E6EB3B50E3 CRC64;
MAKVIGIDLG TTNSCVAVMD GKDTKVIENA EGARTTPSMV AFSDDGERLV GQPAKRQAVT
NPTNTLFAVK RLIGRRYEDP TVEKDKALVP FEIVKGDNGD AWVKAQDKNY SPSQISAMIL
QKMKETAESY LGEKVEKAVI TVPAYFNDAQ RQATKDAGRI AGLDVLRIIN EPTAAALAYG
LDKKEGKTIA VYDLGGGTFD ISVLEIGDGV FEVKSTNGDT FLGGEDFDMR LVEYLAGEFK
KDQGIDLKND KLALQRLKEA AEKAKIELSS SQQTEINLPF ITADASGPKH LTLKLTRAKF
ESLVDDLVQR TVAPCKAALK DAGVTAAEID EVVLVGGMSR MPKVQEVVKQ LFGKEPHKGV
NPDEVVAMGA AIQAGVLQGD VKDVLLLDVT PLSLGIETLG GVFTRLIDRN TTIPTKKSQT
FSTAEDNQSA VTIRVSQGER EMAQDNKLLG QFDLVGLPPS PRGVPQIEVT FDIDANGIVQ
VSAKDKGTGK EQQIRIQASG GLSDADIEKM VKDAEANAEA DKKRRAGVEA KNQAESLIHS
TEKSVKEYGD KVSETDRKAI EDAIASLKTA VEAAEPDADD IQAKTQTLME VSMKLGQAIY
EAQQAEAGDA SAEGKDDVVD ADYEEIKDDK KSA
