DNAK_BACSU
ID DNAK_BACSU Reviewed; 611 AA.
AC P17820; P13343;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Chaperone protein DnaK;
DE AltName: Full=HSP70;
DE AltName: Full=Heat shock 70 kDa protein;
DE AltName: Full=Heat shock protein 70;
GN Name=dnaK; OrderedLocusNames=BSU25470;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / MB11;
RX PubMed=2110662; DOI=10.1093/nar/18.8.2172;
RA Wetzstein M., Schumann W.;
RT "Complete nucleotide sequence of the Bacillus subtilis dnaK gene.";
RL Nucleic Acids Res. 18:2172-2172(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / MB11;
RX PubMed=1339421; DOI=10.1128/jb.174.10.3300-3310.1992;
RA Wetzstein M., Voelker U., Dedio J., Loebau S., Zuber U., Schiesswohl M.,
RA Herget C., Hecker M., Schumann W.;
RT "Cloning, sequencing, and molecular analysis of the dnaK locus from
RT Bacillus subtilis.";
RL J. Bacteriol. 174:3300-3310(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA Kobayashi Y.;
RT "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT Bacillus subtilis genome containing the skin element and many sporulation
RT genes.";
RL Microbiology 142:3103-3111(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-572.
RC STRAIN=168 / MB11;
RX PubMed=2108432; DOI=10.1093/nar/18.5.1289;
RA Wetzstein M., Schumann W.;
RT "Nucleotide sequence of a Bacillus subtilis gene homologous to the grpE
RT gene of E. coli located immediately upstream of the dnaK gene.";
RL Nucleic Acids Res. 18:1289-1289(1990).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-214.
RC STRAIN=168 / MB24;
RX PubMed=2510131; DOI=10.1093/nar/17.20.8373;
RA Hearne C.M., Ellar D.J.;
RT "Nucleotide sequence of a Bacillus subtilis gene homologous to the dnaK
RT gene of Escherichia coli.";
RL Nucleic Acids Res. 17:8373-8373(1989).
RN [7]
RP PROTEIN SEQUENCE OF 2-8.
RC STRAIN=168 / IS58;
RX PubMed=8012595; DOI=10.1099/00221287-140-4-741;
RA Voelker U., Engelmann S., Maul B., Riethdorf S., Voelker A., Schmid R.,
RA Mach H., Hecker M.;
RT "Analysis of the induction of general stress proteins of Bacillus
RT subtilis.";
RL Microbiology 140:741-752(1994).
CC -!- FUNCTION: Acts as a chaperone. {ECO:0000250}.
CC -!- INDUCTION: By heat shock.
CC -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR EMBL; X52064; CAA36286.1; -; Genomic_DNA.
DR EMBL; M84964; AAA22528.1; -; Genomic_DNA.
DR EMBL; D84432; BAA12464.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14489.1; -; Genomic_DNA.
DR EMBL; X51477; CAA35842.1; -; Genomic_DNA.
DR EMBL; X16393; CAA34429.1; -; Genomic_DNA.
DR PIR; S09500; S09500.
DR RefSeq; NP_390425.1; NC_000964.3.
DR RefSeq; WP_004398786.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P17820; -.
DR SMR; P17820; -.
DR IntAct; P17820; 3.
DR MINT; P17820; -.
DR STRING; 224308.BSU25470; -.
DR jPOST; P17820; -.
DR PaxDb; P17820; -.
DR PRIDE; P17820; -.
DR EnsemblBacteria; CAB14489; CAB14489; BSU_25470.
DR GeneID; 937849; -.
DR KEGG; bsu:BSU25470; -.
DR PATRIC; fig|224308.179.peg.2768; -.
DR eggNOG; COG0443; Bacteria.
DR InParanoid; P17820; -.
DR OMA; ISIKRHM; -.
DR PhylomeDB; P17820; -.
DR BioCyc; BSUB:BSU25470-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR Gene3D; 1.20.1270.10; -; 1.
DR Gene3D; 2.60.34.10; -; 1.
DR HAMAP; MF_00332; DnaK; 1.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR012725; Chaperone_DnaK.
DR InterPro; IPR018181; Heat_shock_70_CS.
DR InterPro; IPR029048; HSP70_C_sf.
DR InterPro; IPR029047; HSP70_peptide-bd_sf.
DR InterPro; IPR013126; Hsp_70_fam.
DR PANTHER; PTHR19375; PTHR19375; 2.
DR Pfam; PF00012; HSP70; 1.
DR SUPFAM; SSF100920; SSF100920; 1.
DR SUPFAM; SSF100934; SSF100934; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR PROSITE; PS00297; HSP70_1; 1.
DR PROSITE; PS00329; HSP70_2; 1.
DR PROSITE; PS01036; HSP70_3; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Chaperone; Direct protein sequencing; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Stress response.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:8012595"
FT CHAIN 2..611
FT /note="Chaperone protein DnaK"
FT /id="PRO_0000078420"
FT REGION 579..611
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 593..611
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 173
FT /note="Phosphothreonine; by autocatalysis"
FT /evidence="ECO:0000250"
FT CONFLICT 99
FT /note="S -> R (in Ref. 6; CAA34429)"
FT /evidence="ECO:0000305"
FT CONFLICT 150
FT /note="A -> P (in Ref. 6; CAA34429)"
FT /evidence="ECO:0000305"
FT CONFLICT 166
FT /note="V -> L (in Ref. 6; CAA34429)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 611 AA; 66002 MW; E83CEA6E16A31593 CRC64;
MSKVIGIDLG TTNSCVAVLE GGEPKVIANA EGNRTTPSVV AFKNGERQVG EVAKRQSITN
PNTIMSIKRH MGTDYKVEIE GKDYTPQEVS AIILQHLKSY AESYLGETVS KAVITVPAYF
NDAERQATKD AGKIAGLEVE RIINEPTAAA LAYGLDKTDE DQTILVYDLG GGTFDVSILE
LGDGVFEVRS TAGDNRLGGD DFDQVIIDHL VSEFKKENGI DLSKDKMALQ RLKDAAEKAK
KDLSGVSSTQ ISLPFITAGE AGPLHLELTL TRAKFEELSS HLVERTMGPV RQALQDAGLS
ASEIDKVILV GGSTRIPAVQ EAIKKETGKE AHKGVNPDEV VALGAAIQGG VITGDVKDVV
LLDVTPLSLG IETMGGVFTK LIDRNTTIPT SKSQVFSTAA DNQTAVDIHV LQGERPMSAD
NKTLGRFQLT DIPPAPRGVP QIEVSFDIDK NGIVNVRAKD LGTGKEQNIT IKSSSGLSDE
EIERMVKEAE ENADADAKKK EEIEVRNEAD QLVFQTEKTL KDLEGKVDEE QVKKANDAKD
ALKAAIEKNE FEEIKAKKDE LQTIVQELSM KLYEEAAKAQ QAQGGANAEG KADDNVVDAE
YEEVNDDQNK K
