ADDB_DESAP
ID ADDB_DESAP Reviewed; 1165 AA.
AC B1I494;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B {ECO:0000255|HAMAP-Rule:MF_01452};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01452};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01452};
DE AltName: Full=ATP-dependent helicase/nuclease AddB {ECO:0000255|HAMAP-Rule:MF_01452};
GN Name=addB {ECO:0000255|HAMAP-Rule:MF_01452}; OrderedLocusNames=Daud_1277;
OS Desulforudis audaxviator (strain MP104C).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae;
OC Candidatus Desulforudis.
OX NCBI_TaxID=477974;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MP104C;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Bruce D., Pitluck S., Lowry S.R., Larimer F., Land M.L.,
RA Hauser L., Kyrpides N., Ivanova N.N., Richardson P.;
RT "Complete sequence of chromosome of Desulforudis audaxviator MP104C.";
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddB nuclease domain is not
CC required for chi fragment generation; this subunit has 5' -> 3'
CC nuclease activity. {ECO:0000255|HAMAP-Rule:MF_01452}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- SUBUNIT: Heterodimer of AddA and AddB. {ECO:0000255|HAMAP-
CC Rule:MF_01452}.
CC -!- SIMILARITY: Belongs to the helicase family. AddB/RexB type 1 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01452}.
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DR EMBL; CP000860; ACA59788.1; -; Genomic_DNA.
DR RefSeq; WP_012302373.1; NC_010424.1.
DR AlphaFoldDB; B1I494; -.
DR SMR; B1I494; -.
DR STRING; 477974.Daud_1277; -.
DR EnsemblBacteria; ACA59788; ACA59788; Daud_1277.
DR KEGG; dau:Daud_1277; -.
DR eggNOG; COG3857; Bacteria.
DR HOGENOM; CLU_007838_0_0_9; -.
DR OMA; DRLENYV; -.
DR OrthoDB; 1283891at2; -.
DR Proteomes; UP000008544; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0008409; F:5'-3' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 3.
DR HAMAP; MF_01452; AddB_type1; 1.
DR InterPro; IPR014140; DNA_helicase_suAddB.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02773; addB_Gpos; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; ATP-binding; DNA damage; DNA repair; Exonuclease; Hydrolase; Iron;
KW Iron-sulfur; Metal-binding; Nuclease; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..1165
FT /note="ATP-dependent helicase/deoxyribonuclease subunit B"
FT /id="PRO_0000379188"
FT DOMAIN 1..298
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT DOMAIN 279..584
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 8..15
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 800
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1119
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1122
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1128
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
SQ SEQUENCE 1165 AA; 129720 MW; 36FACEDE3303822E CRC64;
MALRFILGRA GTGKTRLCLE EIRKELRQAP DGPPLVFLVP EQATFQTEYA LALTPGLSGM
FRAQVLSFRR LAWRVFSEVG GAARPHVGDV GKRMMLARIL ARRRNELRLF GRAAGQYGFS
GTLAGVLSEL KTYLVTPEAL EQAVRMLPGT AETDSLQAKL EDLRLLYTDF ERELAGKYID
PDDYLTLLAA RLGESPTLRA AEVWVDGFAG FTPQEYAVLE ALLKAAARVN VALCLDPRSG
RREDLELFQV TRDTRARLAE LARKNGVELE RPVELAGPPA RFRANPALAH LEREFFRRPT
KKFAGPPENL RLVAAASRRA EVEGAAREIL RLSRERGWRW RDVSLVVRNL ADYHELVATV
FADYGIPCFI DRKRPVRHHP LVELIRSALE TVTENWTYDP VFRYLKTDLV PVSRGDVDLL
ENYVLAHGIK GAKWADPEDW HYRRSDALNR PVPNGTGAPA PSDRDRYLRE KVNRIRRRAA
RHLLDFQRRV QAAGTVRELT AALYDLLSDL DVPGRIEEWS REAETEGRLE AAREHRQLWD
GVVELLDQVV ETLGDEALTP EEYGRILDSG MDGLQLALIP PALDQVLVGS LERSRNPDIR
AAFVLGVSEG VLPGRHGGTG LFTDREREVL LAAGLEVAPD TRRKVYEEQY LVYIALTRAG
HYLWVSYPLA DEEGGALAPS SVIPRLRELF PGLREETLAL EPEGTAPGAD LPYVTAPGRT
LGFLVTRLRD WKSGVTVDPV WWSVFNWFAA HAGWRERCAP VLAGLFYENR EPRLDPELTP
ELYGSRLVVS VSRLEEFRGC PFAHFARYAL RLKERDVCRL AAPDIGLFFH AALKTFEDRL
REAGMEWDAL ESADCTRLAS AVVEELAPQL QSEVLLSSPR LRFLTGKLER VVERTAGVMA
AHARHSRFQP VAVEVAFGPG RDVPGPAYPL PGGGSVELAG RIDRVDVAHT DNAAYLRVID
YKAGPRSLAL DDVYYGLNLQ LLVYLEAGLR EAAALAGREC RPAGAFYFRV QNPLLKGGTP
VPPAEVAPRL LKAFRLQGLV LDDPALLRLM DDAIGSESAI VPAGLKQDGT VKKKPGVVSA
AQLERLQEHV RRVVAETAAE IQAGVVAIAP YRKGQANACR YCAFKPVCAF DPLLESNRYR
QLRALTAGEL WRLLGLPEED EPGDE
