ADDB_EXIS2
ID ADDB_EXIS2 Reviewed; 1143 AA.
AC B1YKM7;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B {ECO:0000255|HAMAP-Rule:MF_01452};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01452};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01452};
DE AltName: Full=ATP-dependent helicase/nuclease AddB {ECO:0000255|HAMAP-Rule:MF_01452};
GN Name=addB {ECO:0000255|HAMAP-Rule:MF_01452}; OrderedLocusNames=Exig_0729;
OS Exiguobacterium sibiricum (strain DSM 17290 / CIP 109462 / JCM 13490 /
OS 255-15).
OC Bacteria; Firmicutes; Bacilli; Bacillales;
OC Bacillales Family XII. Incertae Sedis; Exiguobacterium.
OX NCBI_TaxID=262543;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17290 / CIP 109462 / JCM 13490 / 255-15;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Kiss H., Chertkov O., Monk C.,
RA Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F.,
RA Land M., Hauser L., Kyrpides N., Mikhailova N., Vishnivetskaya T.,
RA Rodrigues D.F., Gilichinsky D., Tiedje J., Richardson P.;
RT "Complete sequence of chromosome of Exiguobacterium sibiricum 255-15.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddB nuclease domain is not
CC required for chi fragment generation; this subunit has 5' -> 3'
CC nuclease activity. {ECO:0000255|HAMAP-Rule:MF_01452}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- SUBUNIT: Heterodimer of AddA and AddB. {ECO:0000255|HAMAP-
CC Rule:MF_01452}.
CC -!- SIMILARITY: Belongs to the helicase family. AddB/RexB type 1 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01452}.
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DR EMBL; CP001022; ACB60210.1; -; Genomic_DNA.
DR RefSeq; WP_012369634.1; NC_010556.1.
DR AlphaFoldDB; B1YKM7; -.
DR SMR; B1YKM7; -.
DR STRING; 262543.Exig_0729; -.
DR PRIDE; B1YKM7; -.
DR EnsemblBacteria; ACB60210; ACB60210; Exig_0729.
DR KEGG; esi:Exig_0729; -.
DR eggNOG; COG3857; Bacteria.
DR HOGENOM; CLU_007838_0_0_9; -.
DR OMA; DRLENYV; -.
DR OrthoDB; 1283891at2; -.
DR Proteomes; UP000001681; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0008409; F:5'-3' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 3.
DR Gene3D; 3.90.320.10; -; 1.
DR HAMAP; MF_01452; AddB_type1; 1.
DR InterPro; IPR014140; DNA_helicase_suAddB.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02773; addB_Gpos; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; ATP-binding; DNA damage; DNA repair; Exonuclease; Hydrolase; Iron;
KW Iron-sulfur; Metal-binding; Nuclease; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..1143
FT /note="ATP-dependent helicase/deoxyribonuclease subunit B"
FT /id="PRO_0000379190"
FT DOMAIN 1..274
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT DOMAIN 267..565
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 7..14
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 782
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1104
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1107
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1113
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
SQ SEQUENCE 1143 AA; 131859 MW; C5B6CB6325126CFB CRC64;
MNYMHLGRAG TGKTTQLIER VIDQLTEQPL GPSIYFIVPD QMSFEMERRI ATDPRLSGLV
RMEVMSLRRF AFHMIRDYGN QVIPFLDETG TQLLLRQVVE EAEEELKIFK RTKNMPGFYK
GLDELIASFK RSLVNPDMLR QISGASPERS PKLNDLALIY ERFTERISDK ALHADDYFTT
LIELLPKADL SDVSVFVDGF YEFSLQEQQV LLRLMELTKD MHLSFTLDVE DVYTKQSEFG
VSQRCYMQLI EQMKERQIAH EDIYYGQTVK FQSTGLRMLE QALLSPGYPP TDEEVTGVTV
SAAVNRQVET EAAVRKAIQL VRHDGYRFKD IAFLVRHLEP YADHLERAFQ LYDIPYFLDQ
RESMVHHPIV ELVHAVLEIV MTGYREESVF RLLKTELIPL PAEDPRLALD RLETFVLERG
IKGSMWKKPW QLKRRLADEI SLTDAELQEE LELNEWRQFL VEAIEPLEKR FKASKTMSEY
TRALYRFLEE HHMTDRLTVW KQQALEVNEL SQAREHDQVY EAVLLLFEQL EAAAPEATLT
TELFVQMVET GLESLRFSLV PPSLDQVIAT DYVRGRLQQV KVVFLLGAND GLIPLVEDQS
KLLSEGDHDF LHDQGIPVGK ASLDVFDDEL YYLYQGVTAP SESLHISYAL VDEDGKALQP
ASIVKQIKHQ LLQDRPVKTY FAEAGDHAPD DQLQFVTSPE RAAAAAAIEL RRLQRRYPIQ
PSWFDVYNRL LENGRGRERM ALFSNALFYQ NQAEPLPEDL ARNLYGDTIK ASVSRFETYN
ACSYKHFARY GLRLKERKLY KFEAPDIGNL FHGALNDLSM NIKASGKRWR ELDDQSCSNL
AKEAVEKVTP EIQNAILMSS NRFGYIKKKL TDVVEQTAKM LVKQAERSEF EPDLFEISFG
NAMFPPLRFT LPDGTEIEFT GRIDRVDQAT IGDQLYVRIV DYKSSAKELD FAEIYYGLAI
QMLLYLKTVV EQSEVLFNQQ AKPAGALYFH VKNPMMRGDL SADEIERNQL LLESYQMQGV
IVENDEVLRA MDQIAYDERK KSPLVKVTFT KTGLHKSHTK GVVKEEELTA LMDHAWDELK
TSSQSMYAGD IAIDPFDYQE RTPCTFCEYR SVCQFDQSLG NDYRQLNPLS EKEVLERLKE
ETE
