ADDB_GEOTN
ID ADDB_GEOTN Reviewed; 1167 AA.
AC A4IKW6;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B {ECO:0000255|HAMAP-Rule:MF_01452};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01452};
DE EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01452};
DE AltName: Full=ATP-dependent helicase/nuclease AddB {ECO:0000255|HAMAP-Rule:MF_01452};
GN Name=addB {ECO:0000255|HAMAP-Rule:MF_01452}; OrderedLocusNames=GTNG_0588;
OS Geobacillus thermodenitrificans (strain NG80-2).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX NCBI_TaxID=420246;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NG80-2;
RX PubMed=17372208; DOI=10.1073/pnas.0609650104;
RA Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X.,
RA Han W., Peng X., Liu R., Wang L.;
RT "Genome and proteome of long-chain alkane degrading Geobacillus
RT thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007).
CC -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA helicase
CC and an ATP-dependent, dual-direction single-stranded exonuclease.
CC Recognizes the chi site generating a DNA molecule suitable for the
CC initiation of homologous recombination. The AddB nuclease domain is not
CC required for chi fragment generation; this subunit has 5' -> 3'
CC nuclease activity. {ECO:0000255|HAMAP-Rule:MF_01452}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01452};
CC -!- SUBUNIT: Heterodimer of AddA and AddB. {ECO:0000255|HAMAP-
CC Rule:MF_01452}.
CC -!- SIMILARITY: Belongs to the helicase family. AddB/RexB type 1 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01452}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABO65970.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000557; ABO65970.1; ALT_INIT; Genomic_DNA.
DR AlphaFoldDB; A4IKW6; -.
DR SMR; A4IKW6; -.
DR STRING; 420246.GTNG_0588; -.
DR EnsemblBacteria; ABO65970; ABO65970; GTNG_0588.
DR KEGG; gtn:GTNG_0588; -.
DR eggNOG; COG3857; Bacteria.
DR HOGENOM; CLU_007838_0_0_9; -.
DR Proteomes; UP000001578; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0008409; F:5'-3' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 3.
DR HAMAP; MF_01452; AddB_type1; 1.
DR InterPro; IPR014140; DNA_helicase_suAddB.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR02773; addB_Gpos; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; ATP-binding; DNA damage; DNA repair; Exonuclease; Hydrolase; Iron;
KW Iron-sulfur; Metal-binding; Nuclease; Nucleotide-binding.
FT CHAIN 1..1167
FT /note="ATP-dependent helicase/deoxyribonuclease subunit B"
FT /id="PRO_0000379192"
FT DOMAIN 1..359
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT DOMAIN 282..588
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 8..15
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 803
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1125
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1128
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
FT BINDING 1134
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01452"
SQ SEQUENCE 1167 AA; 133929 MW; 23C00BCE7F5445C0 CRC64;
MSLRFLLGRS GSGKTTACLE EIRRQLQEDP KGRTIVYLVP EQMTFQCEYA LIHTEGTEGM
IRAQVFSFPR LAWRVLQETG GMNRYHVHDV GVQMMIRKII EQRKQELKLF GRAADKSGFV
EQLHEMIAEC KRYCLTPDEL RRHAGVLESG SGQPGRRLLA DKLSDVALVY EELERSLLGH
YLDSEDYLRL LVEHIPRSSY LHGADIYIDG FHHFSPQEYM VIEQLLHRCR RVTVCLTADR
PYDVGMPDEL DLFYLPAQTY RQLRELALAN DIMIESPVVL SVNRRHQDKA LAHLEEQFHR
RPLLPYEAET DAICLYEAAN RRAEIEAVAR EIIRLVRDEG ARYRDIALII RQTEAYRDLV
KTVFFDFDIP YFMDEKEPMH YHPLIELVRA ALETVVTRWR YEAVFRAVKT DLLFPVDGDL
AMWREAADKL ENYVLAHGVK GEKWTSDERW TYRRYQALDG LNVPQTDEER QFEDKLNEWR
EALAAPLRRL ERRLRRAADG RGLCTALYLF LEELQIPKKL EQMSAQAEAD GRLVEARQHE
QAWNAVVDLL DQYVEMLGAE PLPLAEFAKI IEAGLDRLEF ALVPPAIDQV IVAQLDRSRL
IDVKYAFIIG VNDGVIPAKV KDEGLVAEVE REQLRELGVA LAPGGREQLF YDPFFVYLAL
ACPSRRLYVT YPLADGEGKA LMPSPLIKQL VQLFPRVSVH LCGNDPFDAP EEKPETFVTV
PRATQTYLIS QLRAWKRNYG IDPLWWDVYN VLISHPEWRA RVEQAVSGLF YTNQAVLKRE
WSRRLYGKKI QASVSRMEQF QKCPYAHFAS HGLRLKERNV FRLEAPDVGQ LFHAAIKQIA
DRVREQHLDW KQLSRSECER LSAEAVERIA PLIQQQVLSS SNRYEYMKRK LKNVVARTTH
VLSEHARASG FAPVGFELSF GPGGDLPPLR FQLRDGTVME LVGRIDRVDK AESSQGVLLR
IIDYKSSAKT LDLTEVYYGL ALQMFTYLDI VLTYAEQLVG EPALPAGVLY FHIHNPIIQA
KQWLDNEMEV ARKLLEPFRM RGLLLADAET IRLMDSQTEN GQWSLIVPAQ LTRTGAIHSR
SSVASASDFA ALRQHVRRLF VDIGGQIADG VVSIAPYKLK NKTACEFCAF KPVCQFDEAL
SGNGYRKLTP QTKDAVIETL GKREEES
