ID   DNAK_CLOD6              Reviewed;         615 AA.
AC   Q182E8;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Chaperone protein DnaK {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=HSP70 {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock 70 kDa protein {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock protein 70 {ECO:0000255|HAMAP-Rule:MF_00332};
GN   Name=dnaK {ECO:0000255|HAMAP-Rule:MF_00332}; OrderedLocusNames=CD630_24610;
OS   Clostridioides difficile (strain 630) (Peptoclostridium difficile).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae;
OC   Clostridioides.
OX   NCBI_TaxID=272563;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=630;
RX   PubMed=16804543; DOI=10.1038/ng1830;
RA   Sebaihia M., Wren B.W., Mullany P., Fairweather N.F., Minton N.,
RA   Stabler R., Thomson N.R., Roberts A.P., Cerdeno-Tarraga A.M., Wang H.,
RA   Holden M.T.G., Wright A., Churcher C., Quail M.A., Baker S., Bason N.,
RA   Brooks K., Chillingworth T., Cronin A., Davis P., Dowd L., Fraser A.,
RA   Feltwell T., Hance Z., Holroyd S., Jagels K., Moule S., Mungall K.,
RA   Price C., Rabbinowitsch E., Sharp S., Simmonds M., Stevens K., Unwin L.,
RA   Whithead S., Dupuy B., Dougan G., Barrell B., Parkhill J.;
RT   "The multidrug-resistant human pathogen Clostridium difficile has a highly
RT   mobile, mosaic genome.";
RL   Nat. Genet. 38:779-786(2006).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000255|HAMAP-Rule:MF_00332}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock. {ECO:0000255|HAMAP-
CC       Rule:MF_00332}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00332}.
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DR   EMBL; AM180355; CAJ69348.1; -; Genomic_DNA.
DR   RefSeq; WP_003430934.1; NZ_CP010905.2.
DR   RefSeq; YP_001088975.1; NC_009089.1.
DR   AlphaFoldDB; Q182E8; -.
DR   SMR; Q182E8; -.
DR   STRING; 272563.CD630_24610; -.
DR   EnsemblBacteria; CAJ69348; CAJ69348; CD630_24610.
DR   GeneID; 66354859; -.
DR   KEGG; cdf:CD630_24610; -.
DR   KEGG; pdc:CDIF630_02707; -.
DR   PATRIC; fig|272563.120.peg.2600; -.
DR   eggNOG; COG0443; Bacteria.
DR   OMA; ISIKRHM; -.
DR   PhylomeDB; Q182E8; -.
DR   BioCyc; PDIF272563:G12WB-2616-MON; -.
DR   Proteomes; UP000001978; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 2.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Stress response.
FT   CHAIN           1..615
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_1000059542"
FT   REGION          573..615
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        573..592
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        598..615
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         175
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00332"
SQ   SEQUENCE   615 AA;  66468 MW;  B322FD9AB0BF986E CRC64;
     MGKIIGIDLG TTNSCVAVLE GGEAQIIANS EGMRTTPSVV AFTKDGERIV GEPAKRQAVT
     NADKTITSIK THMGTDYKVN IDGKSYTPQE ISAIILQKLK SDAESYLGQT VTEAVITVPA
     YFTDAQRQAT KDAGRIAGLD VKRIINEPTA AALAYGMDKL DQEKKILVFD LGGGTFDVSI
     LEIGDGTFEV LATAGNNRLG GDDFDQIVID YLAEEFKKAE GVDLRNDKMA LQRLKEAAEK
     AKKELSSTMS SNINLPFITA TAEGPKHLNI DLSRAKFEEL TRGLVEKTME PTKTALQDAG
     LSTGDIDDVL LVGGSTRIPA VQEAVKKFIG KEPHKGINPD ECVAAGASIQ AGVLAGDVKD
     LLLLDVTPLS LGIETMGNVM TKIIERNTTI PTKKSQIFST AADNQTAVDI HVLQGERSMA
     YDNTTLGRFQ LTDIPPAQRG IPQIEVTFDI DANGIVNVSA KDLGTGKEQK ITITSNTNLS
     EAEIEQKIKE AEMNAEADKQ KKEKIEAFNQ AESTIYQTEK TLNELGDKIS SGEKEDIEKA
     IADLKAVKDN QDATAEELKK ATDEVMTKFQ KVSQEMYQKA AQEQQAAQGA EQAQDNGPKD
     DNVVDADFKE VDEDK