ID   DNAK_ENTFA              Reviewed;         609 AA.
AC   Q835R7;
DT   19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Chaperone protein DnaK {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=HSP70 {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock 70 kDa protein {ECO:0000255|HAMAP-Rule:MF_00332};
DE   AltName: Full=Heat shock protein 70 {ECO:0000255|HAMAP-Rule:MF_00332};
GN   Name=dnaK {ECO:0000255|HAMAP-Rule:MF_00332}; OrderedLocusNames=EF_1308;
OS   Enterococcus faecalis (strain ATCC 700802 / V583).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=226185;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700802 / V583;
RX   PubMed=12663927; DOI=10.1126/science.1080613;
RA   Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA   Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA   Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C.,
RA   DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C.,
RA   Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M.,
RA   Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT   "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT   faecalis.";
RL   Science 299:2071-2074(2003).
CC   -!- FUNCTION: Acts as a chaperone. {ECO:0000255|HAMAP-Rule:MF_00332}.
CC   -!- INDUCTION: By stress conditions e.g. heat shock. {ECO:0000255|HAMAP-
CC       Rule:MF_00332}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC       {ECO:0000255|HAMAP-Rule:MF_00332}.
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DR   EMBL; AE016830; AAO81100.1; -; Genomic_DNA.
DR   RefSeq; NP_815030.1; NC_004668.1.
DR   RefSeq; WP_002357825.1; NZ_KE136528.1.
DR   AlphaFoldDB; Q835R7; -.
DR   SMR; Q835R7; -.
DR   STRING; 226185.EF_1308; -.
DR   PRIDE; Q835R7; -.
DR   EnsemblBacteria; AAO81100; AAO81100; EF_1308.
DR   GeneID; 60893691; -.
DR   KEGG; efa:EF1308; -.
DR   PATRIC; fig|226185.45.peg.2193; -.
DR   eggNOG; COG0443; Bacteria.
DR   HOGENOM; CLU_005965_2_4_9; -.
DR   OMA; ISIKRHM; -.
DR   Proteomes; UP000001415; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   HAMAP; MF_00332; DnaK; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR012725; Chaperone_DnaK.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 2.
DR   Pfam; PF00012; HSP70; 2.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   TIGRFAMs; TIGR02350; prok_dnaK; 1.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chaperone; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Stress response.
FT   CHAIN           1..609
FT                   /note="Chaperone protein DnaK"
FT                   /id="PRO_0000078462"
FT   REGION          577..609
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         174
FT                   /note="Phosphothreonine; by autocatalysis"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00332"
SQ   SEQUENCE   609 AA;  65584 MW;  0A28957EA671D4E3 CRC64;
     MSKIIGIDLG TTNSAVAVLE GGEAKIIANP EGNRTTPSVV SFKNGEIQVG EVAKRQAVTN
     PNTISSIKRH MGEAGYKVDV EGKSYTPQEV SAMILQYLKG FAEDYLGEKV EKAVITVPAY
     FNDAQRQATK DAGKIAGLEV ERIVNEPTAA ALAYGLDKTD KDEKILVFDL GGGTFDVSIL
     ELGDGVFDVL STAGDNNLGG DDFDNKIIDY MVAEFKKENG IDLANDKMAL QRLKDAAEKA
     KKDLSGVTST QISLPFITAG EAGPLHLEMN LTRAKFDELT SDLVERTKVP VRQALKDAGL
     NPSEIDEVIL VGGSTRIPAV VEAVRKETNK EPNKSVNPDE VVAMGAAIQG GVITGDVKDV
     VLLDVTPLSL GIETMGGVFT KLIDRNTTIP TSKSQVFSTA ADNQPAVDIH VLQGERPMAA
     DNKTLGRFQL TDIPAAPRGV PQIEVSFDID KNGIVNVRAK DLGTQKEQTI TIKSSSGLSD
     DEIERMVKDA EANAEADKQR KEEVDLRNDA DALLFTVDKT LKELEGKVDA EEVKKAEDAR
     DELKAAIEAN DIEQMKAKRD SLNEIVQNLT VKLYEQAAQQ QAQENPEAAQ GGADDVVDAD
     FEEVNGDDK